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Effects of ghrelin on activation of Akt1 and ERK1/2 pathways during in vitro maturation of bovine oocytes

Published online by Cambridge University Press:  07 March 2017

Thomas-Markos Chouzouris ,
Eleni Dovolou ,
Fotini Krania ,
Ioannis S. Pappas ,
Konstantinos Dafopoulos ,
Ioannis E. Messinis ,
George Anifandis  and
Georgios S. Amiridis
Thomas-Markos Chouzouris
Affiliation:
Department of Obstetrics and Reproduction, Veterinary Faculty, School of Health Sciences, Karditsa, University of Thessaly, Greece.
Eleni Dovolou
Affiliation:
Department of Obstetrics and Reproduction, Veterinary Faculty, School of Health Sciences, Karditsa, University of Thessaly, Greece.
Fotini Krania
Affiliation:
Department of Obstetrics and Reproduction, Veterinary Faculty, School of Health Sciences, Karditsa, University of Thessaly, Greece.
Ioannis S. Pappas
Affiliation:
Department of Pharmacology, Veterinary Faculty, School of Health Sciences, Karditsa, University of Thessaly, Greece.
Konstantinos Dafopoulos
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Medicine, School of Health Sciences, Larissa, University of Thessaly, Greece.
Ioannis E. Messinis
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Medicine, School of Health Sciences, Larissa, University of Thessaly, Greece.
George Anifandis
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Medicine, School of Health Sciences, Larissa, University of Thessaly, Greece.
Georgios S. Amiridis*
Affiliation:
PO Box 199, 43100 Karditsa, Greece. Department of Obstetrics and Reproduction, Veterinary Faculty, School of Health Sciences, Karditsa, University of Thessaly, Greece.
*
All correspondence to: Georgios S. Amiridis. PO Box 199, 43100 Karditsa, Greece. Tel: +302441066073. E-mail [email protected]
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Summary

The purpose of this study was to investigate the possible molecular pathways through which ghrelin accelerates in vitro oocyte maturation. Bovine cumulus–oocyte complexes (COCs), after 18 or 24 h maturation in the absence or the presence of 800 pg ml–1 of acylated ghrelin were either assessed for nuclear maturation or underwent in vitro fertilization in standard media and putative zygotes were cultured in vitro for 8 days. In a subset of COCs the levels of phosphorylated Akt1 and ERK1/2 (MAPK1/3) were assessed at the 0th, 6th, 10th, 18th and 24th hours of in vitro maturation (IVM). At 18 and 24 h no difference existed in the proportion of matured oocytes in the ghrelin-treated group, while in the control group more (P < 0.05) matured oocyte were found at 24 h. Oocyte maturation for 24 h in the presence of ghrelin resulted in substantially reduced (P < 0.05) blastocyst yield(16.3%) in comparison with that obtained after 18 h (30.0%) or to both control groups (29.3% and 26.9%, for 18 and 24 h in maturation, respectively). Ghrelin-treated oocytes expressed lower Akt1 phosphorylation rate at the 10th hour of IVM, and higher ERK1/2 at the 6th and 10th hours of IVM compared with controls. In cumulus cells, at the 18th and 24th hours of IVM Akt1 phosphorylation rate was higher in ghrelin-treated oocytes. Our results imply that ghrelin acts in a different time-dependent manner on bovine oocytes and cumulus cells modulating Akt1 and ERK1/2 phosphorylation, which brings about acceleration of the oocyte maturation process.

Keywords

Akt1Cumulus cellsERK 1/2GhrelinIn vitro maturationOocyte
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 183 - 189
Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

5

These authors contributed equally to this work and are listed alphabetically.

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