Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-16T15:28:58.295Z Has data issue: false hasContentIssue false
  • English
  • Français

Fertilization, hatching, and embryogenesis of diploid and triploid eggs of Anabas testudineus (Bloch, 1792)

Published online by Cambridge University Press:  09 October 2018

A. Hassan ,
V.T. Okomoda Open the ORCID record for V.T. Okomoda [Opens in a new window]  and
F.A.B. Sanusi
A. Hassan*
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria
V.T. Okomoda
Affiliation:
School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
F.A.B. Sanusi
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria
*
All correspondence to: A. Hassan. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria. Tel: +60199360392. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

This study investigated the breeding parameters and embryogenic development of diploid and heat shock-induced triploid eggs of Anabas testudineus (Bloch, 1792). To this effect, broodstocks of A. testudineus were induced to spawn using the Ovaprim® hormone. After fertilization, the eggs were divided into two groups and one portion heat shocked at 41°C (for 3 min), at approximately 4 min after fertilization. Results of fertilization, hatchability, as well as the sequence and timing of embryogenic development were collated from three breeding trials. Fertilization percentages were similar in both treatments (≈90%) while hatchability was higher in the diploid eggs (79.56%) than the triploid induced eggs (50.04%). Both treatments had the same sequence of embryogenetic stages; however, the timing of development was significantly delayed in the triploids (i.e. beyond the 2-cell stages) as compared with the observations in the control group (diploid eggs). Consequently, hatching time was 5 h faster in the diploid eggs [i.e. 18 hours post fertilization (hpf)] compared with the triploid induced eggs (23 hpf). The most critical stage of embryonic development in which mass mortality occurred in the different treatments was the somite stage. The status of triploid hatchlings was affirmed using erythrocyte morphology in 2-month-old fingerlings.

