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In vivo storage of XR family interspersed RNA in Xenopus oocytes

Published online by Cambridge University Press:  26 September 2008

Chengyu Liu  and
L. Dennis Smith
Chengyu Liu*
Affiliation:
Department of Developmental and Cell Biology and Developmental Biology Center, University of California at Irvine, Irvine, California, USA
L. Dennis Smith
Affiliation:
Department of Developmental and Cell Biology and Developmental Biology Center, University of California at Irvine, Irvine, California, USA
*
1 Dr Chengyu liu, Roche Institute of Molecular Biology, 340 Kingsland Street, Nutley, NJ 07110, USA. Telephone: (201)-235-4574. Fax: (201)-235-2839.
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Summary

Interspersed RNA is an abundant class of cytoplasmic poly(A)+ RNA which contains repetitive elements within mostly heterogeneous single copy sequences. In spite of its quantitative importance in oocytes or eggs (two-thirds of the total poly(A)+ RNA), very little is known about its synthesis, its interaction with other molecules, and its functional significance. Here, we analysed a prevalent family of interspersed RNa (XR family) during Xenopus oogenesis. We found that XR interspersed RNA, unlike extracted interspersed RNA, did not form RNA duplexes in vivo. Im small oocytes (stage III), XR RNA interacted with proteins forming rapidly sedimenting ribonucleoprotein particles (RNPs) with a median sedimentaion constant of 80S. However, towards the end of oogenesis (stage VI), these XR RNPs changed into smaller particles with a median sedimentaion constant of 40S. By analysing the proteins associated with XR RNA sequence, we have identified a 42 kilodalton protein in small oocytes, which was replaced by a 45 kilodalton protein at stage V of oogenesis.

Keywords

Interspersed RNARibonucleoprotein particleRNA binding proteinXenopus oocyte
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 37 - 44
Copyright
Copyright © Cambridge University Press 1995

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Footnotes

1PO Box 830745, University of Nebraska, Lincoln, Nebraska, 68583–0745., USA.

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