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The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm–zona pellucida binding

Published online by Cambridge University Press:  15 March 2019

Juan Patricio Anchordoquy ,
Juan Mateo Anchordoquy ,
Raúl Martín Lizarraga ,
Noelia Nikoloff ,
Ana Malen Pascua  and
Cecilia Cristina Furnus Open the ORCID record for Cecilia Cristina Furnus [Opens in a new window]
Juan Patricio Anchordoquy
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina
Juan Mateo Anchordoquy
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina
Raúl Martín Lizarraga
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina
Noelia Nikoloff
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina
Ana Malen Pascua
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina
Cecilia Cristina Furnus*
Affiliation:
Igevet e Instituto de Genética Veterinaria ‘Prof. Fernando N. Dulout’ (UNLP-Conicet La Plata), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina Cátedra de Citología, Histología y Embriología ‘A’ Facultad de Ciencias Médicas, Universidad Nacional de La Plata, calle 60 y 120 s/n, CP1900, La Plata, Buenos Aires, Argentina
*
Address for correspondence: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET, UNLP-CONICET La Plata). Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata, Calle 60 y 118 s/n CP (1900), La Plata, Buenos Aires, Argentina. Tel: +54 0221 5221488. E-mail: [email protected]
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Summary

Sperm–zona pellucida (ZP) binding is a necessary event for successful fertilization. The aim of this study was to determine the effect of trace minerals such as copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) on bovine spermatozoa binding to ZP. Sperm viability, functional membrane integrity, acrosomal status (AS), total antioxidant capacity (TAC) and sperm lipid peroxidation (LPO) were also evaluated. For the present study, in vitro fertilization (IVF) medium was supplemented with Cu (0.4 µg/ml Cu), Mn (5 ng/ml Mn), Se (100 ng/ml Se), Zn (0.8 µg/ml Zn), all minerals (Cu+Mn+Se+Zn), or tested without supplement (Control). Considerably more sperm bound to ZP when Cu, Se or Zn were added to the IVF medium, but there were no difference compared with the Control, Mn and Cu+Mn+Se+Zn groups. After 1 h of incubation, viability was increased by the addition of Cu, Mn and Se with respect to the Control but, after 2 h, viability was higher only with the addition of Mn to IVF medium. Functional membrane integrity improved in sperm treated with Cu. Acrosome integrity was higher in sperm treated with Zn after 1 h of incubation. LPO was significantly higher in sperm treated with Cu or Cu+Mn+Se+Zn. The mean TACs of sperm treated with Cu, Mn, Zn or Cu+Mn+Se+Zn were lower than in the Control. In conclusion, the results obtained in the present study determined that the presence of Cu, Se and Zn in the IVF medium increased the number of spermatozoa bound to the ZP, highlighting the importance of these minerals in the fertilization process.

Keywords

Lipid peroxidationMTT assayOxidative statusSperm qualityTrace element
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 89 - 96
Copyright
© Cambridge University Press 2019 

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