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Involvement of GABAA receptor in Bufo arenarum oocyte maturation

Published online by Cambridge University Press:  01 May 2008

G. Sánchez Toranzo ,
L. Zelarayán ,
F. Bonilla ,
J. Oterino  and
M.I. Bühler
G. Sánchez Toranzo
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina.
L. Zelarayán
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina.
F. Bonilla
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina.
J. Oterino
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina.
M.I. Bühler*
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina. Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina.
*
All correspondence to: Marta I. Bühler. Departamento de Biología del Desarrollo, Chacabuco 461,4000 – San Miguel de Tucumán. Argentina. Fax: +54 381 4248025. e-mail: mbuhler@fbqf:unt.edu.ar
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Summary

Amphibian oocytes meiotic arrest is released under the stimulus of progesterone; this hormone interacts with the oocyte surface and starts a cascade of events leading to the activation of a cytoplasmic maturation promoting factor (MPF) that induces germinal vesicle breakdown (GVBD), chromosome condensation and extrusion of the first polar body.

The aim of this work was to determine whether the activation of a GABAA receptor is able to induce GVBD in fully grown denuded oocytes of Bufo arenarum and to analyse its possible participation in progesterone-induced maturation. We also evaluated the role of purines and phospholipids in the maturation process induced by a GABAA receptor agonist such as muscimol.

Our results indicated that the activation of the GABAA receptor by muscimol induces maturation in a dose- and time-dependent manner and that this activation is a genuine maturation that enables oocytes to form pronuclei. Assays with a receptor antagonist, picrotoxine, showed that the maturation induced by muscimol was inhibited. Treatment with picrotoxine, however, shows that the participation of GABAA receptor in progesterone-induced maturation is not significant.

In addition, our results indicate that high intracellular levels of purines obtained by the use of db-AMPc and theophylline or the inhibition of the phosphatidylinositol 4,5-bisphosphate (PIP2 hydrolysis by neomycin and PIP2 turn over by LiCl, respectively, inhibited the maturation induced by muscimol. Treatment with H-7 indicated, however, that PKC activation is not necessary for GVBD induced by the GABAA receptor agonist. Results suggest that the transduction pathway used by the GABAA receptor to induce maturation is different from those used by progesterone.

Keywords

Bufo arenarumGABAA receptorOocyte maturation
Type
Research Article
Information
Zygote , Volume 16 , Issue 2 , May 2008 , pp. 135 - 144
Copyright
Copyright © Cambridge University Press 2008

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