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First polar body morphology affects potential development of porcine parthenogenetic embryo in vitro

Published online by Cambridge University Press:  03 July 2014

Junhe Hu ,
Chenzhong Jin ,
Hui Zheng ,
Qinyan Liu ,
Wenbing Zhu ,
Zhi Zeng ,
Juan Wu ,
Yang Wang ,
Jie Li  and
Xuejiao Zhang
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Junhe Hu*
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan Province, 417000, China
Chenzhong Jin
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Hui Zheng
Affiliation:
Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China.
Qinyan Liu
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Wenbing Zhu
Affiliation:
Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China.
Zhi Zeng
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Juan Wu
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Yang Wang
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Jie Li
Affiliation:
Xiangya Medical School, Central South University, Changsha, Hunan 410078, P.R. China.
Xuejiao Zhang
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Xianglin Liu
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
Jian Zhao
Affiliation:
Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan 417000, P.R. China.
*
All correspondence to: Junhe Hu. Department of Life Sciences, Hunan University of Humanities, Science and Technology (HUHST), Loudi City, Hunan Province, 417000, China. Tel:+ 86 0738 8372053. Fax: +86 0738 8372053. e-mail address: [email protected]
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Summary

Previous studies have reported that the first polar body (PB1) morphology reflects embryo development competence, but the effects of PB1 on porcine embryo development remain unknown. This study aims to determine whether the ability of porcine embryo development is related to oocytes’ PB1 in vitro. The distribution of type II cortical granules (CGs) of porcine matured oocytes in grade B PB1 is significantly greater compared with those in grades A and C PB1 (71.43% versus 52.46% and 50%; P < 0.05). The ratio of porcine parthenogenetic blastocysts and the mean cell number in each blastocyst in the group with grade B PB1 is significantly greater than that with grades A and C PB1 (30.81% vs. 19.02% and 15.15%; P < 0.05) and (36.67 versus 24.67, 28.67; P < 0.05), and no significant differences are found in the embryo cleavage for all groups (79.75%, 84.30%, and 78.18% in grades A, B, and C PB1; P > 0.05). The acetylation level of porcine embryos in the group with grade B PB1 is significantly greater compared with those in the other groups (P < 0.05), and is almost 2.5 times higher than that in grade A. Therefore, porcine oocytes with PB1 in grade B are more competitive in cytoplasmic maturation and further embryo development in vitro.

Keywords

AcetylationFirst polar body (PB1)OocytePorcine
Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 615 - 621
Copyright
Copyright © Cambridge University Press 2014 

