Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-28T22:07:19.817Z Has data issue: false hasContentIssue false
  • English
  • Français

Follicular interactions affect the in vitro development of isolated goat preantral follicles

Published online by Cambridge University Press:  28 October 2010

Ana Beatriz Graça Duarte ,
Roberta Nogueira Chaves ,
Valdevane Rocha Araújo ,
Juliana Jales Celestino ,
Gerlane Modesto Silva ,
Cláudio Afonso Pinho Lopes ,
Líliam Mara Trevisan Tavares ,
Cláudio Cabral Campelo  and
José Ricardo de Figueiredo
Ana Beatriz Graça Duarte*
Affiliation:
Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Faculdade de Veterinária (FAVET), Universidade Estadual do Ceará (UECE), Av. Paranjana 1700, Campus do Itaperi, Fortaleza–CE, CEP: 60740–930, Brazil.
Roberta Nogueira Chaves
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Valdevane Rocha Araújo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Juliana Jales Celestino
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Gerlane Modesto Silva
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Cláudio Afonso Pinho Lopes
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Líliam Mara Trevisan Tavares
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
Cláudio Cabral Campelo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
José Ricardo de Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Veterinary Faculty, State University of Ceará, Fortaleza, Ceará, Brazil.
*
All correspondence to: Beatriz Duarte. Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Faculdade de Veterinária (FAVET), Universidade Estadual do Ceará (UECE), Av. Paranjana 1700, Campus do Itaperi, Fortaleza–CE, CEP: 60740–930, Brazil. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of this study was to evaluate the influence of the number of follicles per drop (one or three) and antral follicles on in vitro development of isolated goat preantral follicles. Preantral follicles were isolated through microdissection and distributed individually (control) or in groups of three follicles (treatment) in microdroplets of α-MEM with or without 1000 ng/ml follicle stimulating hormone (FSH) for Experiments 1 and 2, respectively. Experiment 3 was divided into four treatments according to the presence of one or three preantral follicles, associated or not with antral follicles. After culture, oocytes were retrieved from morphologically normal follicles and submitted to in vitro maturation (IVM) and live/dead fluorescent labelling. Results of Experiment 1 (basic medium without FSH) showed that culture of preantral follicles in groups enhances viability, growth and antrum formation after 12 days. However, in the presence of FSH (Experiment 2), only the recovery rate of fully grown oocytes for IVM was significantly affected by grouping of follicles. In Experiment 3, in general, co-culture of preantral follicles with an early antral follicle had a detrimental effect on viability, antrum formation and production of oocytes for IVM. In conclusion, the performance of in vitro culture of goat preantral follicles is affected by the number of follicles per drop, the presence of an antral follicle and FSH.

