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Effect of butafosfan supplementation during oocyte maturation on bovine embryo development

Published online by Cambridge University Press:  15 August 2019

Lucas Teixeira Hax ,
Joao Alveiro Alvarado Rincón Open the ORCID record for Joao Alveiro Alvarado Rincón [Opens in a new window] ,
Augusto Schneider ,
Lígia Margareth Cantarelli Pegoraro ,
Letícia Franco Collares ,
Rubens Alves Pereira ,
Jorgea Pradieé ,
Francisco Augusto Burket Del Pino  and
Marcio Nunes Corrêa
Lucas Teixeira Hax
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Joao Alveiro Alvarado Rincón
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Augusto Schneider
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Lígia Margareth Cantarelli Pegoraro
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Pelotas, RS, 96010-971, Brazil
Letícia Franco Collares
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Rubens Alves Pereira
Affiliation:
Laboratório Ibasa, Porto Alegre, RS, 90220-030, Brazil
Jorgea Pradieé
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Francisco Augusto Burket Del Pino
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
Marcio Nunes Corrêa*
Affiliation:
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil
*
Address for correspondence: Marcio Nunes Corrêa, Departamento de Clínicas Veterinária, Universidade Federal de Pelotas, Campus capão do Leão, CEP: 96010–000 Pelotas, RS – Brazil. Tel: +55 53 32757136. E-mail: má[email protected]
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Summary

Around 60–80% of oocytes maturated in vivo reached competence, while the proportion of maturation in vitro is rarely higher than 40%. In this sense, butafosfan has been used in vivo to improve metabolic condition of postpartum cows, and can represent an alternative to increase reproductive efficiency in cows. The aim of this study was to evaluate the addition of increasing doses of butafosfan during oocyte maturation in vitro on the initial embryo development in cattle. In total, 1400 cumulus–oocyte complexes (COCs) were distributed in four groups and maturated according to supplementation with increasing concentrations of butafosfan (0 mg/ml, 0.05 mg/ml, 0.1 mg/ml and 0.2 mg/ml). Then, 20 oocytes per group were collected to evaluate nuclear maturation and gene expression on cumulus cells and oocytes and the remaining oocytes were inseminated and cultured until day 7, when blastocysts were collected for gene expression analysis. A dose-dependent effect of butafosfan was observed, with decrease of cleavage rate and embryo development with higher doses. No difference between groups was observed in maturation rate and expression of genes related to oocyte quality. Our results suggest that butafosfan is prejudicial for oocytes, compromising cleavage and embryo development.

Keywords

BlastocystCattleGene expressionOocyte competencePhosphorus
Type
Research Article
Information
Zygote , Volume 27 , Issue 5 , October 2019 , pp. 321 - 328
Copyright
© Cambridge University Press 2019 

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