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References

Albertini, D.F., Sanfins, A. & Combelles, C.M.H. (2003). Origins and manifestations of oocyte maturation competencies. Reprod. BioMed. Online 6, 410–15.CrossRefGoogle ScholarPubMed
Antosik, P., Kempisty, B., Jackowska, M., Bukowska, D., Lianeri, M., Brussow, K., Wozna, M. & Jaskowski, J. (2010). The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2 protein levels. Vet. Med. 55, 154–62.CrossRefGoogle Scholar
Coticchio, G., Sereni, E., Serrao, L., Mazzone, S., Iadarola, I. & Borini, A. (2004). What criteria for the definition of oocyte quality? Ann. N. Y. Acad. Sci. 1034, 132–44.CrossRefGoogle ScholarPubMed
Das, Z.C., Gupta, M.K., Uhm, S.J. & Lee, H.T. (2010). Increasing histone acetylation of cloned embryos, but not donor cells, by sodium butyrate improves their in vitro development in pigs. Cell. Reprogram. 12, 95–104.CrossRefGoogle Scholar
De Santis, L., Cino, I., Rabellotti, E., Calzi, F., Persico, P., Borini, A. & Coticchio, G. (2005). Polar body morphology and spindle imaging as predictors of oocyte quality. Reprod. BioMed. Online 11, 36–42.CrossRefGoogle ScholarPubMed
De Vos, A., Van de Velde, H., Joris, H. & Van Steirteghem, A. (1999). In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum. Reprod. 14, 1859–63.CrossRefGoogle ScholarPubMed
Funahashi, H., Koike, T. & Sakai, R. (2008). Effect of glucose and pyruvate on nuclear and cytoplasmic maturation of porcine oocytes in a chemically defined medium. Theriogenology 70, 1041–7.CrossRefGoogle Scholar
Gupta, M.K., Uhm, S.J. & Lee, H.T. (2008). Sexual maturity and reproductive phase of oocyte donor influence the developmental ability and apoptosis of cloned and parthenogenetic porcine embryos. Anim. Reprod. Sci. 108, 107–21.CrossRefGoogle ScholarPubMed
Hosoe, M. & Shioya, Y. (1997). Distribution of cortical granules in bovine oocytes classified by cumulus complex. Zygote 5, 371–6.CrossRefGoogle ScholarPubMed
Hu, J., Ma, X., Bao, J.C., Li, W., Cheng, D., Gao, Z., Lei, A., Yang, C. & Wang, H. (2011). Insulin–transferrin–selenium (ITS) improves maturation of porcine oocytes in vitro. Zygote 19, 191–7.CrossRefGoogle ScholarPubMed
Navarro, P.A., de Araújo, M.M., de Araújo, C.M., Rocha, M., dos Reis, R. & Martins, W. (2009). Relationship between first polar body morphology before intracytoplasmic sperm injection and fertilization rate, cleavage rate, and embryo quality. Int. J. Gynecol. Obstet. 104, 226–9.CrossRefGoogle ScholarPubMed
Rose, B. & Laky, D. (2013). Polar body fragmentation in IVM oocytes is associated with impaired fertilization and embryo development. J. Assist. Reprod. Genet. 30, 679–82.CrossRefGoogle ScholarPubMed
Santos, F., Peters, A.H., Otte, A.P., Reik, W. & Dean, W. (2005). Dynamic chromatin modifications characterise the first cell cycle in mouse embryos. Dev. Biol. 280, 225–36.CrossRefGoogle ScholarPubMed
Scott, L., Finn, A., O’Leary, T., McLellan, S. & Hill, J. (2007). Morphologic parameters of early cleavage-stage embryos that correlate with fetal development and delivery: prospective and applied data for increased pregnancy rates. Hum. Reprod. 22, 230–40.CrossRefGoogle ScholarPubMed
Segers, I., Adriaenssens, T., Coucke, W., Cortvrindt, R. & Smitz, J. (2008). Timing of nuclear maturation and postovulatory aging in oocytes of in vitro-grown mouse follicles with or without oil overlay. Biol. Reprod. 78, 859–68.CrossRefGoogle ScholarPubMed
Ten, J., Mendiola, J., Vioque, J., de Juan, J. & Bernabeu, R. (2007). Donor oocyte dysmorphisms and their influence on fertilization and embryo quality. Reprod. BioMed. Online 14, 40–8.CrossRefGoogle ScholarPubMed
Van de Velde, H., Nagy, Z.P., Joris, H., De Vos, A. & Van Steirteghem, A.C. (1997). Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection. Hum. Reprod. 12, 2246–50.CrossRefGoogle ScholarPubMed
Vanhoutte, L., De Sutter, P., Nogueira, D., Gerris, J., Dhont, M. & Van der Elst, J. (2007). Nuclear and cytoplasmic maturation of in vitro matured human oocytes after temporary nuclear arrest by phosphodiesterase 3-inhibitor. Hum. Reprod. 22, 1239.CrossRefGoogle ScholarPubMed
Wang, W.-H., Sun, Q.-Y., Hosoe, M., Shioya, Y. & Day, B.N. (1997). Quantified analysis of cortical granule distribution and exocytosis of porcine oocytes during meiotic maturation and activation. Biol. Reprod. 56, 1376–82.CrossRefGoogle ScholarPubMed
Wang, F., Kou, Z., Zhang, Y. & Gao, S. (2007). Dynamic reprogramming of histone acetylation and methylation in the first cell cycle of cloned mouse embryos. Biol. Reprod. 77, 1007–16.CrossRefGoogle ScholarPubMed
Yamanaka, K., Sugimura, S., Wakai, T., Kawahara, M. & Sato, E. (2009). Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos. J. Reprod. Dev. 55, 638–44.CrossRefGoogle ScholarPubMed
Younis, J.S., Radin, O., Izhaki, I. & Ben-Ami, M. (2009). Does first polar body morphology predict oocyte performance during ICSI treatment? J. Assist. Reprod. Genet. 26, 561–7.CrossRefGoogle ScholarPubMed