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Effects of ascorbic acid on in vitro culture of bovine preantral follicles

Published online by Cambridge University Press:  05 April 2012

Evelyn R. Andrade ,
Robert van den Hurk ,
Lívia A. Lisboa ,
Mariana F. Hertel ,
Fabiana A. Melo-Sterza ,
Kleber Moreno ,
Ana Paula F. R. L. Bracarense ,
Fernanda C. Landim-Alvarenga ,
Marcelo M. Seneda  and
Amauri A. Alfieri
Evelyn R. Andrade*
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Robert van den Hurk
Affiliation:
Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Lívia A. Lisboa
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Mariana F. Hertel
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Fabiana A. Melo-Sterza
Affiliation:
Universidade Estadual do Mato Grosso do Sul, Aquidauna, MS, Brazil.
Kleber Moreno
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Ana Paula F. R. L. Bracarense
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Fernanda C. Landim-Alvarenga
Affiliation:
Universidade Estadual de São Paulo, Botucatu, SP, Brazil.
Marcelo M. Seneda
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
Amauri A. Alfieri
Affiliation:
Universidade Estadual de Londrina, Londrina, PR, Brazil.
*
All correspondence to Evelyn R. Andrade. Universidade Estadual de Londrina, Londrina, PR, Brazil. Tel: +55 4333015675. e-mail: [email protected]
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Summary

The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Compared with control fragments, the percentage of primordial follicles was reduced (p < 0.05) and the percentage of growing follicles had increased (p < 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (p < 0.05), but not when cultures were supplemented with 25, 50 or 100 μg/ml of ascorbic acid. Ultrastructural and immunohistochemical analysis of 8 day cultured ovarian cortical fragments, however, showed the integrity and viability of follicles only when fragments were cultured in presence of 50 μg/ml of ascorbic acid. In conclusion, this study demonstrated that addition of ascorbic acid to MEM at a concentration of 50 μg/ml not only stimulates the activation of 8 day in vitro cultured cattle primordial follicles and subsequent growth of activated follicles, but also safeguards the viability of these early-stage follicles.

Keywords

Ascorbic acidCattleIn vitro culturePreantral follicles
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 379 - 388
Copyright
Copyright © Cambridge University Press 2012

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