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Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes

Published online by Cambridge University Press:  14 May 2013

S. Bilotto ,
R. Boni ,
G.L. Russo  and
M.B. Lioi
S. Bilotto
Affiliation:
Department of Sciences, University of Basilicata, 85100 Potenza, Italy. Institute of Food Sciences, National Research Council, 83100 Avellino, Italy.
R. Boni*
Affiliation:
University of Basilicata, Via dell'Ateneo Lucano, 10–85100–Potenza, Italy.
G.L. Russo
Affiliation:
Institute of Food Sciences, National Research Council, 83100 Avellino, Italy.
M.B. Lioi
Affiliation:
Department of Sciences, University of Basilicata, 85100 Potenza, Italy.
*
All correspondence to: Raffaele Boni. University of Basilicata, Via dell'Ateneo Lucano, 10–85100–Potenza, Italy. Tel: +39 0971 205017. Fax: +39 0971 205099. e-mail: [email protected]
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Summary

Several genetic and physiological factors increase the risk of DNA damage in mammalian oocytes. Two critical events are: (i) meiosis progression, from maturation to fertilization, due to extensive chromatin remodelling during genome decondensation; and (ii) aging, which is associated with a progressive oxidative stress. In this work, we studied the transcriptional patterns of three genes, RAD51, APEX-1 and MLH1, involved in DNA repair mechanisms. The analyses were performed by real-time quantitative PCR (RT-qPCR) in immature and in vitro matured oocytes collected from 17 ± 3-month-old heifers and 94 ± 20-month-old cows. Batches of 30–50 oocytes for each group (three replicates) were collected from ovarian follicles of slaughtered animals. The oocytes were freed from cumulus cells at the time of follicle removal, or after in vitro maturation (IVM) carried out in M199 supplemented with 10% fetal calf serum, 10 IU luteinising hormone (LH)/ml, 0.1 IU follicle-stimulating hormone (FSH)/ml and 1 μg 17β-oestradiol/ml. Total RNA was extracted by Trizol method. The expression of bovine GAPDH gene was used as the internal standard, while primers for bovine RAD51, APEX-1 and MLH1 genes were designed from DNA sequences retrieved from GenBank. Results obtained indicate a clear up-regulation of RAD51, APEX-1 and MLH1 genes after IVM, ranging between two- and four-fold compared with germinal vesicle (GV) oocytes. However, only RAD51 showed a significant transcript increase between the immature oocytes collected from young or old individuals. This finding highlights RAD51 as a candidate gene marker for discriminating bovine immature oocytes in relation to the donor age.

Keywords

APEX-1BovineDNA repair genesIVMMLH1OocyteRAD5
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 11 - 18
Copyright
Copyright © Cambridge University Press 2013 

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