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Microfilament assembly and cortical granule distribution during maturation, parthenogenetic activation and fertilisation in the porcine oocyte

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Billy N. Day ,
Hoon Taek Lee  and
Kil-Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Billy N. Day
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Hoon Taek Lee
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Kil-Saeng Chung*
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
*
Dr Kil-Saeng Chung, Kon-Kuk University, Department of Animal Science, Kwangjin-gu, Mojin-dong, Seoul 143-701, South Korea. Telephone: 82-2-450-3672. Fax: 82-2-455-5305.
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Summary

In this study we imaged integral changes in microfilament assembly and cortical granule distribution, and examined effects of microfilament inhibitor on the cortical granule distribution during oocyte maturation, parthenogenetic activation and in vitro fertilisation in the pig. The microfilament assembly and cortical granule distribution were imaged with fluorescent-labelled lectin and rhodamine-labelled phalloidin under laser scanning confocal microscopy. At the germinal vesicle stage, cortical granule organelles were located around the cell cortex and were present as a relatively wide area on the oolemma. Microfilaments were also observed in a wide uniform area around the cell cortex. Following germinal vesicle breakdown, microfilaments concentrated in the condensed chromatin and cortical granules were observed in the cortex. Treatment with cytochalasin B inhibited microfilament polymerisation and prevented movement of cortical granules to the cortex. Cortical granule exudation following sperm penetration was evenly distributed in the entire perivitelline space. These results suggest that the microfilament assembly is involved in the distribution, movement and exocytosis of cortical granules during maturation and fertilisation.

Keywords

Cortical granuleFertilisationMaturationMicrofilamentsOocytePig
Type
Article
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 145 - 149
Copyright
Copyright © Cambridge University Press 1996

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References

Cran, D.G. & Cheng, W.T.K. (1985). Changes in cortical granules during porcine oocyte maturation. Gamete. Res. 11, 311–19.Google Scholar
Cran, D.G. & Cheng, W.T.K. (1986). The cortical reaction in pig oocytes during in vivo and in vitro fertilization. Gamete. Res. 13, 241–51.CrossRefGoogle Scholar
Cran, D.G., Moor, R.M. & Hay, M.F. (1980). Fine structure of the sheep oocyte during antral follicle development. J. Reprod. Fertil. 59, 125–31.CrossRefGoogle ScholarPubMed
Dandekar, P. & Talbot, P. (1992). Perivitelline space of mammalian oocytes: extracellular matrix of unfertilized oocytes and formation of a cortical granule envelope following fertilization. Mol. Reprod. Dev. 31, 135–43.Google Scholar
Ducibella, T., Kurasawa, S., Rangarajan, S., Korf, G.S. & Schultz, R.M. (1990). Precocious loss of cortical granules during mouse oocyte meiotic maturation and correlation with an egg-induced modification of the zona pellucida. Dev. Biol. 137, 46–55.Google Scholar
Funahashi, H., Stumpf, T.T., Terlouw, S.L. & Day, B.N. (1993). Effects of electrical stimulation before or after in vitro fertilization on sperm penetration and pronuclear formation of pig oocytes. Mol. Reprod. Dev. 36, 361–7.Google Scholar
Funahashi, H., Cantley, T.C., Stumpf, T.T., Terlouw, S.L. & Day, B.N. (1994). In vitro development of in vitro matured porcine oocytes following chemical activation or in vitro fertilization. Biol. Reprod. 50, 1072–7.CrossRefGoogle ScholarPubMed
Funahashi, H., Stumpf, T.T., Cantley, T.C., Kim, N.-H. & Day, B.N. (1995). Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilisation and/or electrical activation. Zygote 3, 273–81.CrossRefGoogle ScholarPubMed
Funahashi, H., Kim, N.-H, Stumpf, T.T., Terlouw, S.L. & Day, B.N. (1996). The presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol. Reprod. 54, 1412–19.CrossRefGoogle ScholarPubMed
Hyttel, P., Callesen, H. & Greve, T. (1989). A comparative ultrastructural study of in vivo versus in vitro fertilization of bovine oocytes. Anat. Embryol. 179, 435–42.Google Scholar
Kim, N.-H, Funahashi, H., Abeydeera, L.R., Moon, S.J., Prather, R.S., Schatten, G. & Day, B.N. (1996 a). Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during in vitro fertilization. J. Reprod. Fertil. 107, 79–86.Google Scholar
Kim, N.-H, Funahashi, H., Prather, R.S., Schatten, G. & Day, B.N. (1996 b). Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation. Mol. Reprod. Dev. 43, 248–55.Google Scholar
Kim, N.-H., Moon, S.J., Prather, R.S. & Day, B.N. (1996 c). Cytoskeletal alteration in aged porcine oocytes and parthenogenesis. Mol. Reprod. Dev. 43, 513–18.Google Scholar
Kim, N.-H., Simerly, C., Funahashi, H., Schatten, G. & Day, B.N. (1996 d). Microtubule organization in the porcine oocyte during fertilization and parthenogenesis. Biol. Reprod. 54, 1397–404.Google Scholar
Kim, N.-H., Chung, K.S. & Day, B.N. (1996 e). The distribution and requirement of microtubules and microfilaments during fertilization and parthenogenesis in porcine oocytes. J. Reprod. Fertil, in press.Google Scholar
Kruip, T.A., Cran, D.J., Van Beneden, T.H. &Dieleman, S.J. (1983). Structural changes in bovine oocytes during final maturation in vivo. Gamete Res. 8, 29–47.Google Scholar
Le Guen, P., Crozet, N., Huneau, D. & Gall, L. (1989). Distribution and role of microfilaments during early events of sheep fertilization. Gamete Res. 22, 411–25.CrossRefGoogle ScholarPubMed
Longo, F.J. (1987). Actin-plasma membrane association in mouse eggs and oocytes. J. Exp. Zool. 243, 299–309.Google Scholar
Maro, B., Johnson, M.H., Pickering, S.J. & Flach, G. (1984). Changes in the actin distribution during fertilization of the mouse egg. J. Embryol. Exp. Morphol. 81, 211–37.Google Scholar
Naito, K., Daen, F.P. & Toyoda, Y. (1992). Comparison of histone H1 kinase activity during meiotic maturation between two types of porcine oocytes matured in different media in vitro. Biol. Reprod. 47, 43–7.Google Scholar
Niwa, K. (1993). Effectiveness of in vitro fertilization techniques in pigs. J. Reprod. Fertil. (Suppl.) 48, 49–59.Google Scholar
Petters, R.M. & Wells, K.D. (1993). Culture of pig embryos. J. Reprod. Fertil. (Suppl.) 48, 61–73.Google ScholarPubMed
Simerly, C. & Schatten, G.(1993). Techniques for localization of specific molecules in oocytes and embryos. Methods Enzymol. 225, 516–52.CrossRefGoogle ScholarPubMed
Sirard, M.A., Dubuc, A., Bolamba, D., Zeng, Y., Coenen, K. (1993). Follicle-oocyte-sperm interactions in vivo and in vitro in pigs. J. Reprod. Fertil. (Suppl.) 48, 3–16.Google Scholar
Sun, F.Z., Hoyland, J., Huang, X., Mason, W. & Moor, R.M. (1992). A comparison of intracellular changes in porcine eggs after fertilization and electroactivation. Development 115, 947–56.CrossRefGoogle ScholarPubMed
Yoshida, M., Cran, D.G. & Pursel, V. (1993). Confocal and fluorescence microscopic study using lectins of the distribution of cortical granules during the maturation and fertilization of pig oocytes. Mol. Reprod. Dev. 36, 462–8.Google Scholar