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Isolation of viable egg cells of rape (Brassica napus L.)

Published online by Cambridge University Press:  26 September 2008

Norio Katoh ,
Horst Lörz  and
Erhard Kranz
Norio Katoh
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
Horst Lörz
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
Erhard Kranz*
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
*
Dr.Erhard Kranz, Centre for Applied Plant Molecular Biology (AMP II), University of Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany.
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Summary

The isolation of viable egg cells of rape (Brassica napus L.) has been achieved from microdissected ovules. The non-gametic cells of the embryo sac, synergids and central cells have also been isolated. Their morphology corresponded to that of these cells in situ, making a discrimination from isolated sporophytic cells possible. Two hours after isolation the egg cells were still viable. Viable egg cells have been reproducibly isolated with afrequency of 25% per dissected ovule.

Keywords

Egg cell isolationEmbryo sacBrassica
Type
Article
Information
Zygote , Volume 5 , Issue 1 , February 1997 , pp. 31 - 33
Copyright
Copyright © Cambridge University Press 1997

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References

Chaboud, A.& Perez, R. (1992). Generative cells and male gametes: isolation, Physiology, and biochemistry. Int. Rev. Cytol. 140, 205–32.Google Scholar
Faure, J.-E., Mogensen, H.L., Kranz, E., Digonnet, C. & Dumas, C. (1992). Ultrastructural characterization and three-dimensional reconstruction of isolated maize(Zea mays L.) egg cell protoplasts. Protoplasma. 171, 97–103.CrossRefGoogle Scholar
Heslop-Harrison, J.& Heslop-Harrison, Y. (1970). Evaluation of pollen viability by enzymatically-induced fluorescence: intracellular hydrolysis of fluorescein diacetate. Stain Technol. 45, 115–20.CrossRefGoogle ScholarPubMed
Holm, P.B., Knudsen, S., Mouritzen, P., Negri, D., Olsen, F.L. & Roué, C. (1994). Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell. Plant Cell. 6, 531–43.CrossRefGoogle ScholarPubMed
Huang, B.-Q. & Russell, S.D. (1992). Female germ unit: organization, isolation, and function. Int. Rev. Cytol. 140, 233–93.Google Scholar
Koop, H.-U. & Schweiger, H.-G. (1985). Regeneration of plants from individually, cultived protoplasts using an in improved microculture system. J. Plant Physiol. 121, 245–57.CrossRefGoogle Scholar
Kovàcs, M., Barnabàs, B., Kranz, E. (1994). The isolation of viable egg cells of wheat (Triticum aestivum L.). Sex. Plant Reprod. 7, 311–12.Google Scholar
Kranz, E., Vonwiegen, P. & Lörz, H. (1995). Early cytological events after induction of cell division in egg cells and zygote development following in vitro fertilization with angiosperm gametes. Plant. J. 8, 9–23.CrossRefGoogle Scholar
Sumner, M.J. & Van Caseele, L. (1989). The ultrastucture and cytochemistry of the egg apparatus of Brassica campestris. Can. J. Bot. 7, 177–190.CrossRefGoogle Scholar
Theunis, C.H., Pierson, E.S. & Cresti, M. (1991). Isolation of male and female gametes in higher Plants. Sex. Plant Reprod. 4, 145–54.CrossRefGoogle Scholar
Van derMass, H.M., Zaal, M.A.C.M., De Jong, E.R., Krens, F.A. & Van Went, J.L. (1993). Isolation of viable egg cells of perennial ryegrass (Lolioum perenne L.). Protoplasma. 173, 86–9.CrossRefGoogle Scholar
Van Went, J. & Cresti, M. (1988). pre-fertilization degeneration of both synergids in Brassica campestris ovules. Sex. Plant Reprod. 1, 208–16.CrossRefGoogle Scholar
Willemse, M.T.M. & Van Went, J.L. (1984). The female gametophyte. In Embryology of Angiosperms, ed. Johri, B.M.159–96. Springer: Berlin.CrossRefGoogle Scholar
Young, B.A., Sherwood, R.T. & Bashaw, E.C. (1979). Cleared-pistil and thick-sectioning techiques for detecting aposporous apomixis in grasses. Can. J. Bot. 57, 1668–72.Google Scholar
Zhou, C. & Yang, H.Y. (1982). Enzymatic isolation of embryo sacs in angiosperms: isolation and microscopical observations of fixed materials. Acta Bot. Sin. 24, 403–7.Google Scholar
Zhou, C. & Yang, H.Y. (1982). Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species. Planta. 165, 225–31.CrossRefGoogle Scholar