Keywords

Climbing perchEmbryogenesisErythrocyte morphologyHeat shockSpawning performance
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 343 - 349
Copyright
© Cambridge University Press, 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allen, S.K. Jr & Stanley, J.G. (1978). Reproductive sterility in polyploid brook trout. Salvelinus fontinalis . Trans. Am. Fish. Soc. 107, 473–478.Google Scholar
Aluko, P.O. (1995). Growth characteristics of first, second and backcross generations of the hybrids between Heterobranchus longifilis and Clarias anguillaris. In 1995 Annual Report. National Institute for Freshwater Fisheries Research, New Bussa, Nigeria, pp. 74–8.Google Scholar
Arockiaraj, A.J., Haniffa, M.A., Seetharaman, S. & Sing, S.P. (2003). Early development of a threatened freshwater catfish Mystus montanus (lerdon): perspective on teleost gastrulation. Am. Natur. 144, 133–152.Google Scholar
Aydın, I. & Okumus, I. (2017). Effects of triploid induction to the early cleavage patterns of black sea turbot (Psetta maxima) embryos. Genetics of Aquatic Organisms 1, 15–20.Google Scholar
Benfey, T.J. (1999). The physiology and behavior of triploid fishes. Rev. Fish. Sci. 7, 39–67.Google Scholar
Berrill, I.K., MacIntyre, C.M., Noble, C., Kankainen, M. & Turnbull, J.F. (2012). Bio-economic costs and benefits of using triploid rainbow trout in aquaculture: Reduced mortality. Aquacult. Econ. Manage. 16, 365–383.Google Scholar
Brown, L.A. & Roberts, R.J. (1982). Production of neutered salmonids. Comp. Biochem. Physiol. 73, 177–180.Google Scholar
Buzollo, H., Verıssimo-Silveira, R., Oliveira-Almeida, I.R., Alexandre, J.S., Okuda, H.T. & Ninhaus-Silveira, A. (2011). Structural analysis of the Pimelodus maculatus (Lacepele, 1803) embryogenesis (Siluriformes: Pimelodidae). Neotrop. Ichthyol. 9, 601–616.Google Scholar
Carman, O., Oshiro, T. & Takashima, F. (1991). Estimation of effective condition for induction of triploidy in goldfish Carassius auratus Linnaeus. J. Tokyo Univ. Fish 78, 127–135.Google Scholar
Chaturvedi, C.S., Ambulkar, R., Singh, R.K. & Pandey, A.K. (2015). Successful induced spawning of climbing perch, Anabas testudineus (Bloch, 1792), under controlled conditions at Raipur (Chhattisgarh), India. Indian Natl J. Life Sci. 12, 209–214.Google Scholar
Chondar, S.L. (1999). Biology of Finfish and Shellfish, SCSC Pub. (India): Howrah.Google Scholar
Cotter, D., O’Donovan, V., O’Maoileidigh, N., Rogan, G., Roche, N. & Wilkins, N.P. (2000). An evaluation of the use of triploid Atlantic salmon (Salmo salar L.) in minimising the impact of escaped farmed salmon on wild populations. Aquaculture 186, 61–75.Google Scholar
da Rocha, P.V., Sato, Y., Rizzo, E. & Bazzoli, N. (2009). Biology of eggs, embryos and larvae of Rhinelepis aspera (Spix & Agassiz, 1829) (Pisces: Siluriformes). Zygote 18, 159–171.Google Scholar
Devauchelle, N., Alexandre, J.C., Le Corre, N. & Letty, Y. (1988). Spawning of turbot (Scophthalmus maximus), in captivity. Aquaculture 69, 159–184.Google Scholar
Dorafshan, S.M., Kalbassi, M.R., Pourkazemi, M., Amiri, M.B. & Karimi, S.S. (2008). Effect of triploidy on the Caspian salmon, Salmo trutta caspius haematology. Fish Physiol. Biochem. 34, 195–200.Google Scholar
Dunham, R.A. (2004). Aquaculture and Fisheries Biotechnology: Genetic Approaches . Cambridge, Mass: CABI Publishing, 372 pp.Google Scholar
Felip, A., Zanuy, S., Carrillo, M. & Piferrer, F. (2001). Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species. Genetica 111, 175–195.Google Scholar
Fraser, T.W.K., Fjelldal, P.R., Hansen, T. & Mayer, I. (2012). Welfare considerations of triploid fish. Rev. Fish. Sci. 20, 192–211.Google Scholar
Gao, Z., Wang, W., Abbas, K., Zhou, X., Yang, Y., Diana, J.S., Wang, H., Li, Y. & Sun, Y. (2007). Haematological characterization of loach Misgurnus anguillicaudatus: comparison among diploid, triploid and tetraploid specimens. Comp. Biochem. Physiol. A. 147, 1001–1008.Google Scholar
Happe, A., Quillet, E. & Chevassus, B. (1988). Early life history of triploid rainbow trout (Salmo gairdneri Richardson). Aquaculture 71 (1–2):107–118.Google Scholar
Honji, R.M., Tolussi, C.E., Mello, P. H., Caneppele, D. & Moreira, R. G. (2012). Embryonic development and larval stages of Steindachneridion parahybae (Siluriformes: Pimelodidae) – implications for the conservation and rearing of this endangered neotropical species. Neotrop. Ichthyol. 10, 313–327.Google Scholar
Jayaram, K.C. (2010). The Freshwater Fishes of the Indian Region, 2nd edn, Narendra Pub. House: Delhi.Google Scholar
Karami, A., Christianus, A., Ishak, Z., Courtenay, S.C., Syed, M.A., Noor Azlina, M. & Noorshinah, H. (2010). Effect of triploidization on juvenile African catfish. Aquacult. Int. 18, 851–858.Google Scholar
Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B. & Schilling, T.F. (1995). Stages of embryonic development of the zebrafish. Dev. Dyn. 203, 253–310.Google Scholar
Kjørsvik, E.A., Mangorjensen, A. & Holmefjord, I. (1990). Egg quality in fishes. Adv Mar. Biol. 26, 71–113.Google Scholar
