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Effect of zinc and copper on preimplantation mouse embryo development in vitro and metallothionein levels

Published online by Cambridge University Press:  26 September 2008

Francesca Vidal  and
Juan Hidalgo
Francesca Vidal
Affiliation:
Autonomous University of Barcelona, Barcelona, Spain
Juan Hidalgo*
Affiliation:
Autonomous University of Barcelona, Barcelona, Spain
*
Juan Hidalgo, Departamento de Biologia Celular y Fisiologia, Unidad de Fisiologia Animal, Facultad de Ciencias, Universidad Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain. Fax: (3) 581 22 95.
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Extract

The effect of zinc and copper on the in vitro development of mouse preimplantation embryos and on metallothionein (MT) levels was studied by exposing the embryos to 100 μM concentrations of the metals for 24 h at the 1-cell,2-cell, 6-8-cell, morula and blastocyst stages. Zinc affected embryo development in the early but not in the late stages, whereas copper affected it more generally. The combined presence of both metals caused a stronger embryotoxicity. MT levels were measured by radioimmunoassay and were found to be similar at all developmental stages, though possibly higher at the blastocyst stage. The exposure of embryos to zinc and copper increased MT levels significantly only at the blastocyst stage, supporting previously published results on MT mRNA levels.

Keywords

CopperEmbryoMetallothioneinZinc
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 225 - 229
Copyright
Copyright © Cambridge University Press 1993

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References

Andrews, G.K., Huet, Y.M., Lehman, L.D. & Dey, S.K. (1987). Metallothionein gene regulation in the preimplantation rabbit blastocyst.. Development 100, 463–9.CrossRefGoogle ScholarPubMed
Andrews, G.K., Huet-Hudson, Y.M., Paria, B.C.et al. (1991). Metallothionein gene expression and metal regulation during preimplantation mouse embryo development (MT mRNA during early development). Dev. Biol. 145, 13–27.CrossRefGoogle ScholarPubMed
Angerer, L.M., Kawczynski, G., Wilkinson, D.G.et al. (1986). Spatial patterns of metallothionein mRNA expression in the sea urchin embryo. Dev.Biol. 116,543–7.CrossRefGoogle ScholarPubMed
Bremner, I. (1991). Nutritional and physiological significance of metallothionein. Methods Enzymol. 205, 25–35.CrossRefGoogle ScholarPubMed
Gasull, T., Rebollo, D.V., Romero, B. & Hidalgo, J. (1993). Development of a competitive double antibody radioimmunoassay for rat metallothionein. J. Immunoassay (in press).CrossRefGoogle ScholarPubMed
Hidalgo, J., Campmany, L., Borras, M.et al. (1988a). Metallothionein response to stress in rats: role in free radical scavenging. Am. J. Physiol. 255, E518–E524.Google ScholarPubMed
Hidalgo, J., Giralt, M., Garvey, J.S. & Armario, A. (1988b). physiological role of glucocorticoids on rat serum and liver metallothionein in basal and stress conditions. Am. J. Physiol. 254. E71–E78.Google ScholarPubMed
Hogan, B., Constantini, F. & Lacy, E. (1986). Summary of mouse development. In: Manipulating the Mouse Embryo. pp.17–71. New york: Cold Spring Harbor Laboratory.Google Scholar
Kägi, J.H.R. & Kojima, Y. (eds.) (1987). Metallothionein II. Basel: Birkhäuser.CrossRefGoogle Scholar
Nemer, M., Travaglini, E.C., Rondinelli, E. & D'Alonzo, J. (1984). Developmental regulation induction, and embryonic tissue specificity of sea urchin metallothionein gene expression. Dev. Biol. 102, 471–82.CrossRefGoogle ScholarPubMed
Quinn, P., Barros, C. & Whittingham, D.G. (1982). Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa. Reprod. Fert.. 66,161–8.CrossRefGoogle ScholarPubMed
Templeton, D.M. & Cherian, M.G. (1991). Toxicological significance of metallothionein. Methods Enzymol. 205, 11–24.CrossRefGoogle ScholarPubMed
Webb, M. (1987). Metallothionein in regeneration, reproduction and development. In: Metallothionein II, ed. Kägi, J.H.R. & Kojima, Y., pp. 483–98.Basel: Birkhäuser.CrossRefGoogle Scholar
Whitten, W.K. & Biggers, J.D. (1968). Complete culture of the pre-implantational stages of the mouse in vitro. J. Reprod. Fert. 17, 399–401.CrossRefGoogle Scholar
Whittingham, D.G. (1971). Culture of mouse ova J. Reprod. Fert. (Suppl.) 14,7–21.Google ScholarPubMed