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Insulin-transferrin-selenium supplementation improves porcine embryo production in vitro

Published online by Cambridge University Press:  25 November 2024

Juan Lin Open the ORCID record for Juan Lin [Opens in a new window] ,
Zhuqing Ji  and
Shenming Zeng Open the ORCID record for Shenming Zeng [Opens in a new window]
Juan Lin
Affiliation:
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Rural Area, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China
Zhuqing Ji
Affiliation:
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Rural Area, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China
Shenming Zeng*
Affiliation:
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Rural Area, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China
*
Corresponding author: Shenming Zeng; Email: [email protected]
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Summary

In vitro production of porcine embryos is a complicated process that includes in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). Insufficient cytoplasmic maturation, slow zona reaction and improper embryo culture conditions will compromise the efficiency of porcine embryo production in vitro. Previous studies have shown that insulin-transferrin-selenium (ITS) in IVM or IVC medium could improve porcine oocyte maturation, decrease polyspermy fertilization and promote subsequent embryonic development in vitro. However, the effect of ITS both in IVM and IVC media on porcine embryo production in vitro hasn’t been elucidated. In this study, we found that 1.0% ITS supplementation in IVM/IVC media promoted the expansion of cumulus cells, raised mitochondrial membrane potential, increased ATP content and reduced ROS level in matured oocytes, improved blastocyst rate and the cell number of blastocyst, simultaneously. In conclusion, the IVM/IVC media supplemented with 1.0% ITS can improve the efficiency of porcine embryo production in vitro.

Keywords

Embryo production in vitroinsulin-transferrin-seleniummitochondrial functionoocyteporcine
Type
Research Article
Information
Zygote , Volume 32 , Issue 6 , December 2024 , pp. 452 - 458
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

†

These authors contributed equally to this work.

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