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Differential molecular and hormonal changes in oocytes, granulosa cells and follicular fluid of pregnant and non-pregnant camels

Published online by Cambridge University Press:  07 April 2021

Ahmed M. Fawzy ,
Sally Ibrahim ,
Karima Mahmoud ,
Bassiouni A. Heleil ,
I. El-Kon Ismail ,
Essam A. Almadaly ,
Mohammed A. El-Magd Open the ORCID record for Mohammed A. El-Magd [Opens in a new window]  and
Adel A. Ramoun
Ahmed M. Fawzy
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
Sally Ibrahim
Affiliation:
Department of Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Center, Dokki, Egypt
Karima Mahmoud
Affiliation:
Department of Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Center, Dokki, Egypt
Bassiouni A. Heleil
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
I. El-Kon Ismail
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
Essam A. Almadaly
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
Mohammed A. El-Magd*
Affiliation:
Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
Adel A. Ramoun
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
*
Author for correspondence: Mohammed A. El-Magd. Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt. Email: [email protected]
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Summary

This study aimed to compare the expression of genes regulating follicles development, survival and steroid hormones secretion in oocytes and granulosa cells (GCs) and study the correlation between their expression and follicular fluid (FF) levels of progesterone (P4) in pregnant and non-pregnant camels. In total, 138 ovarian pairs from slaughtered camels were used. Gene expression and hormonal assay were determined using real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The obtained results revealed that the number of follicles (3–8 mm) was significantly (P < 0.05) lower in pregnant, compared with non-pregnant, camels. P4 level in the FF was significantly (P < 0.05) higher in pregnant, compared with non-pregnant, camels. However, no significant (P > 0.05) difference was noticed in the oestradiol (E2) level. STAR, PTEN, IGF1 and BCL2 mRNA levels were significantly higher in GCs and significantly lower in oocytes of pregnant, compared with non-pregnant, camels. However, follicle-stimulating hormone receptor (FSHR) mRNA level was significantly lower in GCs and oocytes, and the BMP15 mRNA level was significantly lower in oocytes of pregnant, compared with non-pregnant, camels. P4 level in FF was positively correlated with STAR, PTEN, IGF1 and BCL2 mRNA levels in GCs and negatively correlated with BMP15 mRNA levels in oocytes and FSHR mRNA levels in GCs and oocytes of pregnant camels. It could be concluded that pregnancy-induced variations in oocytes and GC expression of BMP15, IGF1, FSHR, STAR, BCL2, and PTEN genes might be associated with a decrease in the number of follicles and an increase in the FF level of P4.

Keywords

CamelsGene expressionGranulosa cellsOocytePregnancy
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 427 - 434
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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