Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-14T21:27:07.868Z Has data issue: false hasContentIssue false
  • English
  • Français

Different preferences of IVF and SCNT bovine embryos for culture media

Published online by Cambridge University Press:  11 July 2012

Xian-rong Xiong ,
Li-jun Wang ,
Yong-sheng Wang ,
Song Hua ,
Xiang-dong Zi  and
Yong Zhang
Xian-rong Xiong
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
Li-jun Wang
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yong-sheng Wang
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Song Hua
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Xiang-dong Zi
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
Yong Zhang*
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
*
All correspondence to: Yong Zhang. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The preference of fertilized (IVF) and somatic cell nuclear transfer (SCNT) presumptive zygotes for different media when cultured in vitro to the blastocyst stage was evaluated in this study. The experiment comprised two zygote production methods (IVF and SCNT) × two culture media (mSOF and G1.5/G2.5) factorial design in which culture droplets that contained approximate 30 presumptive zygotes formed the experimental plots for the assessment of cleavage and blastocyst development. There were 15 to 20 replicates (culture droplets) per treatment combination. Sub-samples 30 to 41 of the blastocysts produced were assessed for cell number and cell apoptosis. A further 10 blastocysts per treatment combination were used for quantitative real-time polymerase chain reaction (RT-PCR) to evaluate the relative abundance of Hsp70 and Bax mRNA. Presumptive zygotes produced by IVF were developmentally more competent than SCNT zygotes in terms of cleavage rate (66.9 vs. 57.0%; P < 0.05) and blastocyst development rates (blastocysts of presumptive zygotes 29.7 vs. 24.8%; blastocysts of cleaved zygotes 44.4 vs. 36.6%; P < 0.05). Over both zygote production systems, however, the results were similar whether culture was in mSOF or in G1.5/G2.5 media for cleavage rate (63.2 vs. 62.4%; P > 0.05) and blastocyst development rate (blastocysts of presumptive zygotes 26.4 vs. 25.7%; P > 0.05; blastocysts of cleaved zygotes 41.8 vs. 41.2%; P > 0.05). There was, however, a significant interaction between the method of zygote production and culture medium for the apoptotic index of blastocysts. The interaction was such that IVF-produced zygotes cultured in mSOF had a lower apoptotic index compared with those cultured in G1.5/G2.5 (4.7 ± 1.2% vs. 9.8 ± 0.9%; P < 0.05) whereas SCNT zygotes had a higher apoptotic index when cultured in mSOF compared with those cultured in G1.5/G2.5 (11.9 ± 1.5% vs. 4.5 ± 1.2%; P < 0.05). Moreover, RT-PCR analysis showed that embryos from IVF-produced zygotes cultured in mSOF had a lower expression level of stress-related and apoptosis genes (Hsp70 and Bax) than those cells cultured in G1.5/G2.5 medium, while SCNT-derived embryos cultured in mSOF had a higher expression level of these genes than those embryos cultured in G1.5/G2.5 medium. The results of this study show that bovine IVF- and SCNT-produced presumptive zygotes have different nutrient requirements for in vitro culture to the blastocyst stage of development. IVF-derived zygotes have a preference for mSOF as the culture medium whereas the G1.5/G2.5 medium is more suitable for the culture of bovine SCNT-derived zygotes.

