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The effect of the time interval between injection and parthenogenetic activation on the spindle formation and the in vitro developmental potential of somatic cell nuclear-transferred rat oocytes

Published online by Cambridge University Press:  14 August 2009

Shigetoshi Mizumoto ,
Yoko Kato  and
Yukio Tsunoda
Shigetoshi Mizumoto
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631–8505, Japan.
Yoko Kato
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631–8505, Japan.
Yukio Tsunoda*
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631–8505, Japan. Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631–8505, Japan.
*
All correspondence to: Yukio Tsunoda. Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631–8505, Japan. Tel +81 742 43 5143. Fax: +81 742 43 5393. e-mail: [email protected]
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Summary

We examined the optimal conditions for somatic cell nuclear transfer (SCNT) in the rat. First, we examined the effect of preincubation time before activation on SCNT rat oocytes produced in the presence of MG132 with regard to spindle formation and the potential to develop into blastocysts. The spindles of SCNT oocytes continued to elongate with an increase in the culture duration and, in approximately half of oocytes, the chromosomes were distributed along the spindles at 120 min after incubation. Such abnormal spindle formation in SCNT oocytes is a possible reason for the low developmental potential of SCNT rat oocytes. To inhibit the formation of abnormal spindle formation, we examined secondly the developmental potential of rat SCNT oocytes that had been preincubated with nocodazole and demecolcine instead of MG132. The developmental rates in SCNT oocytes, however, were decreased. For successful rat somatic cell cloning, two steps might be required: (1) to culture the somatic cell nuclei for a sufficient time in MII oocyte cytoplasm to enhance nuclear reprogramming; and (2) to induce normal spindle formation with normal chromosomal construction.

Keywords

DemecolcineMG132NocodazoleNuclear transferParthenogenesisTSA
Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 9 - 15
Copyright
Copyright © Cambridge University Press 2009

