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lncRNA FOXD2-AS1 affects trophoblast cell proliferation, invasion and migration through targeting miRNA

Published online by Cambridge University Press:  13 January 2020

Yulei Zhang  and
Xiaoqin Chen Open the ORCID record for Xiaoqin Chen [Opens in a new window]
Xiaoqin Chen*
Affiliation:
Obstetrics Department, Huai’an First People’s Hospital, Huaian, Jiangsu, 223300, China
*
Author for correspondence: Xiaoqin Chen. Huai’an First People’s Hospital, 1 Hunaghe West Road, Huaian, Jiangsu, 223300, China. E-mail: [email protected]
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Summary

The abnormal expression of lncRNAs and miRNAs has been found in the placentas of patients with preeclampsia (PE). Therefore, we determined the role of lncRNA FOXD2-AS1/miR-3127 in trophoblast cells. The expression of lncRNA FOXD2-AS1 was detected by qRT-PCR. The proliferation, migration and invasion ability of trophoblast cells were evaluated using CCK-8, wound healing and transwell assays. The target gene of lncRNA FOXD2-AS1 was determined by StarBase and luciferase reporter assays. Western blotting was used to analyze the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9). The results showed that FOXD2-AS1 affected trophoblast cell viability in vitro, while the expression of miR-3127 was decreased. FOXD2-AS1 silencing decreased the promotion effects on trophoblast cell induced by miR-3127 inhibition. In addition, FOXD2-AS1 and miR-3127 presented the same effect on MMP2 and MMP9 levels. lncRNA FOXD2-AS1 modulated trophoblast cell proliferation, invasion and migration through downregulating miR-3127 expression. Therefore, lncRNA FOXD2-AS1 could act as a latent therapeutic marker in preeclampsia.

Keywords

InvasionlncRNA FOXD2-AS1MigrationmiR-3127Proliferation
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 131 - 138
Copyright
© Cambridge University Press 2020

