Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-18T17:55:37.412Z Has data issue: false hasContentIssue false
  • English
  • Français

The mechanism of cell membrane repair

Published online by Cambridge University Press:  16 July 2018

Tatsuru Togo ,
Janet M. Alderton  and
Richard A. Steinhardt
Tatsuru Togo
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA
Janet M. Alderton
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA
Richard A. Steinhardt
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA
Get access Rights & Permissions [Opens in a new window]

Extract

Disruption of plasma membranes is a widespread, common and normal event that occurs in many mechanically challenged tissues (McNeil & Steinhardt, 1997). After injury to the plasma membrane, rapid resealing of the membrane occurs with little loss of intracellular contents.

Analysis of plasma membrane repair in the sea urchin egg and early embryos revealed a new model of the mechanism for plasma membrane repair. Resealing of disrupted plasma membranes required external Ca2+ that could be antagonised by Mg2+. Block of Ca2+/calmodulin kinase II, which regulates exocytotic vesicle availability at synapses (Llinás et al., 1991), inhibited membrane resealing. Resealing was also inhibited by botulinum neurotoxins A, B, C1, and tetanus toxin, which disrupt SNARE vesicle docking/fusion proteins. Confocal microscopic observations of exocytotic events in sea urchin eggs and embryos during membrane resealing showed that inhibition of kinesin or myosin motor activity, which are believed to be required for vesicle transport (Goodson et al., 1997), also inhibited membrane resealing and delivery of vesicles to sites of membrane disruption. This pattern of inhibition indicates that membrane repair of micrometre-sized lesions requires vesicle delivery, docking and fusion, similar to the exocytosis of neurotransmitter (Steinhardt et al., 1994; Bi et al., 1995, 1997).

The mechanism of resealing in eggs and embyros was found to be a general property of all cells (Steinhardt et al., 1994; Togo et al., 1999). It is now known that elevated intracellular Ca2+ triggers exocytosis in various types of cells (Dan & Poo, 1992; Coorssen et al., 1996), and that endosomal compartments such as lysosomes can behave as Ca2+-regulated exocytotic vesicles (Rodríguez et al., 1997).

Type
Special Lecture for Citizens
Information
Zygote , Volume 8 , supplement S1: Proceedings of the International Symposium on Fertilization and Development of Sea Urchin and Marine Invertebrates , December 1999 , pp. S31 - S32
Copyright
Copyright © Cambridge University Press 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bi, G.-Q., Alderton, J.M. & Steinhardt, R.A. (1995). J. Cell Biol. 131, 1747–58.CrossRefGoogle Scholar
Bi, G.-Q., Morris, R.L., Liao, G., Alderton, J.M., Scholey, J.M. & Steinhardt, R.A. (1997). J. Cell Biol. 138, 999–1008.CrossRefGoogle Scholar
Coorssen, J.R., Schmitt, H. & Almers, W. (1996). EMBO J. 15, 3787–91.Google Scholar
Dan, Y. & Poo, M. (1992). Nature 359, 733–6.Google Scholar
Goodson, H.V., Valetti, C. & Kreis, T.E. (1997). Curr. Opin. Cell Biol. 9, 18–28.Google Scholar
Klausner, R.D., Donaldson, J.G. & Lippincott-Schwartz, J. (1992). J. Cell Biol. 116, 1071–80.Google Scholar
Llinás, R., Graner, J.A., Sugimori, M., McGuinness, T.L. & Greengard, P. (1991). J. Physiol, (Lond.) 436, 257–82.Google Scholar
McNeil, P.L. & Steinhardt, R.A. (1997). J. Cell Biol. 137, 1–4.Google Scholar
Rodríguez, A., Webster, P., Ortego, J. & Andrews, N.W. (1997). J. Cell Biol. 137, 93–104.CrossRefGoogle Scholar
Steinhardt, R.A., Bi, G.-Q. & Alderton, J.M. (1994). Science 263, 390–3.Google Scholar
Togo, T., Alderton, J.M., Bi, G.-Q. & Steinhardt, R.A. (1999). J. Cell Sci. 112, 719–31.Google Scholar
Zhelev, D.V. & Needham, D. (1993). Biochim. Biophys. Acta 1147, 89–104.Google Scholar