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Expression of XNOA 36 in the mitochondrial cloud of Xenopus laevis oocytes

Published online by Cambridge University Press:  15 April 2011

M.C. Vaccaro ,
M. Wilding ,
B. Dale ,
C. Campanella  and
R. Carotenuto
M.C. Vaccaro
Affiliation:
Department of Structural and Functional Biology, University of Naples ‘Federico II’, Italy.
M. Wilding
Affiliation:
Centre for Assisted Fertilization, Clinica Villa del Sole, Naples, Italy.
B. Dale
Affiliation:
Centre for Assisted Fertilization, Clinica Villa del Sole, Naples, Italy.
C. Campanella
Affiliation:
Department of Structural and Functional Biology, University of Naples ‘Federico II’, Italy.
R. Carotenuto*
Affiliation:
Department of Structural and Functional Biology. University of Naples Federico II. Complesso Universitario Monte Sant'Angelo, Via Cinthia, I-80126, Napoli, Italy.
*
All correspondence to: Rosa Carotenuto, Department of Structural and Functional Biology. University of Naples Federico II. Complesso Universitario Monte Sant'Angelo, Via Cinthia, I-80126, Napoli, Italy. Tel: +39 081 679195. Fax: +39 081 679233. e-mail: [email protected]
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Summary

In Xenopus laevis oocytes a mitochondrial cloud (MC) is found between the nucleus and the plasma membrane at stages I–II of oogenesis. The MC contains RNAs that are transported to the future vegetal pole at stage II of oogenesis. In particular, germinal plasm mRNAs are found in the Message Transport Organiser (METRO) region, the MC region opposite to the nucleus. At stages II–III, a second pathway transports Vg1 and VegT mRNAs to the area where the MC content merges with the vegetal cortex. Microtubules become polarized at the sites of migration of Vg1 and VegT mRNAs through an unknown signalling mechanism. In early meiotic stages, the centrioles are almost completely lost with their remnants being dispersed into the cytoplasm and the MC, which may contain a MTOC to be used in the later localization pathway of the mRNAs. In mammals, XNOA 36 encodes a member of a highly conserved protein family and localises to the nucleolus or in the centromeres. In the Xenopus late stage I oocyte, XNOA 36 mRNA is transiently segregated in one half of the oocyte, anchored by a cytoskeletal network that contains spectrin. Here we found that XNOA 36 transcript also localises to the nucleoli and in the METRO region. XNOA 36 protein immunolocalization, using an antibody employed for the library immunoscreening that depicted XNOA 36 expression colonies, labels the migrating MC, the cytoplasm of stage I oocytes and in particular the vegetal cortex facing the MC. The possible role of XNOA 36 in mRNA anchoring to the vegetal cortex or in participating in early microtubule reorganization is discussed.

Keywords

CytoskeletonMicrotubulesMitochondrial cloudOocyteXenopus laevis
Type
Research Article
Information
Zygote , Volume 20 , Issue 3 , August 2012 , pp. 237 - 242
Copyright
Copyright © Cambridge University Press 2011

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