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References

Apicella, C, Ruano, CSM, Mehats, C, Miralles, F and Vaiman, D (2019) The role of epigenetics in placental development and the etiology of preeclampsia. Int J Mol Sci 20, 2837.CrossRefGoogle ScholarPubMed
Ashton, SV, Whitley, GS, Dash, PR, Wareling, M, Crocker, IP, Baker, PN and Cartwright, JE (2005) Uterine spiral artery remodeling involves endothelial apoptosis induced by extravillous trophoblasts through Fas/FasL interactions. Arterioscler Thromb Vasc Biol 25, 102–8.CrossRefGoogle ScholarPubMed
Brosens, I and Renaer, M (1972) On the pathogenesis of placental infarcts in pre-eclampsia. J Obstet Gynaecol Br Commonw 79, 794–9.CrossRefGoogle ScholarPubMed
Cao, L, Wang, Y, Wang, Q and Huang, J (2018) LncRNA FOXD2-AS1 regulates chondrocyte proliferation in osteoarthritis by acting as a sponge of miR-206 to modulate CCND1 expression. Biomed Pharmacother 106, 1220–6.CrossRefGoogle ScholarPubMed
Corthorn, J, Rey, S, Chacon, C and Valdés, G (2007) Spatio-temporal expression of MMP-2, MMP-9 and tissue kallikrein in uteroplacental units of the pregnant guinea-pig (Cavia porcellus). Reprod Biol Endocrinol 5, 27.CrossRefGoogle Scholar
Eleuterio, NM, Palei, AC, Rangel Machado, JS, Tanus-Santos, JE, Cavalli, RC and Sandrim, VC (2015) Positive correlations between circulating adiponectin and MMP2 in preeclampsia pregnant. Pregnancy Hypertens 5, 205–8.CrossRefGoogle ScholarPubMed
Harris, LK, Keogh, RJ, Wareing, M, Baker, PN, Cartwright, JE, Aplin, JD and Whitley, GS (2006) Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a Fas ligand-dependent mechanism. Am J Pathol 169, 1863–74.CrossRefGoogle ScholarPubMed
Janani, F and Changaee, F (2017) Seasonal variation in the prevalence of preeclampsia. J Family Med Prim Care 6, 766–9.Google ScholarPubMed
Jiang, J, Zhang, Y, Guo, Y, Yu, C, Chen, M, Li, Z, Tian, S and Sun, C (2015) MicroRNA-3127 promotes cell proliferation and tumorigenicity in hepatocellular carcinoma by disrupting of PI3K/AKT negative regulation. Oncotarget 6, 6359–72.Google ScholarPubMed
Li, JH, Liu, S, Zhou, H, Qu, LH and Yang, JH (2014) starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res 42, D92–7.CrossRefGoogle ScholarPubMed
Li, S, Zhai, J, Liu, J, Hong, Y, Zhao, W, Zhao, A, Sun, K, Du, Y and Chen, ZJ (2017) BMAL1 facilitates trophoblast migration and invasion via SP1-DNMT1/DAB2IP pathway in recurrent spontaneous abortion. Oncotarget 8, 89451–64.Google ScholarPubMed
Meekins, JW, Pijnenborg, R, Hanssens, M, McFadyen, IR and van Asshe, A (1994) A study of placental bed spiral arteries and trophoblast invasion in normal and severe pre-eclamptic pregnancies. Br J Obstet Gynaecol 101, 669–74.CrossRefGoogle ScholarPubMed
Rahat, B, Sharma, R, Bagga, R, Hamid, A and Kaur, J (2016) Imbalance between matrix metalloproteinases and their tissue inhibitors in preeclampsia and gestational trophoblastic diseases. Reproduction 152, 11–22.CrossRefGoogle ScholarPubMed
Soundararajan, R and Rao, AJ (2004) Trophoblast ‘pseudo-tumorigenesis’: significance and contributory factors. Reprod Biol Endocrinol 2, 15.CrossRefGoogle ScholarPubMed
Sui, C, Dong, Z, Yang, C, Zhang, M, Dai, B, Geng, L, Lu, J, Yang, J and Xu, M (2019) LncRNA FOXD2-AS1 as a competitive endogenous RNA against miR-150-5p reverses resistance to sorafenib in hepatocellular carcinoma. J Cell Mol Med 23, 6024–33.CrossRefGoogle ScholarPubMed
Sun, Y, Chen, C, Zhang, P, Xie, H, Hou, L, Hui, Z, Xu, Y, Du, Q, Zhou, X, Su, B and Gao, W (2014) Reduced miR-3127-5p expression promotes NSCLC proliferation/invasion and contributes to dasatinib sensitivity via the c-Abl/Ras/ERK pathway. Sci Rep 4, 6527.CrossRefGoogle ScholarPubMed
Tangeras, LH, Austdal, M, Skrastad, RB, Salvesen, KA, Austqulen, R, Bathen, TF and Iversen, AC (2015) Distinct first trimester cytokine profiles for gestational hypertension and preeclampsia. Arterioscler Thromb Vasc Biol 35, 2478–85.CrossRefGoogle ScholarPubMed
Wang, KC and Chang, HY (2011) Molecular mechanisms of long noncoding RNAs. Mol Cell 43, 904–14.CrossRefGoogle ScholarPubMed
Wu, D, Yang, N, Xu, Y, Wang, S, Zhang, Y, Sagnelli, M, Hu, B, Huang, Z and Sun, L (2019) lncRNA HIF1A antisense RNA 2 modulates trophoblast cell invasion and proliferation through upregulating PHLDA1 expression. Mol Ther Nucleic Acids 16, 605–15.CrossRefGoogle ScholarPubMed
Xu, Y, Lian, Y, Zhang, Y, Huang, S, Zuo, Q, Yang, N, Chen, Y, Wu, D and Sun, L (2018a) The long non-coding RNA PVT1 represses ANGPTL4 transcription through binding with EZH2 in trophoblast cell. J Cell Mol Med 22, 1272–82.Google Scholar
Xu, Y, Wu, D, Liu, J, Huang, S, Zuo, Q, Xia, X, Jiang, Y, Wang, S, Chen, Y, Wang, T and Sun, L (2018b) Downregulated lncRNA HOXA11-AS affects trophoblast cell proliferation and migration by regulating RND3 and HOXA7 expression in PE. Mol Ther Nucleic Acids 12, 195–206.CrossRefGoogle ScholarPubMed
Yang, Y, Sun, Y, Wu, Y, Tang, D, Ding, X, Xu, W, Su, B and Gao, W (2018) Downregulation of miR-3127-5p promotes epithelial-mesenchymal transition via FZD4 regulation of Wnt/beta-catenin signaling in non-small-cell lung cancer. Mol Carcinog 57, 842–53.CrossRefGoogle ScholarPubMed
Zhao, L, Zhao, Y, He, Y, Li, Q and Mau, Y (2018) The functional pathway analysis and clinical significance of miR-20a and its related lncRNAs in breast cancer. Cell Signal 51, 152–65.CrossRefGoogle ScholarPubMed