Keywords

CaprineGrowthMaturationOocyteViability
Type
Research Article
Information
Zygote , Volume 19 , Issue 3 , August 2011 , pp. 215 - 227
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adriaens, I., Cortvrindt, R. & Smitz, J. (2004). Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. Hum. Reprod. 19, 398–408.CrossRefGoogle ScholarPubMed
Albertini, D.F., Combelles, C.M., Benecchi, E. & Carabatsos, M.J. (2001). Cellular basis for paracrine regulation of ovarian follicle development. Reproduction 121, 647–53.CrossRefGoogle ScholarPubMed
Armstrong, D.G. & Webb, R. (1997). Ovarian follicular dominance: the role of intraovarian growth factors and novel proteins. Rev. Reprod. 2, 139–46.CrossRefGoogle ScholarPubMed
Baker, S.J., Srsen, V., Lapping, R. & Spears, N. (2001). Combined effect of follicle–follicle interactions and declining follicle-stimulating hormone on murine follicle health in vitro. Biol. Reprod. 65, 1304–10.CrossRefGoogle ScholarPubMed
Baker, S.J. & Spears, N. (1999). The role of intra-ovarian interactions in the regulation of follicle dominance. Hum. Reprod. 5, 155–65.Google ScholarPubMed
Cahill, L.P., Driancourt, M.A., Chamely, W.A & Findlay, J.K. (1985). Role of intrafollicular regulators and FSH in growth and development of large antral follicles in sheep. J. Reprod. Fertil. 75, 599–607.CrossRefGoogle ScholarPubMed
Cortvrindt, R., Smitz, J. & Van Steirteghem, A.C. (1997). Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. Hum. Reprod. 12, 759–68.CrossRefGoogle ScholarPubMed
Cortvrindt, R., Smitz, J. & Van Steirteghem, A.C. (1996). In vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum. Reprod. 11, 2656–66.CrossRefGoogle Scholar
Crozet, N., Ahmed-Ali, M. & Dubos, M.P. (1995). Developmental competence of goat oocytes from follicles of different size categories following maturation, fertilization and culture in vitro. J. Reprod. Fertil. 103, 293–98.CrossRefGoogle ScholarPubMed
Crozet, N., Dahirel, M. & Gall, L. (2000). Meiotic competence of in vitro grown goat oocytes J. Reprod. Fertil. 118, 367–73.CrossRefGoogle ScholarPubMed
De Smedt, V., Crozet, N. & Gall, L. (1994). Morphological and functional changes accompanying the acquisition of meiotic competence in ovarian goat oocyte. J. Exp. Zool. 269, 128–39.CrossRefGoogle ScholarPubMed
Demeestere, I., Centner, J., Gervy, C., Englert, Y. & Delbaere, A. (2005). Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents. Reproduction. 130, 147–57.CrossRefGoogle ScholarPubMed
Demeestere, I., Simon, P.Englert, Y. & Delbaere, A. (2003). Preliminary experience of ovarian tissue cryopreservation procedure: alternatives, perspectives and feasibility. Reprod. Biomed. Online 7, 572–79.CrossRefGoogle ScholarPubMed
Eppig, J.J. (1991). Maintenance of meiotic arrest and the induction of oocyte maturation in mouse oocyte–granulosa cell complexes developed in vitro from preantral follicles. Biol. Reprod. 45, 824–30.CrossRefGoogle ScholarPubMed
Eppig, J.J. & O'Brien, M.J. (1996). Development in vitro of mouse oocytes from primordial follicles. Biol. Reprod. 54, 197–207.CrossRefGoogle ScholarPubMed
Fukui, Y., Lee, E.S. & Araki, N. (1996). Effect of medium renewal during culture in two different culture systems on development to blastocysts from in vitro produced early bovine embryos. J. Anim. Sci. 74, 2752–58.CrossRefGoogle ScholarPubMed
Gao, M., Wang, Y. & Wu, X. (2007). In vitro maturation of immature oocytes from preantral follicles in prepuberal mice. J. Reprod. Contracept. 18, 25–32.CrossRefGoogle Scholar
Gardner, D.K, Lane, M., Spitzer, A. & Batt, P.A. (1994). Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development. Biol. Reprod. 50, 390–400.CrossRefGoogle Scholar
Gardner, D.K. & Lane, M. (1993). Amino acids and ammonium regulate mouse embryo development in culture. Biol. Reprod. 48, 377–85.CrossRefGoogle ScholarPubMed
Gupta, P.S.P, Ramesh, H.S., Manjunatha, B.M., Nandi, S. & Ravindra, J.P. (2008). Production of buffalo embryos using oocytes from in vitro grown preantral follicles. Zygote 16, 57–63.CrossRefGoogle ScholarPubMed
