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Differential effects of a high-fat diet on serum lipid parameters and ovarian gene expression in young and aged female mice

Published online by Cambridge University Press:  17 February 2016

Driele Neske Garcia ,
Lígia Antunes Prietsch ,
Joao Alveiro Alvarado Rincón ,
Iraê de Lima Moreira ,
Sandra Costa Valle ,
Carlos Castilho Barros ,
Elizabete Helbig ,
Marcio Nunes Corrêa  and
Augusto Schneider
Driele Neske Garcia
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Lígia Antunes Prietsch
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Joao Alveiro Alvarado Rincón
Affiliation:
Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Iraê de Lima Moreira
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Sandra Costa Valle
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Carlos Castilho Barros
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Elizabete Helbig
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Marcio Nunes Corrêa
Affiliation:
Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Augusto Schneider*
Affiliation:
Faculdade de Nutrição, Universidade Federal de Pelotas, Rua Gomes Carneiro, 1 Sala 239 CEP 96010–610 Pelotas–RS, Brazil.
*
All correspondence to: Augusto Schneider. Faculdade de Nutrição, Universidade Federal de Pelotas, Rua Gomes Carneiro, 1 Sala 239 CEP 96010–610 Pelotas–RS, Brazil. Tel: + 55 53 39211270. E-mail: [email protected]
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Summary

The aim of this study was to compare serum lipid profiles and ovarian gene expression between aged and younger female mice fed a control or a high-fat diet for 2 months. For this 16 female mice (C57BL/6) of 4 months (Young, n = 8) or 13 months (Old, n = 8) of age were used. The females were divided into four groups: (i) young females fed a normal diet; (ii) young females fed a high-fat diet; (iii) old females fed a normal diet; and (iv) old females fed a high-fat diet. Food intake was reduced (P < 0.05) in mice fed with a high-fat (2.9 ± 0.1 g) diet in comparison with control mice (3.9 ± 0.1 g). Body weight was higher for old females on the high-fat diet (35.1 ± 0.3 g) than for young females on the same diet (23.3 ± 0.4 g; P < 0.05). PON1 activity was lower in the high-fat than control diet group (114.3 ± 5.8 vs. 78.1 ± 6.0 kU/L, respectively) and was higher in older than younger females (85.9 ± 6.4 vs. 106.5 ± 5.3; P < 0.05, respectively). Females fed a high-fat diet had lower expression of Igf1 mRNA (P = 0.04). There was an interaction between age and diet for the expression of Gdf9 and Survivin, with lower expression in older females in both diets and young females that received the high-fat diet (P < 0.05). Concluding, the high-fat diet reduced the expression of ovarian Igf1 mRNA, and Gdf9 and Survivin mRNA in younger females, which can indicate lower fertility rates. High-density lipoprotein concentration and PON1 activity were higher in aged female mice.

Keywords

AMHFOXO3aIGF-1SIRT1
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 676 - 683
Copyright
Copyright © Cambridge University Press 2016 

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