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Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro

Published online by Cambridge University Press:  16 September 2013

Mohammad Salehi ,
Yoko Kato  and
Yukio Tsunoda
Mohammad Salehi
Affiliation:
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Yoko Kato*
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.
Yukio Tsunoda
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.
*
All correspondence to: Yoko Kato. Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan. Tel: +81 742 43 5393. Fax: +81 742 43 5393. e-mail: [email protected]
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Summary

The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on mouse somatic cell nuclear transfer remains unknown. In this study, we assessed the effects of various concentrations of melatonin (10−6 to 10−12 M) on the in vitro development of mouse somatic cell nuclear transfer embryos for 96 h. Embryos cultured without melatonin were used as control. There was no significant difference in cleavage rates between the groups supplemented with melatonin, dimethyl sulphoxide (DMSO) and the control. The rate of development to blastocyst stage was significantly higher in the group supplemented with 10−12 M melatonin compared with the control group (P < 0.05). Thus, our data demonstrated that adding melatonin to pre-implantation mouse nuclear-transferred embryos can accelerate blastocyst formation.

Keywords

MelatoninMouse somatic cell nuclear transferReactive oxygen species
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 213 - 217
Copyright
Copyright © Cambridge University Press 2013 

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