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In vitro culture systems as an alternative for female reproductive toxicology studies

Published online by Cambridge University Press:  15 March 2019

Denise Damasceno Guerreiro Open the ORCID record for Denise Damasceno Guerreiro [Opens in a new window] ,
Gildas Tetaping Mbemya ,
Jamily Bezerra Bruno ,
Luciana Rocha Faustino ,
José Ricardo de Figueiredo  and
Ana Paula Ribeiro Rodrigues
Denise Damasceno Guerreiro*
Affiliation:
Faculdade de Veterinária, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-Antrais (Lamofopa), Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil
Gildas Tetaping Mbemya
Affiliation:
Faculdade de Veterinária, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-Antrais (Lamofopa), Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil
Jamily Bezerra Bruno
Affiliation:
Faculdade de Veterinária, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-Antrais (Lamofopa), Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil
Luciana Rocha Faustino
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Campus Ministro Reis Velloso, Universidade Federal do Piauí, Paranaíba, Piauí, Brasil
José Ricardo de Figueiredo
Affiliation:
Faculdade de Veterinária, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-Antrais (Lamofopa), Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil
Ana Paula Ribeiro Rodrigues
Affiliation:
Faculdade de Veterinária, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-Antrais (Lamofopa), Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil
*
Address for correspondence: Denise Damasceno Guerreiro. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Dr. Silas Munguba, 1700, Campus do Itaperi, Fortaleza – CE – Brasil. CEP: 60 714 903. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. E-mail: [email protected]
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Summary

Studies have shown that daily exposure to different products, whether chemical or natural, can cause irreversible damage to women’s reproductive health. Therefore it is necessary to use tests that evaluate the safety and efficacy of these products. Most reproductive toxicology tests are performed in vivo. However, in recent years, various cell culture methods, including embryonic stem cells and tissues have been developed with the aim of reducing the use of animals in toxicological tests. This is a major advance in the area of toxicology, as these systems have the potential to become a widely used tool compared with in vivo tests routinely used in reproductive biology and toxicology. The present review describes and highlights data on in vitro culture processes used to evaluate reproductive toxicity as an alternative to traditional methods using in vivo tests.

Keywords

Alternative methodsFemale gonadOvarian functionReproductive functionToxicity
Type
Review Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 55 - 63
Copyright
© Cambridge University Press 2019 

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