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Malonaldehyde formation and DNA fragmentation: two independent sperm decays linked to reactive oxygen species

Published online by Cambridge University Press:  24 March 2010

Debbie Montjean ,
Yves Ménézo ,
Moncef Benkhalifa ,
Marc Cohen ,
Stephanie Belloc ,
Paul Cohen-Bacrie  and
Jacques de Mouzon
Debbie Montjean
Affiliation:
ATL, R & D, Rue Louis Lormand, 78320 La Verriere, France. Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France. Clinique Natecia, Lyon, France. Human Development Genetics, Institut Pasteur, Paris, France. EA1533 Reproduction Humaine: Génétique et Thérapeutique, Université Paris VI, France.
Yves Ménézo*
Affiliation:
Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France. Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France.
Moncef Benkhalifa
Affiliation:
ATL, R & D, Rue Louis Lormand, 78320 La Verriere, France. Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France.
Marc Cohen
Affiliation:
Clinique Natecia, Lyon, France.
Stephanie Belloc
Affiliation:
Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France.
Paul Cohen-Bacrie
Affiliation:
Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France.
Jacques de Mouzon
Affiliation:
Unité INSERM 822, 82 rue Général Leclerc, 94276 Le Kremlin Bicetre, France.
*
All correspondence to: Yves Ménézo. Laboratoire d'Eylau, 55 rue St Didier, 75116 Paris, France. e-mail: [email protected]
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Summary

Malondialdehyde (MDA), a product involved in membrane lipid peroxidation, was dosed in the sperm of 163 patients who had consulted the clinic regarding hypofertility. We attempted to determine if there was correlation between MDA content, sperm World Health Organization parameters and DNA fragmentation that results mainly from reactive oxygen species assaults. We found that no correlation could be established; however MDA and sperm decondensation were shown to be significantly linked. The impact of membrane polyunsaturated fatty acids and the role of phospholipid hydroperoxide glutathione peroxidase are discussed.

Keywords

DNA fragmentationFatty acidsLipid peroxidationMalondialdehydeReactive oxygen species
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 265 - 268
Copyright
Copyright © Cambridge University Press 2010

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