Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-29T01:32:17.708Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of sperm preparation on development of bovine blastocyst in vitro

Published online by Cambridge University Press:  01 November 2016

Maria Celina Abraham ,
Anders Johannisson  and
Jane M. Morrell
Maria Celina Abraham*
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
Anders Johannisson
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
Jane M. Morrell
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
*
All correspondence to Maria Celina Abraham. Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden. Tel: +46 01867 11 62. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Sperm preparation is an important step in the in vitro production of embryos. Centrifugation through colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B). Computer-assisted sperm motility analysis, the hypo-osmotic swelling test, and the sperm chromatin structure assay were performed on unselected (control) and SLC-selected sperm samples. Mini and Mini-EP gave the highest yield of motile spermatozoa, progressive motility and membrane integrity. In vitro fertilization trials were performed to investigate the fertilizing ability of the frozen–thawed bovine spermatozoa selected with Bovicoll. Mini-SLC (single-layer centrifugation) and swim-up (Control) were performed and cleavage rate and blastocyst rate did not differ significantly between groups. As there was a trend to an increased number of cells in blastocysts in the SLC group, the Mini-SLC method is at least as good as swim-up for selecting frozen–thawed bull spermatozoa for in vitro fertilization (IVF). This method could potentially be used to prepare human sperm for assisted reproduction.

Keywords

BovineComputer-assisted semen analysis (CASA)In vitro fertilization (IVF)In vitro production (IVP) of embryosSingle-layer centrifugationSperm chromatin structure assay (SCSA)
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 825 - 830
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abraham, M.C., Gustafsson, H., Ruete, A. & Brandt, Y. (2012). Breed influences on in vitro development of abattoir-derived bovine oocytes. Acta. Vet. Scand. 54, 36.CrossRefGoogle ScholarPubMed
Anel-López, L., Martínez-Rodríguez, C., Soler, A.J., Fernández-Santos, M.R., Garde, J.J. & Morrell, J.M. (2015). Use of Androcoll-S after thawing improves the quality of electroejaculated and epididymal sperm samples from red deer. Anim. Reprod. Sci. 158, 68–74.CrossRefGoogle ScholarPubMed
Chianese, R., Chioccarelli, T., Cacciola, G., Ciaramella, V., Fasano, S., Pierantoni, R., Meccariello, R. & Cobellis, G. (2011). The contribution of lower vertebrate animal models in human reproduction research. Gen. Comp. Endocr. 171, 17–27.CrossRefGoogle ScholarPubMed
Correa, J.R. & Zavos, P.M. (1994). The hypoosmotic swelling test: its employment as an assay to evaluate the functional integrity of the frozen–thawed bovine sperm membrane. Theriogenology 42, 351–60.Google Scholar
Evenson, D. & Jost, L. (2000). Sperm chromatin assay is useful for fertility assessment. Methods Cell Sci. 22, 169–89.Google Scholar
Goodhand, K.L., Staines, M.E., Hutchinson, J.S.M. & Broadbent, P.J. (2000). In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH. Anim. Reprod. Sci. 63, 145–58.Google Scholar
Goodla, L., Morrell, J.M., Yusnizar, Y., Stålhammar, H. & Johannisson, A. (2014). Quality of bull spermatozoa after preparation by single-layer centrifugation. J. Dairy Sci. 97, 2204–12.Google Scholar
Hansen, C., Vermeiden, T., Vermeiden, J. P. W., Simmet, C., Day, B. C. & Feitsma, H. (2006). Comparison of FACSCount AF system, improved Neubauer hemocytometer, Corning 254 photometer, SpermVision, UltiMate and NucleoCounter SP-100 for determination of sperm concentration of boar semen. Theriogenology 66, 2188–94.Google Scholar
Jiménez-Rabadán, P., Morrell, J.M., Johannisson, A., Ramón, M., García-Álvarez, O., Maroto-Morales, A., Álvaro-García, P., Pérez-Guzmán, M., Fernández-Santos, M. & Garde, J. (2012). Single-layer centrifugation (SLC) improves sperm quality of cryopreserved Blanca-Celtibérica buck semen. Anim. Reprod. Sci. 136, 47–54.Google Scholar
Kuwayama, M. (2005). Highly efficient vitrification method for cryopreservation of human oocytes. RBM Online 11, 300–8.Google Scholar
Lindner, G.M. & Wright, R.W. (1983). Bovine embryo morphology and evaluation. Theriogenology 20, 407–16.Google Scholar
Morrell, J.M., Moffatt, O., Sakkas, D., Manicardi, G.C., Bizzaro, D., Tomlinson, M., Nilsson, H. & Holmes, P.V. (2004). reduced senescence and retained nuclear dna integrity in human spermatozoa prepared by density gradient centrifugation. J. Assist. Reprod. Genet. 21, 217–22.CrossRefGoogle ScholarPubMed
Morrell, J.M., Dalin, A.M. & Rodriguez-Martinez, H. (2009). Comparison of density gradient and single-layer centrifugation of stallion spermatozoa: Yield, motility and survival. Equine Vet. J. 41, 53–8.Google Scholar
Morrell, J.M. & Rodriguez-Martinez, H. (2011). Practical applications of sperm selection techniques as a tool for improving reproductive efficiency. Vet. Med. Int. 2011, 1–9.CrossRefGoogle Scholar
Muñoz-Fuentes, V., Linde Forsberg, C., Vilà, C. & Morrell, J.M. (2014). Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from grey wolves, Canis lupus (L.). Theriogenology 82, 773–6.CrossRefGoogle ScholarPubMed
R Development Core Team. (2014). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria.Google Scholar
Samardzija, M., Karadjole, M., Getz, I., Makek, Z., Cergolj, M. & Dobranic, T. (2006). Effects of bovine spermatozoa preparation on embryonic development in vitro . Reprod. Biol. Endocrinol. 4, 58.Google Scholar
Takahashi, Y. & First, N.L. (1992). In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37, 963–78.CrossRefGoogle ScholarPubMed
Thys, L., Vandaele, L., Morrell, J.M., Mestach, J., Van Soom, A., Hoogewijs, M. & Rodriguez-Martinez, H. (2009). In vitro fertilizing capacity of frozen–thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation. Reprod. Domestic Anim. 44, 390–4.Google Scholar
Trasorras, V., Giuliano, S., Chaves, G., Neild, D., Agüero, A., Carretero, M., Pinto, M., Baca Castex, C., Alonso, A., Rodriguez, D., Morrell, J.M. & Miragaya, M. (2012). In vitro embryo production in llamas (Lama glama) from in vivo matured oocytes with raw semen processed with Androcoll-e using defined embryo culture media. Reprod. Domestic Anim. 47, 562–567.CrossRefGoogle ScholarPubMed
World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen. WHO: Geneva, Switzerland.Google Scholar
Yulnawati, Y., Abraham, M.C., Laskowski, D., Johannisson, A. & Morrell, J.M. (2014). Changes in bull sperm kinematics after single-layer centrifugation. Reprod. Dom. Anim. 49, 954–6.Google Scholar