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Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera

Published online by Cambridge University Press:  02 May 2012

M.C. Gramajo-Bühler ,
F. Pucci-Alcaide  and
G. Sánchez-Toranzo
M.C. Gramajo-Bühler*
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
F. Pucci-Alcaide
Affiliation:
Fundación Miguel Lillo Instituto de Morfología Aninal IMA, Miguel Lillo 251, San Miguel de Tucumán 4000, Tucumán, Argentina.
G. Sánchez-Toranzo
Affiliation:
Departamento de Biología del Desarrollo INSIBIO, Chacabuco 461, San Miguel de Tucumán 4000, Tucumán, Argentina.
*
All correspondence to: María Cecilia Gramajo-Bühler. Departamento de Biología del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina. Tel: +54 381 4247752 int. 7093. Fax: +54 381 4247752 int. 7004.
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Summary

Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen–thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material.

Keywords

Epididymal spermChinchilla lanigeraCryopreservationDNA fragmentationSperm quality
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 351 - 357
Copyright
Copyright © Cambridge University Press 2012 

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References

Amann, R.P. & Pickett, B.W. (1987). Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J. Equine Vet. Sci. 7, 145–73.CrossRefGoogle Scholar
Bunge, R.G. & Sherman, J.K. (1953). Fertilizing capacity of frozen human spermatozoa. Nature 172, 767.CrossRefGoogle ScholarPubMed
Caiza de la Cueva, F.I., Rigau, T., Bonet, S., Mire, J., Briz, M. & Rodriguez-Gil, J.E. (1997). Subjecting horse spermatozoa to hypoosmotic incubation: effects of ouabain. Theriogenology 47, 765–84.CrossRefGoogle Scholar
Carrascosa, R.E., Martin, A.C., Ponzio, M.F., Busso, J.M., Ponce, A.A. & Lacuara, J.L. (2001). Storage of Chinchilla lanigera semen at 4°C for 24 or 72 h with two different cryoprotectants. Cryobiology 42, 301–6.CrossRefGoogle ScholarPubMed
Correa, J.R. & Zavos, P.M. (1995). Frozen–thawed bovine spermatozoa diluted by slow or rapid dilution method: measurements on occurrence of osmotic shock and sperm viability. Theriogenology 44, 963–71.CrossRefGoogle ScholarPubMed
Critser, J.K., Huse-Benda, A.R., Aaker, D.V., Arenson, B.W. & Bal, G.D. (1988). Cryopreservation of human spermatozoa. III. The effect of cryoprotectants on motility. Fertil. Steril. 50, 314–20.CrossRefGoogle ScholarPubMed
Curry, M.R. (2000). Cryopreservation of semen from domestic livestock. J. Reprod. Fertil. 5, 46–52.Google ScholarPubMed
Foulkes, J.A. & Stewart, D.L. (1977). Fertility of dairy cattle after artificial insemination with semen frozen in a lipoprotein diluents. J. Reprod. Fertil. 51, 175–7.CrossRefGoogle Scholar
Gade, A.J., Matas, C. & Lucas, X. (1998). Prediction of porcine semen fertility by homologous in vitro penetration (HIVP) assay. Anim. Reprod. Sci. 54, 95–108.CrossRefGoogle Scholar
Hammadeh, M.E., Greiner, S., Rosenbaum, P. & Schmidt, W. (2001). Comparison between human sperm preservation medium and test-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze–thawing procedure. J. Androl. 22, 1012–8.CrossRefGoogle ScholarPubMed
Hammerstedt, R.H. & Graham, J.K. (1992). Cryopreservation of poultry sperm: the enigma of glycerol. Cryobiology 29, 26–38.CrossRefGoogle ScholarPubMed
Holt, W.V., Head, M.F. & North, R.D. (1992) Freeze-induced membrane damage in ram spermatozoa is manifested after thawing: observations with experimental cryomicroscopy. Biol. Reprod. 46, 1086–94.CrossRefGoogle ScholarPubMed
Hossain, A., Islam, N., Caruso, A. & Madanes, A. The Midwest Fertility Ctr, Downers Grove, IL (2003). Human sperm cryopreservation using sucrose as a cryoprotectant shows a promising future. Fertil. Steril. 80, 359, S239. Abstr.CrossRefGoogle Scholar
