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Effect of cryopreservation and in vitro culture of bovine fibroblasts on histone acetylation levels and in vitro development of hand-made cloned embryos

Published online by Cambridge University Press:  07 July 2010

Liliana Chacón ,
Martha C. Gómez ,
Jill A. Jenkins ,
Staley P. Leibo ,
Gemechu Wirtu ,
Betsy L. Dresser  and
C. Earle Pope
Liliana Chacón
Affiliation:
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA. School of Veterinarian Medicine, Colombian National University, Bogotá, Colombia.
Martha C. Gómez*
Affiliation:
Audubon Center for Research of Endangered Species, 14001 River Road, New Orleans, Louisiana, USA. Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA.
Jill A. Jenkins
Affiliation:
National Wetlands Research Center, US Geological Survey, Lafayette, Louisiana, USA.
Staley P. Leibo
Affiliation:
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA. Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana, USA.
Gemechu Wirtu
Affiliation:
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA.
Betsy L. Dresser
Affiliation:
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA. Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana, USA.
C. Earle Pope
Affiliation:
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA.
*
All correspondence to: Martha C. Gómez. Audubon Center for Research of Endangered Species, 14001 River Road, New Orleans, Louisiana, USA. Tel: +504 398 3159. Fax: +504 391 7707. e-mail: [email protected]
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Summary

In this study, the relative acetylation levels of histone 3 in lysine 9 (H3K9ac) in cultured and cryopreserved bovine fibroblasts was measured and we determined the influence of the epigenetic status of three cultured (C1, C2 and C3) donor cell lines on the in vitro development of reconstructed bovine embryos. Results showed that cryopreservation did not alter the overall acetylation levels of H3K9 in bovine fibroblasts analysed immediately after thawing (frozen/thawed) compared with fibroblasts cultured for a period of time after thawing. However, reduced cleavage rates were noted in embryos reconstructed with fibroblasts used immediately after thawing. Cell passage affects the levels of H3K9ac in bovine fibroblasts, decreasing after P1 and donor cells with lower H3K9ac produced a greater frequency of embryo development to the blastocyst stage. Cryopreservation did not influence the total cell and ICM numbers, or the ICM/TPD ratios of reconstructed embryos. However, the genetic source of donor cells did influence the total number of cells and the trophectoderm cell numbers, and the cell passage influenced the total ICM cell numbers.

Keywords

BovineEmbryoFibroblastsHistone acetylationSCNT
Type
Research Article
Information
Zygote , Volume 19 , Issue 3 , August 2011 , pp. 255 - 264
Copyright
Copyright © Cambridge University Press 2010

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