Keywords

Bovine embryosG1.5/G2.5In vitro developmentIVFmSOFSCNT
Type
Research Article
Information
Zygote , Volume 22 , Issue 1 , February 2014 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bavister, B. (1995). Culture of preimplantation embryos: facts and artifacts. Hum. Reprod. Update 1, 91–148.CrossRefGoogle Scholar
Bernardini, C., Fantinati, P., Castellani, G., Forni, M., Zannoni, A., Seren, E. & Bacci, M.L. (2003). Alteration of constitutive heat shock protein 70 (HSP70) production by in vitro culture of porcine preimplanted embryos. Vet. Res. Commun. 27, 575–8.CrossRefGoogle ScholarPubMed
Biggers, J.D., McGinnis, L.K. & Lawitts, J.A. (2005). One-step versus two-step culture of mouse preimplantation embryos: is there a difference. Hum. Reprod. 20, 3376–84.Google Scholar
Biggers, J.D., McGinnis, L. & Summers, M.C. (2006). Reply: One-step versus two-step culture of mouse preimplantation embryos. Hum. Reprod. 21, 1936–9.CrossRefGoogle Scholar
Chung, Y.G., Mann, M.R.W., Bartolomei, M.S. & Latham, K.E. (2002). Nuclear–cytoplasmic “tug of war” during cloning: effects of somatic cell nuclei on culture medium preferences of preimplantation cloned mouse embryos. Biol Reprod. 66, 1178–84.CrossRefGoogle ScholarPubMed
Ellington, J.E., Carney, E.W., Farrell, P.B., Simkin, M.E. & Foote, R.H. (1990). Bovine 1–2-cell embryo development using a simple medium in three oviduct epithelial cell coculture systems. Biol. Reprod. 43, 97–104.Google Scholar
Erbach, G.T., Lawitts, J.A., Papaioannou, V.E. & Biggers, J.D. (1994). Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod. 345, 1027–33.CrossRefGoogle Scholar
Farin, P.W., Crosier, A.E. & Farin, C.E. (2001). Influence of systems on embryo survival and fetal development in cattle. Theriogenology 55, 151–70.Google Scholar
Feugang, J.M., Camargo-Rodríguez, O. & Memili, E. (2009). Culture systems for bovine embryos. Livestock Sci. 121, 141–9.CrossRefGoogle Scholar
Fukui, Y., Lee, E.S. & Araki, N. (1996). Effect of medium renewal during culture in two different culture systems on development to blastocysts from in vitro produced early bovine embryos. Anim. Sci. 74, 2752–8.CrossRefGoogle ScholarPubMed
Gandhi, A.P., Lane, M., Gardner, D.K. & Krisher, R.L. (2000). A single medium supports development of bovine embryos throughout maturation, fertilization and culture. Hum. Reprod. 15, 395–401.Google Scholar
Gandhi, A.P., Lane, M., Gardner, D.K. & Krisher, R.L. (2001). Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Mol. Reprod. Dev. 58, 269–75.Google Scholar
Gaomm, S., Chung, Y.G., Williams, J.W., Riley, J., Moley, K. & Latham, K.E. (2003). Somatic cell-like features of cloned mouse embryos prepared with cultured myoblast nuclei. Biol. Reprod. 69, 48–56.Google Scholar
Garcia-Garcia, R.M., Ward, F., Fair, S., O'Meara, C.M., Wade, M., Duffy, P. & Lonergan, P. (2007). Development and quality of sheep embryos cultured in commercial G1.5/G2.5 sequential media. Anim. Reprod. Sci. 98, 233–40.Google Scholar
Gardner, D.K. (1994). Mammalian embryo culture in the absence of serum or somatic cell support. Cell. Biol. Int. 18, 1163–79.CrossRefGoogle ScholarPubMed
Gardner, D.K. & Lane, M. (1997). Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum. Reprod. Update 34, 367–82.Google Scholar
Gjorret, J.O., Avery, B., Larsson, L-I., Schellander, K. & Hyttel, P. (2001). Apoptosis in bovine blastocysts produced in vivo and in vitro. Theriogenology 55, 321.Google Scholar
Gomez, E., Rodrıguez, A., Munoz, M., Caamano, J.N., Hidalgo, C.O., Moran, E., Facal, N. & Diez, C. (2008). Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology 69, 1013–21.CrossRefGoogle ScholarPubMed
Heindryckx, B., Rybouchkin, A., Vander Elst, J. & Dhont, M. (2001). Effect of culture media on in vitro development of cloned mouse embryos. Cloning 3, 41–50.CrossRefGoogle ScholarPubMed
Krisher, R.L. (2004). The effect of oocyte quality on development. Anim. Sci. 82, 14–23.Google Scholar
Lane, M., Gardner, D.K., Hasler, M.J. & Hasler, J.F. (2003). Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-culture. Theriogenology 60, 407–19.CrossRefGoogle ScholarPubMed
Lazzari, G., Wrenzycki, C., Herrmann, D., Duchi, R., Kruip, T., Niemann, H. & Galli, C. (2002). Cellular and molecular deviations in bovine in vitro produced embryos are related to the large offspring syndrome. Biol. Reprod. 67, 767–75.CrossRefGoogle Scholar
Lim, J.M., Reggio, B.C., Godke, R.A. & Hansel, W. (1999). Development of in vitro-derived bovine embryos cultured in 5% CO2 in air or in 5% O2, 5% CO2 and 90% N2. Hum. Reprod. 14, 458–64.Google Scholar
