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Developmental competence of cat (Felis domesticus) oocytes and embryos after parthenogenetic stimulation using different methods

Published online by Cambridge University Press:  22 February 2018

Joanna Kochan ,
Agnieszka Nowak ,
Wojciech Niżański ,
Sylwia Prochowska ,
Anna Migdał ,
Wiesława Młodawska ,
Agnieszka Partyka  and
Maciej Witkowski
Joanna Kochan
Affiliation:
University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, Krakow, Poland.
Agnieszka Nowak*
Affiliation:
University of Agriculture in Krakow, Faculty of Animal Sciences, Institut of Veterinary Sciences, al. Mickiewicza 21, 30–120 Krakow, Poland.
Wojciech Niżański
Affiliation:
Wroclaw University of Environmental and Life Sciences, Department of Reproduction and Clinic of Farm Animals, Wroclaw, Poland.
Sylwia Prochowska
Affiliation:
Wroclaw University of Environmental and Life Sciences, Department of Reproduction and Clinic of Farm Animals, Wroclaw, Poland.
Anna Migdał
Affiliation:
University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, Krakow, Poland.
Wiesława Młodawska
Affiliation:
University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, Krakow, Poland.
Agnieszka Partyka
Affiliation:
Wroclaw University of Environmental and Life Sciences, Department of Reproduction and Clinic of Farm Animals, Wroclaw, Poland.
Maciej Witkowski
Affiliation:
University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, Krakow, Poland.
*
All correspondence to: Agnieszka Nowak. University of Agriculture in Krakow, Faculty of Animal Sciences, Institut of Veterinary Sciences, al. Mickiewicza 21, 30–120 Krakow, Poland. E-mail [email protected]
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Summary

The aim of this study was to compare the effects of various activating factors on feline oocytes. The study included activation within the ovary (natural), activation during in vitro maturation (spontaneous activation), chemical activation (ionomycin + 6-DMAP), activation by spermatozoa and injection (ICSI) and mechanical activation (sham ICSI). According to our results, parthenogenetic embryos could emerge at every step of in vitro embryo production (IVP) procedures. After oocyte collection, 6% of parthenogenetic embryos were observed, mainly at the 2–4-blastomere stages. After 24 h of in vitro maturation, parthenogenetic activation was observed in 7% of oocytes. Using ionomycin and 6-DMAP to artificially activate oocytes, 53% of cleaved embryos were obtained. The results after ICSI (54% cleaved embryos) were not significantly different from the results in Group III using chemical activation (53% cleaved embryos). But only after ICSI were blastocysts obtained (5/73.7%) as a result of in vitro culture. Moreover, embryos after ICSI were of the best morphological quality with minor levels of fragmentation evident in the embryos. After sham mechanical activation, ‘sham ICSI’, 8% of cleaved embryos were noted. Therefore, it is advised to maintain a negative control in parallel with each step of IVP techniques, to avoid misleading results. Chemical methods for artificial activation of feline oocytes are the most promising for application to the cloning and production of parthenogenetic embryos for experimental studies.

Keywords

Feline oocytesICSIOocyte activationParthenogenesis
Type
Research Article
Information
Zygote , Volume 26 , Issue 2 , April 2018 , pp. 119 - 126
Copyright
Copyright © Cambridge University Press 2018 

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