Kjørsvik, E., Hoehne-Reitan, K. & Reitan, K.I. (2003). Egg and larval quality criteria as predictive measure for juvenile production in turbot (Scophthalmus maximus L.). Aquaculture 227, 9–20.Google Scholar
Koedprang, W. & Na-Nakorn, U. (2000). Preliminary study on performance of triploid Thai silver barb. Puntius gonionotus. Aquaculture 190, 211–221.Google Scholar
Legatt, R.A. & Iwama, G.K. (2003). Occurrence of polyploidy in the fifishes. Rev. Fish Biol. Fish. 13, 237–246.Google Scholar
Lincoln, R.F. & Scott, A.P. (1983). Production of all-female triploid rainbow trout. Aquaculture 30, 375–380.Google Scholar
Makino, S. & Ozima, Y. (1943). Formation of the diploid egg nucleus due to suppression of the second maturation division, induced by refrigeration of fertilized eggs of the carp. Cyprinus carpio. Cytologia 13, 55–60.Google Scholar
Maxime, V. (2008). The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish Fish. 9, 67–78.Google Scholar
Neal, J.W. (2003). Live fast and die young: on the growth and mortality of largemouth bass in Puerto Rico. Ph.D. thesis, North Carolina State University, Raleigh, 137 pp.Google Scholar
Nell, J.A. (2002). Farming triploid oysters. Aquaculture 210, 69–88.Google Scholar
Ninhaus-Silveira, A., Foresti, F. & Azevedo, A. (2006). Structural and ultrastructural analysis of embryonic development of Prochilodus lineatus (Valenciennes, 1836) (Characiformes, Prochilodontinae). Zygote 14, 217–229.Google Scholar
Normala, J., Mohd, A.A., Abol, M.A.B., Nur, A.A., Khor, W., Okomoda, V.T. & Shahreza, M.S. (2017). Morphometric variations between triploid and diploid Clarias gariepinus (Burchell, 1822). Croatian J. Fish. 75, 113–121.Google Scholar
Normala, J., Mohd, A.A., Abol, M.A.B., Nur, A.A., Khor, W. & Shahreza, M.S. (2016). It is all in the blood: erythrocyte characterization of triploid and diploid african catfish, Clarias gariepinus . J. Fish. Aquat. Sci. 11, 425–431.Google Scholar
Okomoda, V.T., Koh, I.C.C., Hassan, A., Amornsakun, T. & Shahreza, M.S. (2017). Embryonic and larvae development of reciprocal crosses between Pangasianodon hypophthalmus (Sauvage, 1878) and Clariasgariepinus (Burchell, 1822). Egypt. J. Aquat. Res. 43, 321–327.Google Scholar
Okomoda, V.T., Koh, I.C.C. & Shahreza, M.S. (2018). A simple technique for accurate estimation of fertilization rate with specific application to Clarias gariepinus (Burchell, 1822). Aquac. Res. 49, 1116–1121.Google Scholar
Olaniyi, W.A. & Omitogun, O.G. (2013). Stages in the early and larval development of the African catfish Clarias gariepinus (Teleostei, Clariidae). Zygote 22, 314–330.Google Scholar
Olufeagba, S.O., Aluko, P.O., Omotosho, J.S., Oyewole, S.O. & Raji, A. (1999). Triploid induction in Heterobranchus longifilis by cold shock. Proceedings of the 13th annual conference of FISON, New-Bussa (ed. A.A. Eyo), pp. 247–251.Google Scholar
Olufeagba, S.O., Okomoda, V.T. & Shaibu, G. (2016). Embryogenesis and early growth of pure strains and hybrids between Clarias gariepinus and Heterobranchus longifilis . North Am. J. Aquac. 78, 346–355.Google Scholar
Pal, M. & Chaudhry, S. (2010). A. testudineus. In The IUCN Red List of Threatened species. http://dx.doi.org/10.22305/IUCN.UK. 2010–4.RLTS. T1665434A6232945.en.Google Scholar
Pickova, J., Dutta, P.C., Larsson, P.O. & Kiessling, A. (1997). Early embryonic cleavage pattern, hatching success & egg-lipid fatty acid composition: comparison between two cod (Gadus morhua) stocks. Can. J. Fish. Aquat. Sci. 54, 2410–2416.Google Scholar
Piferrer, F., Beaumont, A., Falguiere, J-C, Flajshans, M., Haffray, P. & Colombo, L. (2009). Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293, 125–156.Google Scholar
Piferrer, F., Cal, R.M., Álvarez-Blázquez, B., Sánchez, L. & Martinez, P. (2000). Induction of triploidy in the turbot (Scophthalmus maximus). I. Ploidy determination and the effects of cold shocks. Aquaculture 188, 79–90.Google Scholar
Piferrer, F., Cal, R.M., Gómez, C., Bouza, B. & Martinez, P. (2003). Induction of triploidy in the turbot (Scophthalmus maximus). II. Effects of cold shock timing and induction of triploidy in a large volume of eggs. Aquaculture 220, 821–831.Google Scholar
Pradeep, P.J., Srijaya, T.C., Jose, D., Papini, A., Hassan, A. & Chatterji, A.K. (2011). Identification of diploid and triploid red tilapia using erythrocyte indices. Caryologia 64, 485–492.Google Scholar
Svardson, G. (1945). Chromosome studies on Salmonidae. Reports from the Swedish State Institute of Freshwater Fishery. Research, Drottningholm 23, 1–151.Google Scholar
Talwar, P.K. & Jhingran, A.G. (1991). Inland Fishes of India and Adjacent Countries. vols 1 and 2 Oxford & IBH Pub. Co., New Delhi, p. 1158.Google Scholar
Thorgaard, G.H. & Gall, G.A. (1979). Adult triploids in a rainbow trout family. Genetics 93, 961–973.Google Scholar
Thorgaard, G.H. (1983). Chromosome set manipulation and sex control in fish. Fish Physiol. 9 (B):405–434.Google Scholar
Tiwary, B.K., Kirubagaran, R. & Ray, A.K. (2004). The biology of triploid fish. Rev. Fish Biol. Fish. 14, 391–402.Google Scholar
Varadaraj, K. & Pandian, T.J. (1990). Production of all female sterile triploid Oreochromis mossambicus . Aquaculture 84, 117–123.Google Scholar