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Development and loss of the ability of mouse oolemma to fuse with spermatozoa

Published online by Cambridge University Press:  26 September 2008

Maurizio Zuccotti ,
Anna Piccinelli ,
Nicola Marziliano ,
Silvia Mascheretti  and
Carlo Alberto Redi
Maurizio Zuccotti*
Affiliation:
Dipartimento di Biologia Arimale and centro di studio per I'Istochimic del CNR, University of pavia, Italy
Anna Piccinelli
Affiliation:
Dipartimento di Biologia Arimale and centro di studio per I'Istochimic del CNR, University of pavia, Italy
Nicola Marziliano
Affiliation:
Dipartimento di Biologia Arimale and centro di studio per I'Istochimic del CNR, University of pavia, Italy
Silvia Mascheretti
Affiliation:
Dipartimento di Biologia Arimale and centro di studio per I'Istochimic del CNR, University of pavia, Italy
Carlo Alberto Redi
Affiliation:
Dipartimento di Biologia Arimale and centro di studio per I'Istochimic del CNR, University of pavia, Italy
*
Maurizio Zuccotti, Dipartimento di Biologia Animale, Laboratorio Istologia ed Embriologia, University of Pavia, Piazza Botta 10, I-27100 Pavia, Italy. Telephone: +/382/386323. Fax: +/382/386290. e-Mail: ANTRO at IPVCCN.BITNET.
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Summary

To further our Knowledge on the mechanisms and molecules involved in mouse sperm–oocyte plasma membrane interaciton, exteraction, experiments were carried out to determine the stage during oogenesis at which an oocte acquires the capacity ot fuse with acrosome-reacted sperm. Zona-ferr oocytes 10 μm in diametes do not fuse with sperm. Oolemma fusibility is first acquired when the oocyte reaches about 20μm in diameter. Fusibility is maniatained even after fertilisation has accurred and is lost completely by the 4–cell stage.

Keywords

FertilisationMembrane fusionOolemmapolyspermy block
Type
Article
Information
Zygote , Volume 2 , Issue 4 , November 1994 , pp. 333 - 339
Copyright
Copyright © Cambridge University Press 1994

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References

Blobel, C.P., Wolfsberg, T.G., Turck, C.W., Myles, D.G., Promakoff, P.,& White, J.M., (1992). A potential fusion perptide and an integrin ligand domain in a protein active in sperm–egg fusion. Nature 356, 248–52.CrossRefGoogle Scholar
Boldt, J., Gunter, L.E. & Howe, A.M.. (1989). Characterization of cell surface polypeptides of unfertilized, fertilized, and protease treated zona–free mouse eggs. Gamete Res 24, 471–81.Google Scholar
Fulton, B.P.& Whittingham, D.G.. (1978). Activation of manmalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle Scholar
Hinkley, R., Wright, B.D. &Lynn, J.W.. (1986). Rapid visual detection of sperm–egg fusion using the DNA–specific fluorochrome Hoechst 33342 Dev. Biol 118 148–54.CrossRefGoogle ScholarPubMed
Horvath, P.M., Kellom, T., Caulfield, J., & Boldt, J. (1993). Mechenistic studies of the plasm membrane block to polyspermy in mouse eggs. Mol. Reprod. Dev 34. 65–72.CrossRefGoogle Scholar
Kellom, T.A., Vick, A. &Boldt, J. (1992). Recovery of sperm penetration ablitu in protease treated zona-free mouse eggs occurs coincident with recovery of cell surface 94 kD protein Mol Reprod. Dev 33, 46–52.CrossRefGoogle ScholarPubMed
Monk, M. (1987). Mammalian Development: A practical Approach Oxford, IRL Press.Google Scholar
Myles, D.G., Kimmel, L.H., Blobel, C.P., White, J.M. &primaKoff, P. (1994). Identification of abinding site in the disintegrin domain of fertilin required for sperm-egg fusion proc. Natl. Acad.sci. USA 91 4195–8.CrossRefGoogle Scholar
Stewardsavage, J. & Bavister, B.D.. (1988). A cell surface block to polyspermy occurs in the golden hamster eggs. Dev. Biol 128 150–7.CrossRefGoogle Scholar
Toyoad, y., Yokoyama, M. & Hoshi, T., (1971). studies on the fertilizaion of mouse eggs in II.Effects of in vitro preincubation of spermatozoa on time of sperm penetration of mouse eggs in vitro. Jpm.j. Anim. Reprod 16, 152–7.CrossRefGoogle Scholar
Wassarman, P. (1988). The mammalian ovum In The physiology of Reproduction Knobil, E &Neill, JD. New YorkRaven press. 69–102.Google Scholar
White, J.M. (1990). Viral and cellular membrane fusion proteis Annu. Rev. Physiol 52, 675–97.CrossRefGoogle Scholar
Yanagimachi, R. (1988). Mammalian fertilization In The physiology of Reproduction Knobil, E & Neill, JD 1, 135–85, New YorkReven Press.Google Scholar
Yanagimachi, R.. (1994). Mammalian fertilization. In The physiology of Reproduction, 2nd edn.Knobil, E. & Neill, J.D.. 1 189–317New YorkReven Press.Google Scholar
Zuccotti, M., Yanagimachi, H., & Yanagimachi, R. (1991a). The ablitiy of hamster oolemma to fuse with spermatozoa: its acwusition during oogenesis and loss after fertilization. Development. 112 143–52.CrossRefGoogle Scholar
Zuccotti, M., Urch, U.A. & Yanagimachir, r. (1991b). collagenase as an agent for dissolving the zona pellucida of hamaster and mouse oocytes J. Reprod. Fert 93, 515–20.CrossRefGoogle Scholar