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Environment and medium volume influence in vitro fertilisation of pig oocytes

Published online by Cambridge University Press:  26 September 2008

P. Coy ,
E. Martínez ,
S. Ruiz ,
J.M. Vázquez ,
J. Roca  and
J. Gadea
P. Coy*
Affiliation:
University of Murcia, Murcia, Spain
E. Martínez
Affiliation:
University of Murcia, Murcia, Spain
S. Ruiz
Affiliation:
University of Murcia, Murcia, Spain
J.M. Vázquez
Affiliation:
University of Murcia, Murcia, Spain
J. Roca
Affiliation:
University of Murcia, Murcia, Spain
J. Gadea
Affiliation:
University of Murcia, Murcia, Spain
*
P. Coy, Departmento de Biologia Animal (Fisiología Animal), Facultad de Veterinaria, Campus de Espinardo, Universidad de Murcia, Aptdo. 4021, Murcia E-30071, Spain. Fax: 34-68 305101
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Summary

Oviductal oocytes (n = 795) were obtained from ovulation-induced prepubertal gilts. In the first experiment, different parameters related to in vitro fertilisation (IVF) were compared in the presence and absence of cumulus matrix (which is shed with the oocytes at ovulation.) The results show that the presence of this matrix is beneficial because the rates of fertilisation (69%) (number of monospermic oocytes/total number of healthy mature oocytes; 42%), and the median number of spermatozoa per oocyte (1.52±0.06), were improved with respect to those obtained in its absence (54%, 22% and 2.33 ± 0.08, respectively; p<0.01). In the second experiment the effect of two different volumes of co-incubation medium (2 ml and 0.4 ml) on the same parameters of porcine IVF were compared. No significant differences between volumes were observed, except in the mean number of spermtozoa per oocyte and the percentage of dispermic oocytes.

Keywords

CumulusIn vitro fertilisationMediumvolumeporine
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 209 - 213
Copyright
Copyright © Cambridge University Press 1993

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