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Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei

Published online by Cambridge University Press:  05 April 2012

Takayuki Yamochi ,
Yuta Kida ,
Noriyoshi Oh ,
Sei Ohta ,
Tomoko Amano ,
Masayuki Anzai ,
Hiromi Kato ,
Satoshi Kishigami ,
Tasuku Mitani  and
Kazuya Matsumoto
...Show all authors
Takayuki Yamochi
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Yuta Kida
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Noriyoshi Oh
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Sei Ohta
Affiliation:
Eve Bioscience Co. Ltd, Wakayama, 648–0003, Japan.
Tomoko Amano
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Masayuki Anzai
Affiliation:
Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
Hiromi Kato
Affiliation:
Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
Satoshi Kishigami
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Tasuku Mitani
Affiliation:
Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
Kazuya Matsumoto
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan. Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
Kazuhiro Saeki
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan. Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
Makoto Takenoshita
Affiliation:
Eve Bioscience Co. Ltd, Wakayama, 648–0003, Japan.
Akira Iritani
Affiliation:
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan.
Yoshihiko Hosoi*
Affiliation:
Department of Genetic Development, Kinki University, Wakayama 649-6493, Japan. Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan. Institute of Advanced Technology, Kinki University, Wakayama, 642-0017, Japan.
*
All correspondence to: Y. Hosoi. Department of Genetic Development, Kinki University, Wakayama 649-6493, Japan. Tel: +81 736 77 3888. Fax: +81 736 77 4754. e-mail: [email protected]
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Summary

Interspecies somatic cell nuclear transfer (ISCNT) has been proposed as a technique to produce cloned offspring of endangered species as well as to investigate nucleus–cytoplasm interactions in mammalian embryo. However, it is still not known which embryo culture medium is optimal for ISCNT embryos for the nuclear donor or the oocyte recipient. We assessed the effects of the culture medium on the developmental competence of the ISCNT embryos by introducing cynomolgus monkey (Macaca fascicularis) fibroblast nuclei into enucleated rabbit (Oryctolagus cuniculus) oocytes (monkey–rabbit embryo). The monkey–rabbit ISCNT embryos that were cultured in mCMRL-1066 developed to the blastocyst stage, although all monkey–rabbit ISCNT embryos cultured in M199 were arrested by the 4-cell stage. When monkey–rabbit ISCNT and rabbit–rabbit somatic cell nuclear transfer (SCNT) embryos were cultured in mCMRL-1066, the blastocyst cell numbers of the monkey–rabbit ISCNT embryos corresponded to the cell numbers of the control rabbit–rabbit SCNT embryos, which were produced from a rabbit fibroblast nucleus and an enucleated rabbit oocyte. In addition, the presence of mitochondria, which were introduced with monkey fibroblasts into rabbit recipient cytoplasm, was confirmed up to the blastocyst stage by polymerase chain reaction (PCR). This study demonstrated that: (1) rabbit oocytes can reprogramme cynomolgus monkey somatic cell nuclei, and support preimplantation development; (2) monkey–rabbit ISCNT embryos developed well in monkey culture medium at early embryonic developmental stages; (3) the cell number of monkey–rabbit ISCNT embryos is similar to that of rabbit–rabbit SCNT embryos; and (4) the mitochondrial fate of monkey–rabbit ISCNT embryos is heteroplasmic from the time just after injection to the blastocyst stage that has roots in both rabbit oocytes and monkey fibroblasts.

Keywords

Culture mediumCynomolgus monkeyEmbryo developmentInterspecies nuclear transferRabbit
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 358 - 366
Copyright
Copyright © Cambridge University Press 2012 

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