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Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen–thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos

Published online by Cambridge University Press:  29 January 2010

J. Peippo ,
M. Räty ,
K. Korhonen ,
M. Eronen ,
K. Kananen ,
T. Hurme ,
M. Halmekytö  and
A. Mäki-Tanila
J. Peippo*
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
M. Räty
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
K. Korhonen
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
M. Eronen
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
K. Kananen
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
T. Hurme
Affiliation:
MTT Agrifood Research Finland, Services Unit, FI-31600 Jokioinen, Finland.
M. Halmekytö
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
A. Mäki-Tanila
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
*
All correspondence to: J. Peippo. MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland. e-mail: [email protected]
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Summary

We studied whether bovine embryos developing after in vitro fertilization (IVF) with sex-sorted spermatozoa differed in developmental kinetics, quality and sex ratio from embryos produced with unsorted spermatozoa. Abattoir-derived oocytes were fertilized with X-sorted, Y-sorted or unsorted spermatozoa from a single bull. To evaluate economical use of the sex-sorted spermatozoa, washed spermatozoa from a single straw (2 million spermatozoa) were used to fertilize each batch of collected oocytes without any further isolation steps. Concentration of the unsorted spermatozoa was adjusted accordingly. Fertilizations were assessed by staining sperm asters at 10 hpi and pronuclei at 20 hpi. Embryo development and morphological quality were monitored on days 2, 7, 8 and 9 of the development (IVF = day 0). All embryos were sexed using PCR. Following fertilization, penetration and subsequent cleavage rates were compromised in the X-sorted group compared with the Y-sorted and unsorted groups (penetration: 58.0% vs. 89.8% and 90.0%, cleavage: 65.3% vs. 81.5% and 75.0%). The use of the sex-sorted spermatozoa did not, however, reduce the proportion of transferable embryos (sex-sorted 29.6% vs. unsorted 27.7%) or their quality (quality 1: sex-sorted 36.0% vs. unsorted 19.9%). The Y-sorted spermatozoa produced more transferable embryos of better quality than the X-sorted spermatozoa (days 7–8: 31.9% vs. 26.4%, quality 1: 38.9% vs. 30.6%). On average, out of 10 transferable embryos, nine were of the predicted sex in the X- and Y-sorted spermatozoa groups. These results indicate that low numbers of X- and Y-sorted spermatozoa can be used successfully for female and male embryo production in vitro.

Keywords

CattleEmbryo sexingFlow cytometrySexual dimorphismSperm aster
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 185 - 194
Copyright
Copyright © Cambridge University Press 2010

