Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-05T03:57:19.788Z Has data issue: false hasContentIssue false

Identification and expression analysis of Dazl homologue in Cynops cyanurus

Published online by Cambridge University Press:  28 July 2021

Yinjiao Zhao
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Ya Du
Affiliation:
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Qinglan Ge
Affiliation:
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Fang Yan*
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China
Shu Wei*
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China
*
Authors for correspondence: Fang Yan and Shu Wei. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China. E-mail: [email protected]; [email protected]
Authors for correspondence: Fang Yan and Shu Wei. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China. E-mail: [email protected]; [email protected]

Summary

The Dazl (deleted in azoospermia-like) gene encodes an RNA-binding protein containing an RNA recognition motif (RRM) and a DAZ motif. Dazl is essential for gametogenesis in vertebrates. In this study, we report the cloning of Dazl cDNA from Cynops cyanurus. Ccdazl mRNA showed a germline-specific expression pattern as expected. Ccdazl expression gradually decreased during oogenesis, suggesting that it may be involved in oocyte development. Phylogenetic analysis revealed that the Ccdazl protein shares conserved motifs/domains with Dazl proteins from other species. Cloning of Ccdazl provides a new tool to carry out comparative studies of germ cell development in amphibians.

Type
Research Article
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cooke, HJ, Lee, M, Kerr, S and Ruggiu, M (1996). A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads. Hum Mol Genet 5, 513–6.Google ScholarPubMed
Eberhart, CG, Maines, JZ and Wasserman, SA (1996). Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia. Nature 381(6585), 783–5.Google ScholarPubMed
Elis, S, Batellier, F, Couty, I, Balzergue, S, Martin-Magniette, ML, Monget, P, Blesbois, E and Govoroun, MS (2008). Search for the genes involved in oocyte maturation and early embryo development in the hen. BMC Genomics 9, 110.Google ScholarPubMed
Extavour, CG and Akam, M (2003). Mechanisms of germ cell specification across the metazoans: epigenesis and preformation. Development 130, 5869–84.CrossRefGoogle ScholarPubMed
Hashimoto, Y, Maegawa, S, Nagai, T, Yamaha, E, Suzuki, H, Yasuda, K and Inoue, K (2004). Localized maternal factors are required for zebrafish germ cell formation. Dev Biol 268, 152–61.CrossRefGoogle ScholarPubMed
Houston, DW and King, ML (2000). A critical role for Xdazl, a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus . Development 127, 447–56.CrossRefGoogle ScholarPubMed
Houston, DW, Zhang, J, Maines, JZ, Wasserman, SA and King, ML (1998). A Xenopus DAZ-like gene encodes an RNA component of germ plasm and is a functional homologue of Drosophila boule. Development 125, 171–80.Google ScholarPubMed
Johnson, AD, Bachvarova, RF, Drum, M and Masi, T (2001). Expression of axolotl DAZL RNA, a marker of germ plasm: widespread maternal RNA and onset of expression in germ cells approaching the gonad. Dev Biol 234, 402–15.Google ScholarPubMed
Karashima, T, Sugimoto, A and Yamamoto, M (2000). Caenorhabditis elegans homologue of the human azoospermia factor DAZ is required for oogenesis but not for spermatogenesis. Development 127, 1069–79.CrossRefGoogle Scholar
Kee, K, Angeles, VT, Flores, M, Nguyen, HN and Reijo Pera, RA (2009). Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation. Nature 462(7270), 222–5.CrossRefGoogle ScholarPubMed
Lehmann, R and Ephrussi, A (1994). Germ plasm formation and germ cell determination in Drosophila . Ciba Found Symp 182, 282–96; discussion 296–300.Google ScholarPubMed
Lin, Y and Page, DC (2005). Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice. Dev Biol 288, 309–16.CrossRefGoogle ScholarPubMed
Maegawa, S, Yasuda, K and Inoue, K (1999). Maternal mRNA localization of zebrafish DAZ-like gene. Mech Dev 81(1–2), 223–6.CrossRefGoogle ScholarPubMed