Jeyendran, R.S., Van der Ven, HH, Perez-Pelaez, M., Crabo, B.G. & Zaneveld, L.J. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70, 219–28.CrossRefGoogle ScholarPubMed
Kukis, A. Yolk lipids. (1992). Biochimbiophysacta 1124, 205–22.Google Scholar
Kumi-Diaca, J. (1993). Subjecting canine semen to the hypoosmotic test. Theriogenology 39, 1279–89.CrossRefGoogle Scholar
Martins, C.F., Dode, M.N., Báo, S.N. & Rumpf, R. (2007). The use of the acridine orange test and the tunel assay to assess the integrity of freeze-dried bovine spermatozoa DNA. Genet. Mol. Res. 6, 94–104.Google ScholarPubMed
Nakagata, N. (1993). Production of normal young following transfer of mouse embryos obtained by in vitro fertilization between cryopreserved gametes. J. Reprod. Fertil. 99, 77–80.CrossRefGoogle ScholarPubMed
Neild, D., Chaves, G., Flores, M., Mora, N., Beconi, M. & Aguero, A. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology 51, 72l–7.CrossRefGoogle ScholarPubMed
Nie, G.J. (1998). Development of a hypoosmotic swelling (HOS) test for stallion semen. Proc. Sot. Theriogen Baltimore MD, 146 Abstr.Google Scholar
Parkinson, L.R. (1987). Artificial insemination. In Rancher's Handbook.10th edition.Colorado, USA. Empress Chinchilla Breeders Cooperative Inc. File 50.00–50.20.Google Scholar
Perez, L.J., Valcarcel, A., de las Hems, M.A. & Baldassarre, H. (1997). Comparative study of four techniques for evaluation of sperm quality in ovine and bovine frozen-thawed samples. Reprod. Dom. Anim. 32, 157–60.CrossRefGoogle Scholar
Polge, C., Smith, A.U. & Parks, A.S. (1949). Survival of spermatozoa after vitrification and dehydration at low temperatures. Nature 164, 666–76.CrossRefGoogle Scholar
Ponce, A.A., Aires, V.A., Carrascosa, R., Fiol De Cuneo, M., Ruiz, R.D. & Lacuara, J.L. (1998a). Functional activity of epididymal Chinchilla lanigera spermatozoa cryopreserved in different extenders. R. Vet. Sci. 64, 239–43.CrossRefGoogle Scholar
Ponce, A.A., Carrascosa, R., Aires, V.A., Fiol De Cuneo, M., Ruiz, R.D., Ponzio, M.F. & Lacuara, J.L. (1998b). Activity of Chinchilla laniger spermatozoa collected by electroejaculation and cryopreserved. Theriogenology 50, 1239–49.CrossRefGoogle ScholarPubMed
Prins, G.S. & Weidel, L. (1986). A comparative study of buffer system as cryoprotectants for human spermatozoa. Fertil. Steril. 46, 147–9.CrossRefGoogle ScholarPubMed
Rota, A., Penzo, N., Vincent, L. & Mantovani, R. (2000). Hypoosmotic swelling (HOS) as a screening assay for testing in vitro fertility of bovine spermatozoa. Theriogenology 53, 1416–20.CrossRefGoogle Scholar
Royere, D., Barthelemy, C., Hamamah, S. & Lansac, J. (1996). Cryopreservation of spermatozoa: a 1996 review. Hum. Reprod. Update 2, 553–9.CrossRefGoogle ScholarPubMed
Seier, J.V., Conredie, E., Oettle, E.E. & Fincham, J.E. (1993). Cryopreservation of vervet monkey semen and recovery of progressively motile spermatozoa. J. Med. Primatol. 22, 355–9.CrossRefGoogle ScholarPubMed
Stanic, P., Tandara, M., Sonicki, Z., Simunic, V., Radakovic, B. & Suchanek, E. (2000). Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur. J. Obstet. Gynecol. Reprod. Biol. 91, 65–70.CrossRefGoogle ScholarPubMed
Weidel, L. & Prins, G.S. (1987). Cryosurvival of human spermatozoa frozen in eight different buffer systems. J. Androl. 8, 41–7.CrossRefGoogle ScholarPubMed
World Health Organization. (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen – 5th Edn.Nonserial publications.Google Scholar
Yoshida, M., Forsberg, M., Greve, T., Gustafsson, H., Katila, T., Kindahl, H & Ropstad, E. (2000). Conservation of sperm: current status and new trends. Anim. Reprod. Sci. 60–61, 349–55.CrossRefGoogle ScholarPubMed