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Expression levels of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells can predict in vitro developmental competence of bovine oocytes

Published online by Cambridge University Press:  02 June 2020

Atchalalt Khurchabilig Open the ORCID record for Atchalalt Khurchabilig [Opens in a new window] ,
Akane Sato ,
Shiori Ashibe ,
Asuka Hara ,
Rika Fukumori  and
Yoshikazu Nagao
Atchalalt Khurchabilig
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo183–8509, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
Akane Sato
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
Shiori Ashibe
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo183–8509, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
Asuka Hara
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
Rika Fukumori
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
Yoshikazu Nagao*
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo183–8509, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi321–4415, Japan
*
Author for correspondence: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan. Tel:/Fax: +81 285 84 2426. E-mail: [email protected]
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Summary

The efficiency of in vitro embryo production technologies would be improved by the development of suitable non-invasive biomarkers that allow the selection of good quality cumulus–oocyte complexes (COCs). The present study used whole, single oocyte culture to investigate whether the expression levels of follicle-stimulating hormone receptor (FSHR), insulin-like factor 1 receptor (IGF1R) and three steroidogenesis-related enzymes (CYP11al, CYP19al and HSD3β) in cumulus cells reflected the developmental competence of COCs. Cumulus cells were collected from single COCs before maturation culture and relative mRNA levels were assessed using real-time PCR. The analysis indicated that mRNAs for FSHR, IGF1R, CYP11al and HSD3β were present at higher levels in cumulus cells from COCs that failed to form blastocysts compared with cumulus cells from COCs that formed blastocysts. Moreover, FSHR and IGF1R mRNA levels were positively correlated with those of genes for steroidogenesis-related enzymes. In conclusion, poor developmental competence of COCs was related to higher expression of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells, which may indicate the advanced differentiation of cumulus cells into granulosa cells.

Keywords

BiomarkersBovine oocyteCumulus cellDevelopmental competencemRNA
Type
Research Article
Information
Zygote , Volume 28 , Issue 5 , October 2020 , pp. 425 - 431
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Present address: Department of Health and Environmental Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

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