Liu, F.J., Zhang, Y. & Zheng, Y.M. (2007). Optimization of electrofusion protocols for somatic cell nuclear transfer. Small Rum. Res. 73, 246–51.CrossRefGoogle Scholar
Lonergan, P., Rizos, D., Gutierrez-Adan, A., Fair, T. & Boland, M.P. (2003). Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns. Reprod. Domest. Anim. 38, 259–67.Google Scholar
Mastromonaco, G.F., Semple, E., Robert, C., Rho, G.J., Betts, D.H. & King, W.A. (2004). Different culture media requirements of ivf and nuclear transfer bovine embryos. Reprod. Dom. Anim. 39, 462–7.CrossRefGoogle ScholarPubMed
Niemann, H. & Wrenzycki, C. (2000). Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: implications for subsequent development. Theriogenology 53 21–34.CrossRefGoogle ScholarPubMed
Oltval, Z.N., Milliman, C.L. & Korsmeyer, S.J. (1993). Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74, 609–19.CrossRefGoogle Scholar
Pool, T.B. (2004). Development of culture media for human assisted reproductive technology. Fertil. Steril. 81, 287–9.Google Scholar
Rizos, D., Lonergan, P., Boland, M.P., Arroyo-Garcia, R., Pintado, B., Fuente, J. & Gutiérrez-Adán, A. (2002). Analysis of differential messenger RNA expression between bovine blastocysts produced in different culture systems: implications for blastocyst quality. Biol. Reprod. 66, 589–95.Google Scholar
Rizos, D., Clemente, M., Bermejo-Alvarez, P., de La Fuente, J., Lonergan, P. & Gutierrez-Adan, A. (2008). Consequences of in vitro culture conditions on embryo development and quality. Reprod. Dom. Anim. 43, 44–50.CrossRefGoogle ScholarPubMed
Rosenkrans, C.F. & First, N.L. (1991). Culture of bovine zygotes to the blastocyst stage: effects of amino acids and vitamins. Theriogenology 35, 266–7.CrossRefGoogle Scholar
Rutledge, R.G. & Côté, C. (2003). Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res. 31, 16–93.CrossRefGoogle ScholarPubMed
Sagirkaya, H., Misirlioglu, M., Kaya, A., First, N.L., Parrish, J.J. & Memili, E. (2006). Developmental and molecular correlates of bovine preimplantation embryos. Reproduction 131, 895–904.CrossRefGoogle ScholarPubMed
Sepulveda, S., Garcia, J., Arriaga, E., Diaz, J., Noriega-Portella, L. & Noriega-Hoces, L. (2009). In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system. Fertil. Steril. 91, 1765–70.Google Scholar
Steeves, T.E. & Gardner, D.K. (1999). Temporal and differential effects of amino acids on bovine embryo development in culture. Biol. Reprod. 61, 731–40.Google Scholar
Swain, J.E., Bormann, C.L. & Krisher, R.L. (2001). Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1.2/G2.2 sequential medium. Theriogenology 56, 459–69.Google Scholar
Takahashi, Y. & First, N.L. (1992). In vitro development of bovine one cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37, 963–78.CrossRefGoogle ScholarPubMed
Tervit, H.R., Whittingham, D.G. & Rowson, L.E. (1972). Successful culture of in vitro sheep and cattle ova. Reprod. Fertil. 30, 493–7.Google Scholar
Van Langendonckt, A., Demylle, D., Wyns, C., Nisolle, M. & Donnez, J. (2001). Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study. Fertil. Steril. 76, 1023–31.CrossRefGoogle ScholarPubMed
Vireque, A.A., Camargo, L.S.A., Serapiao, R.V., Rosa e Silva, A.A.M., Watanabe, Y.F., Ferreira, E.M., Navarro, P.A.A.S., Martins, W.P. & Ferriani, R.A. (2009). Preimplantation development and expression of Hsp 70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. Theriogenology 71, 620–27.CrossRefGoogle ScholarPubMed
Wrenzycki, C., Herrmann, D., Carnwath, J.W. & Niemann, H. (1999). Alterations in the relative abundance of gene transcripts in preimplantation bovine embryos cultured in medium supplemented with either serum or PVA. Mol. Reprod. Dev. 53, 8–18.3.0.CO;2-K>CrossRefGoogle ScholarPubMed
Wrenzycki, C., Herrmann, D., Keskintepe, L., Martins, A., Sirisathien, S., Brackett, B. & Niemann, H. (2001). Effects of culture system and protein supplementation on mRNA expression in preimplantation bovine embryos. Hum. Reprod. 16, 893–901.CrossRefGoogle Scholar
Wyllie, A.H. (1995). The genetic regulation of apoptosis. Curr. Opin. Genet. Dev. 5, 97–104.Google Scholar
Xu, J.S., Chan, S.T.H., Lee, W.W., Lee, K.F. & Yeung, W.S.B. (2004). Differential growth, cell proliferation, and apoptosis of mouse embryo in various culture media and in coculture. Mol. Reprod. Dev. 68, 72–80.Google Scholar
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31, 3406–15.CrossRefGoogle ScholarPubMed