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Effects of chemically defined medium on early development of porcine embryos derived from parthenogenetic activation and cloning

Published online by Cambridge University Press:  07 April 2011

Zubing Cao ,
Liucai Sui ,
Yunsheng Li ,
Suofei Ji ,
Xiaorong Zhang  and
Yunhai Zhang
Zubing Cao
Affiliation:
Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei city, Anhui Province 230036, China.
Liucai Sui
Affiliation:
Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei city, Anhui Province 230036, China.
Yunsheng Li
Affiliation:
Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei city, Anhui Province 230036, China.
Suofei Ji
Affiliation:
Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei city, Anhui Province 230036, China.
Xiaorong Zhang*
Affiliation:
130 Changjiang West Road, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Yunhai Zhang*
Affiliation:
130 Changjiang West Road, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
*
All correspondence to: Yunhai Zhang or Xiaorong Zhang. 130 Changjiang West Road, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Tel:/Fax: +86 551 5786357. e-mail: [email protected]
All correspondence to: Yunhai Zhang or Xiaorong Zhang. 130 Changjiang West Road, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Tel:/Fax: +86 551 5786357. e-mail: [email protected]
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Summary

The present study was to investigate if a completely chemically defined medium (PZM-4) could support the early development of porcine embryos derived from parthenogenetic activation (PA) and cloning (somatic cell nuclear transfer, SCNT), and to lay the foundation for determining the physiological roles of certain supplements in this medium. Porcine embryos derived from PA and SCNT were cultured in media: PZM-3 (a chemically semi-defined medium), PZM-4 (a fully defined medium), and PZM-5 (an undefined medium). Early embryo development was observed. We found that the three medium groups (PZM-3, PZM-4 and PZM-5) exhibited no significant differences in cleavage rates of PA embryos (p > 0.05), while the blastocyst rate in PZM-3 was significantly higher than in PZM-4 and PZM-5 (78.9% vs. 36.0% and 52.3%) (p < 0.05). Moreover, total cell number per blastocyst in PZM-3 was clearly higher than in PZM-5 but similar to that in PZM-4. As for SCNT embryos, no significant differences were observed for the cleavage rates or the blastocyst rates among the three groups (p > 0.05). However, total cell number per blastocyst in PZM-3 was notably higher than in PZM-5, but was similar to that in PZM-4. In conclusion, our results suggested that the completely chemically defined medium PZM-4 can be used to efficiently support the early development of porcine PA and SCNT embryos.

Keywords

Chemically defined mediumEmbryonic developmentIn vitro cultureSomatic cell nuclear transfer
Type
Research Article
Information
Zygote , Volume 20 , Issue 3 , August 2012 , pp. 229 - 236
Copyright
Copyright © Cambridge University Press 2011

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