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Glycerol decreases the integrity of the perinuclear theca in boar sperm

Published online by Cambridge University Press:  05 April 2012

Marco Antonio Arenas Núñez ,
María de Lourdes Juárez-Mosqueda ,
Oscar Gutiérrez-Pérez ,
Santiago René Anzaldúa Arce ,
Alejandro Córdova Izquierdo ,
Roberto Martínez Rodríguez  and
María Elena Trujillo Ortega
Marco Antonio Arenas Núñez
Affiliation:
Departamento de Morfología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México D.F. C.P. 04510, México.
María de Lourdes Juárez-Mosqueda
Affiliation:
Departamento de Morfología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México D.F. C.P. 04510, México.
Oscar Gutiérrez-Pérez
Affiliation:
Departamento de Medicina Experimental del Instituto Nacional de Pediatría, México, México D.F. C.P. 04530, México.
Santiago René Anzaldúa Arce*
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510, México, D.F. México.
Alejandro Córdova Izquierdo
Affiliation:
Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana-Xochimilco.
Roberto Martínez Rodríguez
Affiliation:
Centro de Enseñanza, investigación y extensión en producción porcina.
María Elena Trujillo Ortega
Affiliation:
Departamento de Producción Animal Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México D.F. C.P. 04510, México.
*
All correspondence to: Santiago René Anzaldúa Arce. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510, México, D.F. México, Tel:/Fax: +5255 5622 5893. e-mail: [email protected]
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Summary

We evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT.

Keywords

Boar spermatozoaGlycerolprotease inhibitorsPerinuclear theca substructure
Type
Research Article
Information
Zygote , Volume 21 , Issue 2 , May 2013 , pp. 172 - 177
Copyright
Copyright © Cambridge University Press 2012

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