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Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa

Published online by Cambridge University Press:  29 October 2012

Alexander V. Makarevich ,
Eliska Spalekova ,
Lucia Olexikova ,
Elena Kubovicova  and
Zdenka Hegedusova
Alexander V. Makarevich*
Affiliation:
Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 Lužianky near Nitra, Slovak Republic.
Eliska Spalekova
Affiliation:
Slovak Agricultural University, 94901 Nitra, Slovak Republic.
Lucia Olexikova
Affiliation:
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic.
Elena Kubovicova
Affiliation:
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic.
Zdenka Hegedusova
Affiliation:
Research Institute for Cattle Breeding Ltd. Rapotin, Vyzkumniku 267, 788 13 Vikyrovice, Czech Republic.
*
All correspondence to: Alexander V. Makarevich, Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 Lužianky near Nitra, Slovak Republic. e-mail: [email protected]
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Summary

The aim of the study was to examine the effects of insulin-like growth factor I (IGF-I) on ram sperm traits after hypothermic storage. Sperm ejaculates from Lacaune rams were diluted in a Tris extender, pooled, divided into groups of IGF-I doses tested (0, 10, 100 or 200 ng.ml−1) and stored (0–5°C) for 96 h. IGF-I elevated whole sperm motility as measured by a Computer-assisted Sperm Analyser (CASA) system, by 24 h (10 ng.ml−1) and 48 h (200 ng.ml−1) of storage, and by progressive movement on each day of storage. After 72 h the sperm samples were analysed for plasma membrane integrity (peanut agglutinin–fluorescein isothiocyanate), membrane stability (annexin V–Fluos) and apoptosis (Yo-Pro®-1) using fluorescence microscopy. The addition of IGF-I (at 100 or 200 ng.ml−1) reduced the ratio of sperm with disrupted membranes and the ratio of annexin V-labelled sperm. The ratio of apoptotic sperm was reduced by IGF-I given at 10 or 100 ng.ml−1 compared with control. Sperm fertilizing ability, determined at 48 h by an in vitro fertilization (IVF) test on bovine oocytes, was increased by IGF-I given at 100 ng.ml−1 from 47.0 to 67.7%. In conclusion, IGF-I maintained ram sperm functions following cooling storage and its effects were reflected in sperm fertilizing ability in vitro.

Keywords

Fertilizing abilityIGF-IMotilitySpermViability
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 305 - 313
Copyright
Copyright © Cambridge University Press 2012 

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