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Mitogen-activated protein kinase in human eggs

Published online by Cambridge University Press:  01 May 1999

Qing-Yuan Sun ,
Zeev Blumenfeld ,
Sara Rubinstein ,
Shlomit Goldman ,
Yael Gonen  and
Haim Breitbart
Qing-Yuan Sun
Affiliation:
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080 China.
Zeev Blumenfeld
Affiliation:
Department of Obstetrics and Gynecology, Rambam Medical Center, The B. Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel. IVF Unit HMC, Haifa 31096, Israel.
Sara Rubinstein
Affiliation:
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Shlomit Goldman
Affiliation:
Department of Obstetrics and Gynecology, Rambam Medical Center, The B. Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel. IVF Unit HMC, Haifa 31096, Israel.
Yael Gonen
Affiliation:
Department of Obstetrics and Gynecology, Rambam Medical Center, The B. Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel. IVF Unit HMC, Haifa 31096, Israel.
Haim Breitbart
Affiliation:
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
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Abstract

Mitogen-activated protein (MAP) kinase in human eggs has been investigated by using immunoblotting with both anti-Active MAPK and anti-ERK2 antibodies. The results showed that the main form of MAP kinase was p42ERK2. It was in a dephosphorylated form in oocytes at the germinal vesicle stage, but fully phosphorylated in unfertilised mature eggs. MAP kinase phosphorylation was significantly decreased when pronuclei were formed after intracytoplasmic sperm injection. Neither MAP kinase expression nor activity was detected in morphologically degenerated eggs. Although MAP kinase still existed in early embryos arrested at the 8-cell or morula stages, little, if any, activity could be detected. These data suggest that MAP kinase may play an important role in the cell cycle regulation of human eggs, as in other mammalian species.

Keywords

Cell cycleEggHumanMAP kinaseOocyte
Type
Research Article
Information
Zygote , Volume 7 , Issue 2 , May 1999 , pp. 181 - 185
Copyright
1999 Cambridge University Press

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