McNeilly, JR, Saunders, PT, Taggart, M, Cranfield, M, Cooke, HJ and McNeilly, AS (2000). Loss of oocytes in Dazl knockout mice results in maintained ovarian steroidogenic function but altered gonadotropin secretion in adult animals. Endocrinology 141, 4284–94.CrossRefGoogle ScholarPubMed
Picelli, S, Faridani, OR, Björklund, AK, Winberg, G, Sagasser, S and Sandberg, R (2014). Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171–81.CrossRefGoogle ScholarPubMed
Reijo, R, Lee, TY, Salo, P, Alagappan, R, Brown, LG, Rosenberg, M, Rozen, S, Jaffe, T, Straus, D, Hovatta, O, de la Chapelle, A, Silber, S and Page, DC (1995). Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nat Genet 10, 383–93.Google ScholarPubMed
Reijo, R, Seligman, J, Dinulos, MB, Jaffe, T, Brown, LG, Disteche, CM and Page, DC (1996). Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty. Genomics 35, 346–52.Google ScholarPubMed
Reynolds, N, Collier, B, Bingham, V, Gray, NK and Cooke, HJ (2007). Translation of the synaptonemal complex component Sycp3 is enhanced in vivo by the germ cell specific regulator Dazl. RNA 13, 974–81.Google ScholarPubMed
Reynolds, N, Collier, B, Maratou, K, Bingham, V, Speed, RM, Taggart, M, Semple, CA, Gray, NK and Cooke, HJ (2005). Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells. Hum Mol Genet 14, 3899–909.Google ScholarPubMed
Ruggiu, M, Speed, R, Taggart, M, McKay, SJ, Kilanowski, F, Saunders, P, Dorin, J and Cooke, HJ (1997). The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature 389(6646), 73–7.CrossRefGoogle ScholarPubMed
Saitou, N and Nei, M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–25.Google ScholarPubMed
Schrans-Stassen, BH, Saunders, PT, Cooke, HJ and de Rooij, DG (2001). Nature of the spermatogenic arrest in Dazl–/– mice. Biol Reprod 65, 771–6.Google ScholarPubMed
Sekizaki, H, Takahashi, S, Tanegashima, K, Onuma, Y, Haramoto, Y and Asashima, M (2004). Tracing of Xenopus tropicalis germ plasm and presumptive primordial germ cells with the Xenopus tropicalis DAZ-like gene. Dev Dyn 229, 367–72.CrossRefGoogle ScholarPubMed
Shah, C, Vangompel, MJ, Naeem, V, Chen, Y, Lee, T, Angeloni, N, Wang, Y and Xu, EY (2010). Widespread presence of human BOULE homologs among animals and conservation of their ancient reproductive function. PLoS Genet 6, e1001022.Google ScholarPubMed
Smith, LD (1966). The role of a “germinal plasm” in the formation of primordial germ cells in Rana pipiens . Dev Biol 14, 330–47.CrossRefGoogle ScholarPubMed
Stothard, P (2000). The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. BioTechniques 28, 1102, 1104.CrossRefGoogle ScholarPubMed
Swiers, G, Chen, YH, Johnson, AD and Loose, M (2010). A conserved mechanism for vertebrate mesoderm specification in urodele amphibians and mammals. Dev Biol 343(1–2), 138–52.CrossRefGoogle ScholarPubMed
Takeda, Y, Mishima, Y, Fujiwara, T, Sakamoto, H and Inoue, K (2009). DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish. PLoS ONE 4, e7513.Google ScholarPubMed
Tamori, Y, Iwai, T, Mita, K and Wakahara, M (2004). Spatio-temporal expression of a DAZ-like gene in the Japanese newt Cynops pyrrhogaster that has no germ plasm. Dev Genes Evol 214, 615–27.Google ScholarPubMed
Teng, YN, Lin, YM, Lin, YH, Tsao, SY, Hsu, CC, Lin, SJ, Tsai, WC and Kuo, PL (2002). Association of a single-nucleotide polymorphism of the deleted-in-azoospermia-like gene with susceptibility to spermatogenic failure. J Clin Endocrinol Metab 87, 5258–64.CrossRefGoogle ScholarPubMed
Wang, C, Fei, L and Ye, C (1984). A preliminary observation on the early development of Cynops cyanurus . Chin J Zool 19, 48.Google Scholar
Yen, PH, Chai, NN and Salido, EC (1996). The human autosomal gene DAZLA: testis specificity and a candidate for male infertility. Hum Mol Genet 5, 2013–7.CrossRefGoogle Scholar
Yu, Z, Ji, P, Cao, J, Zhu, S, Li, Y, Zheng, L, Chen, X and Feng, L (2009). Dazl promotes germ cell differentiation from embryonic stem cells. J Mol Cell Biol 1, 93103.CrossRefGoogle ScholarPubMed