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References

Agresti, A. (2002). Categorical Data Analysis. 2nd edn.Hoboken, New Jersey: John Wiley & Sons, Inc.CrossRefGoogle Scholar
Alomar, M., Mahieu, J., Verhaeghe, B., Defoin, L. & Donnay, I. (2006). Assessment of sperm quality parameters of six bulls showing different abilities to promote embryo development in vitro. Reprod. Fertil. Dev. 18, 395–402.CrossRefGoogle ScholarPubMed
Beyhan, Z., Johnson, L.A. & First, N.L. (1999). Sexual dimorphism in IVM–IVF bovine embryos produced from X and Y chromosome-bearing spermatozoa sorted by high speed flow cytometry. Theriogenology 52, 35–48.CrossRefGoogle ScholarPubMed
Bermejo-Alvarez, P., Rizos, D., Rath, D., Lonergan, P. & Gutiérrez-Adán, A. (2008). Can bovine in-vitro matured oocytes selectively process X- and Y-sorted sperm differentially? Biol. Reprod. 79, 594–7.CrossRefGoogle ScholarPubMed
Bredbacka, K. & Bredbacka, P. (1996). Glucose controls sex-related growth rate differences of bovine embryos produced in vitro. J. Reprod. Fertil. 106, 169–72.CrossRefGoogle ScholarPubMed
Bredbacka, P. & Peippo, J. (1992). Sex diagnosis of ovine and bovine embryos by enzymatic amplification and digestion of DNA from the ZFY/ZFX locus. Agric. Sci. Finl. 1, 233–8.Google Scholar
Collett, D. (2003). Modelling Binary Data. 2nd edn.Boca Raton, Florida: Chapman & Hall/CRC.Google Scholar
CYTEL Software Corporation. (2002). LogXact® 5–User Manual. Cambridge, MA: CYTEL Software Corporation.Google Scholar
Hasler, J.F., Cardey, E., Stokes, J.E. & Bredbacka, P. (2002). Nonelectrophoretic PCR-sexing of bovine embryos in a commercial environment. Theriogenology 58, 1457–69.CrossRefGoogle Scholar
Holm, P., Booth, P.J., Schmidt, M.H., Greve, T. & Callesen, H. (1999). High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum proteins. Theriogenology 52, 683–700.CrossRefGoogle ScholarPubMed
Kananen-Anttila, K., Lindeberg, H., Reinikainen, E., Kaimio, I., Peippo, J. & Halmekytö, M. (2005). Efficiency of donor-wise IVP using slaughtered top breeding oocyte donor cows, a retrospective summary. 21st Annual Meeting of A.E.T.E. p. 152.Google Scholar
Kawarsky, S.J., Basrur, P.K., Stubbings, R.B., Hansen, P.J. & King, W.A. (1996). Chromosomal abnormalities in bovine embryos and their influence on development. Biol. Reprod. 54, 53–9.CrossRefGoogle ScholarPubMed
Korhonen, K., Kananen, K., Ketoja, E., Matomäki, J., Halmekytö, M. & Peippo, J. (2008). Effects of serum-free in vitro maturation of bovine oocytes on subsequent embryo development and cell allocation in two developmental stages of day 7 blastocysts. Reprod. Dom. Anim. Epub ahead of print.Google Scholar
Korpiaho, P., Strandén, I. & Mäntysaari, E.A. (2003). Bull selection across age classes and variable female reproductive traits in an open nucleus breeding scheme for dairy cattle. Acta Agric. Scand., Sect. A, Animal Sci. 53, 74–82.Google Scholar
Lang, T.A., Secic, M. & Huth, E.J. (1997). How To Report Statistics in Medicine: Annotated Guidelines for Authors, Editors and Reviewers. 367 pp. Philadelphia, PA: American College of Physicians.Google Scholar
Littell, R.C., Milliken, G.A., Stroup, W.W. & Wolfinger, R.D. (1996). SAS® System for Mixed Models. 633 pp. Cary, NC: SAS Institute Inc.Google Scholar
Lopes, A.S., Larsen, L.H., Ramsign, N., Lovendahl, P., Räty, M., Peippo, J., Greve, T. & Callesen, H. (2005). Respiration rates of individual bovine IVP embryos measured with a novel, non-invasive and rapid microsensor system. Reproduction 130, 669–79.CrossRefGoogle Scholar
Lu, K.H., Cran, D.G. & Seidel, G.E. (1999). In vitro fertilization with flow-cytometrically-sorted bovine sperm. Theriogenology 52, 1393–405.CrossRefGoogle ScholarPubMed
Morton, K.M., Herrmann, D., Sieg, B., Struckmann, C., Maxwell, W.M.C., Rath, D., Evans, G., Lucas-Hahn, A., Niemann, H. & Wrenzycki, C. (2007). Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow-cytometrically sex-sorted sperm. Mol. Reprod. Dev. 74, 931–40.CrossRefGoogle ScholarPubMed
Navara, C.S., First, N.L. & Schatten, G. (1994). Microtubule organization in the cow during fertilization, polyspermy, parthenogenesis and nuclear transfer: the role of sperm aster. Dev. Biol. 162, 29–40.CrossRefGoogle ScholarPubMed
Navara, C.S., First, N.L. & Schatten, G. (1996). Phenotypic variations among paternal centrosomes expressed within the zygote as disparate microtubule lengths and sperm aster organization: correlations between centrosome activity and developmental success. Proc. Natl. Acad. Sci. USA 93, 5384–8.CrossRefGoogle ScholarPubMed
Parrish, J.J., Susko-Parrish, J.L., Winer, M.A. & First, N.L. (1988). Capacitation of bovine sperm by heparin. Biol. Reprod. 38, 1171–80.CrossRefGoogle ScholarPubMed
Parrish, J.J., Susko-Parrish, J.L. & First, N.L. (1989). Capacitation of bovine sperm by heparin: inhibitory effect of glucose and the role of intracellular pH. Biol. Reprod. 41, 683–99.CrossRefGoogle ScholarPubMed
Robertson, E. & Nelson, R. (1998). Certification and identification of the embryo. In Manual of the International Embryo Transfer Society 3rd edn. (eds Stringfellow, D.A. & Seidel, S.M.) USA.Google Scholar
Seidel, G.E. Jr (2003). Economics of selecting for sex: the most important genetic trait. Theriogenology 59, 585–98.CrossRefGoogle ScholarPubMed
Strandén, I., Korpiaho, P., Pakula, M. & Mäntysaari, E.A. (2001). Bull selection in MOET nucleus breeding schemes with limited testing capacity. Acta Agric. Scand., Sect. A, Animal Sci. 51, 235–45.Google Scholar
Thouas, G.A., Korfiatis, N.A., French, A.J., Jones, G.M. & Trounson, A.O. (2001). Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts. Reprod. BioMed. Online 3, 25–9.CrossRefGoogle ScholarPubMed
Wilson, R.D., Weigel, K.A., Fricke, P.M., Rutledge, J.J., Leibfried-Rutledge, M.L., Matthews, D.L. & Schutzkus, V.R. (2005). In vitro production of Holstein embryos using sex-sorted sperm and oocytes from selected cull cows. J. Dairy Sci. 88, 776–82.CrossRefGoogle ScholarPubMed
Wilson, R.D., Fricke, P.M., Leibfried-Rutledge, M.L., Rutledge, J.J., Syverson Penfield, C.M. & Weigel, K.A. (2006). In vitro production of bovine embryos using sex-sorted sperm. Theriogenology 65, 1007–15.CrossRefGoogle ScholarPubMed
Xu, J., Guo, Z., Su, L., Nedambale, T.L., Zhang, J., Schenk, J., Moreno, J.F., Dinnyés, A., Ji, W., Tian, X.C., Yang, X. & Du, F. (2006). Developmental potential of vitrified Holstein cattle embryos fertilized in vitro with sex-sorted sperm. J. Dairy Sci. 89, 2510–8.CrossRefGoogle ScholarPubMed
Zhang, M., Lu, K.H. & Seidel, G.E. Jr (2003). Development of bovine embryos after in vitro fertilization of oocytes with flow-cytometrically sorted, stained and unsorted sperm form different bulls. Theriogenology 60, 1657–63.CrossRefGoogle Scholar