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References

Campbell, K.H.S., Fisher, P., Chen, W.C., Choi, I., Kelly, R.D., Lee, J.H. & Xhu, J. (2007). Somatic cell nuclear transfer: past, present and future perspectives. Theriogenology 68S, S214–37.Google Scholar
Ding, X., Wang, Y., Zhang, D., Wang, Y., Guo, Z. & Zhang, Y. (2008). Increased pre-implantation development of cloned bovine embryos treated with 5-aza-20-deoxycytidine and trichostatin A. Theriogenology 70, 622–30.Google Scholar
Du, F., Sung, L.Y., Tian, X.C. & Yang, X. (2002). Differential cytoplast requirement for embryonic and somatic cell nuclear transfer in cattle. Mol. Reprod. Dev. 63, 183–91.CrossRefGoogle ScholarPubMed
Ehrhardt, A.G. & Sluder, G. (2005). Spindle pole fragmentation due to proteasome inhibition. J. Cell. Physiol. 204, 808–18.CrossRefGoogle ScholarPubMed
Erbach, G.T., Lawitts, J.A., Papaioannou, V.E. & Biggers, J.D. (1994). Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod. 50, 1027–33.CrossRefGoogle ScholarPubMed
Futon, B.P., & Whittingham, D.G. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle Scholar
Hayes, E., Galea, S., Verkuylen, A., Pera, M., Morrison, J., Lacham-Kaplan, O. & Trounson, A. (2001). Nuclear transfer of adult and genetically modified fetal cells of the rat. Physiol. Genomics 5, 193–204.CrossRefGoogle ScholarPubMed
Hirabayashi, M., Kato, M., Ishikawa, A. & Hochi, S. (2003). Factors influencing chromosome condensation and development of cloned rat embryos. Cloning Stem Cells 5, 35–42.CrossRefGoogle ScholarPubMed
Iannaccone, P., Taborn, G. & Garton, R. (2001). Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote 9, 135–43.CrossRefGoogle Scholar
Ito, J., Hirabayashi, M., Kato, M., Takeuchi, A., Ito, M., Shimada, M. & Hochi, S. (2005). Contribution of high p35cdc kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes. Reproduction 129, 171–80.CrossRefGoogle ScholarPubMed
Jiang, J.Y., Mizuno, S., Mizutani, E., Sasada, H. & Sato, E. (2002). Parthenogenetic activation and subsequent development of rat oocytes in vitro. Mol. Reprod. Dev. 61, 120–5.Google Scholar
Kato, M., Hirabayashi, M., Aoto, T., Ito, K., Ueda, M. & Hochi, S. (2001). Strontium-induced activation regimen for rat oocytes in somatic cell nuclear transplantation. J. Reprod. Dev. 47, 407–13.Google Scholar
Kato, Y. & Tsunoda, Y. (1992). Synchronous division of mouse two-cell embryos with nocodazole in vitro. J. Reprod. Fertil. 95, 39–43.CrossRefGoogle ScholarPubMed
Kawakami, M., Tani, T., Yabuuchi, A., Kobayashi, T., Murakami, H., Fujimura, T., Kato, Y. & Tsunoda, Y. (2003). Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes. Cloning Stem Cells 5, 379–87.CrossRefGoogle ScholarPubMed
Keefer, C.L. & Schuetz, A.W. (1982). Spontaneous activation of ovulated rat oocytes during in vitro culture. J. Exp. Zool. 224, 371–77.CrossRefGoogle ScholarPubMed
Kishigami, S., Wakayama, S., Thuan, N.V., Ohta, H., Mizutani, E., Hikichi, T., Bui, H.T., Balbach, S., Ogura, A., Boiani, M. & Wakayama, T. (2006a). Production of cloned mice by somatic cell nuclear transfer. Nat. Protoc. 1, 125–38.CrossRefGoogle ScholarPubMed
Kishigami, S., Mizutani, E., Ohta, H., Hikichi, T., Thuan, N.V., Wakayama, S., Bui, H.T. & Wakayama, T. (2006b). Significant improvement of mouse cloning technique by treatment with trichostatin A. Biochem. Biophys. Res. Commun. 340, 183–9.CrossRefGoogle ScholarPubMed
Kubiak, J.Z., Weber, M., de Pennart, H., Winston, N.J. & Maro, B. (1993). The metaphase II arrest in mouse oocytes is controlled through microtubule-dependent destruction of cyclin B in the presence of CSF. EMBO J. 12, 3773–8.CrossRefGoogle ScholarPubMed
Lee, J.H. & Campbell, K.H.S. (2006). Effects of enucleation and caffeine on maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK) activities in ovine oocytes used as recipient cytoplasts for nuclear transfer. Biol. Reprod. 74, 691–8.CrossRefGoogle ScholarPubMed
Miyoshi, K., Kono, T. & Niwa, K. (1997). Stage-dependent development of rat 1-cell embryos in a chemically defined medium after fertilization in vivo and in vitro. Biol. Reprod. 56, 180–5.Google Scholar
Mizumoto, S., Kato, Y. & Tsunoda, Y. (2008). The developmental potential of parthenogenetic and somatic cell nuclear-transferred rat oocytes in vitro. Cloning Stem Cells 10, 453–9.Google Scholar
Mizutani, E., Jiang, J-Y., Mizuno, S., Tomioka, I., Shinozawa, T., Kobayashi, J., Sasada, H. & Sato, E. (2004). Determination of optimal concentrations for parthenogenetic activation and subsequent development of rat oocytes in vitro. J. Reprod. Dev. 50, 139–46.Google Scholar
Popova, E., Bader, M. & Krivokharchenko, A. (2009). Efficient production of nuclear transferred rat embryos by modified methods of reconstruction. Mol. Reprod. Dev. 76, 208–16.CrossRefGoogle ScholarPubMed
Rybouchkin, A., Kato, Y. & Tsunoda, Y. (2006). Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74, 1083–9.Google Scholar
Sung, LY., Shen, PC., Jeong, B.S., Xu, J., Chang, C.C., Cheng, W.T., Wu, J.S., Lee, S.N., Broek, D., Faber, D., Tian, X.C., Yang, X. & Du, F. (2007). Premature chromosome condensation is not essential for nuclear reprogramming in bovine somatic cell nuclear transfer. Biol. Reprod. 76, 232–40.Google Scholar
Tani, T., Kato, Y. & Tsunoda, Y. (2001). Direct expose of chromosomes to nonactivated ovum cytoplasm is effective for bovine somatic cell nucleus reprogramming. Biol. Reprod. 64, 324–30.CrossRefGoogle Scholar
Tani, T., Kato, Y. & Tsunoda, Y. (2003). Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity. Biol Reprod. 69, 1890–4.Google Scholar
Tani, T., Shimada, H., Kato, Y. & Tsunoda, Y. (2006). Demecolcine assisted enucleation for bovine cloning. Cloning Stem Cells 1, 61–6.CrossRefGoogle Scholar
Tani, T., Shimada, H., Kato, Y. & Tsunoda, Y. (2007). Bovine oocytes with the potential to reprogram somatic cell nuclei have a unique 23-kDa protein, phosphorylated transcriptionally controlled tumor protein (TCTP). Cloning Stem Cells 9, 267–80.Google Scholar
Tomioka, I., Mizutani, E., Yoshida, T., Sugawara, A., Inai, K., Sasada, H. & Sato, E. (2007). Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer. J. Reprod. Dev. 53, 835–42.CrossRefGoogle ScholarPubMed
Wakayama, T., Perry, A.C., Zuccotti, M., Johnson, K.R. & Yanagimachi, R. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394, 369–74.Google Scholar
Wilmut, I., Schnieke, A.E., McWhir, J., Kind, A.J. & Campbell, K.H.S. (1997). Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–3.Google Scholar
Yin, X.J., Tani, T., Yonemura, I., Kawakami, M., Miyamoto, K., Hasegawa, R., Kato, Y. & Tsunoda, Y. (2002). Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes. Biol. Reprod. 67, 442–6.Google Scholar
Zhang, Y., Li, J., Villemoes, K., Pedersen, A.M., Purup, S. & Vajta, G. (2007). An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer. Cloning Stem Cells 9, 357–63.Google Scholar
Zhou, Q., Renard, J-P., Le Friec, G., Brochard, V., Beaujean, N., Cherifi, Y., Fraichard, A. & Cozzi, J. (2003). Generation of fertile cloned rats by regulating oocyte activation. Science 302, 1179.CrossRefGoogle ScholarPubMed