Gupta, P.S.P., Nandi, S., Ravindranatha, B.M. & Sarma, P.V. (2002). In vitro culture of buffalo (Bubalus bubalis) preantral follicles. Theriogenology 57, 1839–54.CrossRefGoogle ScholarPubMed
Gutierrez, C.G., Ralph, J.H., Telfer, E.E., Wilmut, I. & Webb, R. (2000). Growth and antrum formation of bovine preantral follicles in long-term culture in vitro. Biol. Reprod. 62, 1322–28.CrossRefGoogle ScholarPubMed
Haidari, K., Salehnia, M. & Valojerdi, M.R. (2008). The effect of leukemia inhibitory factor and co-culture on the in vitro maturation and ultrastructure of vitrified isolated mouse preantral follicles. Fertil. Steril. 90, 2389–97.CrossRefGoogle ScholarPubMed
Han, Z., Lan, G., Wu, Y., Han, D., Feng, W., Wang, J. & Tan, J. (2006). Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system. Reproduction 132, 749–58.CrossRefGoogle ScholarPubMed
Kreeger, P.K., Fernandes, N.N., Woodruff, T.K. & Shea, L.D. (2005). Regulation of mouse follicle development by follicle-stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose. Biol. Reprod. 73, 942–50.CrossRefGoogle Scholar
Wu, M.F., Huang, W.T., Tsay, C., Hsu, H.F., Liu, B.T., Chiou, C.M., Yen, S.C., Cheng, S.P. & Ju, J.C. (2002). The stage-dependent inhibitory effect of porcine follicular cells on the development of preantral follicles Anim. Reprod. Sci. 73, 73–88.CrossRefGoogle ScholarPubMed
Mao, J., Smith, M.F., Rucker, E.B., Wu, G.M., Mccauley, T.C., Cantley, T.C., Prather, R.S., Didion, B.A. & Day, B.N. (2004). Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro. J. Anim. Sci. 82, 1967–75.CrossRefGoogle ScholarPubMed
Mao, J., Wu, G., Smith, M.F., Mccauley, T.C, Cantley, T.C., Prather, R.S., Didion, B.A. & Day, B.N. (2002). Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67, 1197–1203.CrossRefGoogle ScholarPubMed
Metoki, T., Iwata, H., Itoh, M., Kasai, M., Takajyo, A., Suzuki, A., Kuwayama, T. & Monji, Y. (2008). Effects of follicular fluids on the growth of porcine preantral follicle and oocyte. Zygote 16, 239–47.CrossRefGoogle ScholarPubMed
Mitchell, L.M., Kennedy, C.R. & Hartshorne, G.M. (2002). Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro. Hum. Reprod. 17, 1181–88.CrossRefGoogle ScholarPubMed
Nayudu, P.L. & Osborn, S.M. (1992). Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fert. 95, 349–62.CrossRefGoogle ScholarPubMed
O'Brien, M.J., Pendola, J.K. & Eppig, J.J. (2003). A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their development competence. Biol. Reprod. 8, 1682–86.CrossRefGoogle Scholar
Spears, N., De Bruin, J.P. & Gosden, R.G. (1996). The establishment of follicular dominance in co-cultured mouse ovarian follicles. J. Reprod. Fertil. 106, 1–6.CrossRefGoogle ScholarPubMed
Spears, N., Baker, S., Srsen, V., Lapping, R., Mullan, J., Nelson, R. & Allison, V. (2002). Mouse ovarian follicles secrete factors affecting the growth and development of like-sized ovarian follicles in vitro. Biol. Reprod. 67, 1726–33.CrossRefGoogle ScholarPubMed
Telfer, E.E. (1998). In vitro models for oocyte development. Theriogenology 49, 451–60.CrossRefGoogle ScholarPubMed
Trounson, A.O., Pushett, D., Maclellan, L.J., Lewis, I. & Gardner, D.K. (1994). Current status of IVM/IVF and embryo culture in humans and farm animals. Theriogenology 4, 57–66.CrossRefGoogle Scholar
Wandji, S.A., Srsen, V., Nathanielsz, P.W., Eppig, J.J. & Fortune, J.E. (1997). Initiation of growth of baboon primordial follicles in vitro. Hum. Reprod. 12, 1993–2001.CrossRefGoogle ScholarPubMed
Wang, Y., Rippstein, P.U. & Tsang, B.K. (2003). Role and gonadotrophic regulation of X-linked inhibitor of apoptosis protein expression during rat ovarian follicular development in vitro. Biol. Reprod. 68, 610–19.CrossRefGoogle ScholarPubMed
Wu, J., Emery, B.R. & Carrell, D.T. (2001). In vitro growth, maturation, fertilization, and embryonic development of oocytes from porcine preantral follicles. Biol. Reprod. 64, 375–81.CrossRefGoogle ScholarPubMed
Zhou, H.M. & Zhang, Y. (2006). In vitro growth and maturation of caprine oocytes. Reprod. Dom. Anim. 41, 444–47.CrossRefGoogle ScholarPubMed