Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-13T01:03:19.575Z Has data issue: false hasContentIssue false
  • English
  • Français

Intracytoplasmic sperm injection in dysmorphic human oocytes

Published online by Cambridge University Press:  26 September 2008

Mina Alikani ,
Gianpiero Palermo ,
Alexis Adler ,
Massimo Bertoil ,
Marlena Blake  and
Jacques Cohen
Mina Alikani*
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Gianpiero Palermo
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Alexis Adler
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Massimo Bertoil
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Marlena Blake
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
Jacques Cohen
Affiliation:
The Institute for Reproductive Medicine and Science, West Orange, New Jersey; The Center for Reproductive Medicine and Infertility, New York Hospital; Department of Obstetrics and Gynecology, New york University; and Department of Obstetrics and Gynecology, Parma University, Italy
*
Mina Alikani, The Institute for Reproductive Medicine and Science, Saint Barbabas Medical Center, 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052, USA. Telephone: 201–234–6237. Fax: 201–234–6235.
Get access Rights & Permissions [Opens in a new window]

Summary

Fertilisation and development of dysmorphic human oocytes recovered from hyperstimulated ovaries have been evaluated following intracytoplasmic sperm injection (ICSI) for treatment of male infertility. A total of 2968 oocytes at metaphase II of meiosis were injected, of which 806 (27.2%) were dysmorphic at the light microscopic level. Cytoplasmic abnormalities included granularity, areas of necrosis, organelle clustering, vacuoles, and accumulating saccules of smooth endoplasmic reticulum. Anomalies of the first polar body and zona pellucida, as well as non-spherical shapes of oocytes, were also noted. Contrary to previous findings linking some dysmorphisms to non-assisted fertilisation failure, in this study no single abnormality led to a reduction in the fertilisation rate, nor was fertilisation compromised in oocytes with multiple abnormalities. The incidence of normal fertilisation (two pronuclei and two polar bodies) was 69% in both the dysmorphic and non-dysmorphic oocytes. While overall pregnancy and implantation results were not altered in the group of patients (n = 242) in whom at least one dysmorphic oocyte was injected, exclusive replacement of embryos which originated from dysmorphic oocytes led to a higher incidence of early pregnancy loss. It is concluded that aberrations in the morphology of human oocytes – most probably a product of controlled ovarian stimulation – are of little or no consequence to fertilisation or early cleavage after ICSI. It is possible, however, that these embryos have a reduced potential for implantation and further development.

Keywords

DysmorphicICSIImplantationOocytePregnancy loss
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 283 - 288
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Almeida, P.A. & Bolton, V. (1993). Immaturity and chromosomal abnormalities in oocytes that fail to develop pronuclei following insemination in vitro. Hum. Reprod. 8, 229–32.CrossRefGoogle ScholarPubMed
Angell, R.R., Ledger, W., Yong, E.L., Harkness, L. & Baird, D.T. (1991). Cytogenetic analysis of unfertilized human oocytes. Hum. Reprod. 6, 568–73.CrossRefGoogle ScholarPubMed
Bedford, J.M. & Kim, H.H. (1993). Sperm/egg binding patterns and oocyte cytology in retrospective analysis of fertilization failure in vitro. Hum.Reprod. 8, 453–63.CrossRefGoogle ScholarPubMed
Bongso, A., Soon Chye, N., Ratnam, S., Sathananthan, H. & Wong, P.C. (1988). Chromosome anomalies in human oocytes failing to fertilize after insemination in vitro. Hum. Reprod. 3, 645–9.CrossRefGoogle ScholarPubMed
Cohen, J. (1992). A review of clinical microsurgical fertilization. In Micromanipulation of Human Gametes and Embryos, by Cohen, J., Malter, H.Talansky, B.E. & Grifo, J., pp. 163–90. New York: Raven Press.Google ScholarPubMed
Lindenberg, S., Hyttel, P., Lenz, S. & Holmes, P.V. (1986). Ultrastructure of the early human implantation in vitro. Hum. Reprod. 1, 533–8.CrossRefGoogle ScholarPubMed
Nayudu, P.L., Lopata, A., Jones, G.M., Cook, D.A., Bourne, H.M., Sheather, S.J., Brown, T.C. & Johnston, W.I.H. (1989). An analysis of human oocytes and follicles from stimulated cycles: oocyte morphology and associated follicular fluid characteristics. Hum. Reprod. 4, 558–67.CrossRefGoogle ScholarPubMed
Palermo, G., Joris, H., Devroey, P. & Van Steirteghem, A.C. (1992). Pregnancies after intracytoplasmic injection of a single spermatozoon into an oocyte. Lancet 340, 17–18.CrossRefGoogle ScholarPubMed
Palermo, G., Cohen, J., Alikani, M., Adler, A. & Rosenwaks, Z. (1995). Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil. Steril. 63, 1231–40.CrossRefGoogle ScholarPubMed
Plachot, M., Veiga, A., Montagut, J., de Grouchy, J., Calderon, G., Lepretre, S., Junca, A., Santalo, J., Carles, E., Mandelbaum, J., Barri, P., Degoy, J., Cohen, J., Egozcue, J., Sabatier, J.C. & Salat-Baroux, J. (1988). Are clinical and biological IVF parameters correlated with chromosomal disorders in early life? A multicentric study. Hum. Reprod. 3, 627–35.CrossRefGoogle ScholarPubMed
Van Blerkom, J. (1990a). Extrinsic and intrinsic influences on human oocyte and early embryonic development potential. In Elements of Mammalian Fertilization, ed. Wasserman, P.M. pp. 81–109. Boca Raton, FL: pp. 81–109.Google Scholar
Van Blerkom, J. (1990b). Occurrence and developmental consequences of aberrant cellular organization in meiotically mature human oocytes after exogenous ovarian hyperstimulation. J. Electron Microsc. Tech. 16, 324–6.CrossRefGoogle ScholarPubMed
Van Blerkom, J. & Henry, G. (1992). Oocyte dysmorphism and aneuploidy in meiotically mature oocytes after ovarian stimulation. Hum. Reprod. 7, 379–90.CrossRefGoogle ScholarPubMed
Veeck, L. (1988). Oocyte assessment and biological performance. Ann. NY Acad. Sci. 541, 259–74.CrossRefGoogle ScholarPubMed
Warburton, D., Byrne, J. & Canki, N. (1991). Chromosome Anomalies and Prenatal Development: An Atlas. New York: Oxford University Press.Google Scholar
West, J.D. (1993). A genetically defined animal model of anembryonic pregnancy. Hum. Reprod. 8, 1316–22.CrossRefGoogle ScholarPubMed