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Induction of DNA replication in germinal vesicles and in nuclei formed in maturing mouse oocytes by 6-DMAP treatment

Published online by Cambridge University Press:  26 September 2008

J. Fulka Jr ,
N.L. First ,
C. Lee ,
J. Fulka  and
R.M. Moor
J. Fulka Jr*
Affiliation:
Institute of Animal Production, Prague, Czech Republic, The Babraham Institute, Cambridge, UK, and Department of Meat and Animal Science, University of Wisconsin, USA
N.L. First
Affiliation:
Institute of Animal Production, Prague, Czech Republic, The Babraham Institute, Cambridge, UK, and Department of Meat and Animal Science, University of Wisconsin, USA
C. Lee
Affiliation:
Institute of Animal Production, Prague, Czech Republic, The Babraham Institute, Cambridge, UK, and Department of Meat and Animal Science, University of Wisconsin, USA
J. Fulka*
Affiliation:
Institute of Animal Production, Prague, Czech Republic, The Babraham Institute, Cambridge, UK, and Department of Meat and Animal Science, University of Wisconsin, USA
R.M. Moor
Affiliation:
Institute of Animal Production, Prague, Czech Republic, The Babraham Institute, Cambridge, UK, and Department of Meat and Animal Science, University of Wisconsin, USA
*
J. Fulka, Jr, Institute of Animal Production, CS-104 01 Prague 10, Czech Republic. Tel: +42 2 67710659. Fax: +42 2 67710799.
J. Fulka, Jr, Institute of Animal Production, CS-104 01 Prague 10, Czech Republic. Tel: +42 2 67710659. Fax: +42 2 67710799.
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Summary

Immature mouse oocytes (germinal vesicle stage, GV), oocytes at different stages during maturation (prometaphase to anaphase I) and matured oocytes (metaphase II arrested) were cultured in 6-dimethylaminopurine (6-DMAP)-supplemented medium also containing bromodeoxyuridine for the assessment of DNA replication in these cells. Immature oocytes remained arrested at the GV stage and DNA replication was never detected in them. On the other hand, oocytes at the prometaphase to anaphase-telophase I stages responded to 6-DMAP treatment by forming nuclei which synthesised DNA. Mature (metaphase II) oocytes did not respond to 6-DMAP and their chromatin remained condensed. DNA synthesis could even be induced in GV-staged oocytes, but only when they were fused to freshly activated oocytes and incubated in 6-DMAP-supplemented medium.

Keywords

6-DMAPDNA replicationOocytes
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 213 - 217
Copyright
Copyright © Cambridge University Press 1997

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