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Determination of the ideal volume of activating solution and the optimal ratio of spermatozoa per oocyte for Prochilodus brevis fertilization

Published online by Cambridge University Press:  18 November 2019

Mayara Setúbal Oliveira-Araújo Open the ORCID record for Mayara Setúbal Oliveira-Araújo [Opens in a new window] ,
Júlia Trugilio Lopes ,
Larissa Teixeira Nunes ,
Priscila Silva de Almeida-Monteiro ,
Renata Vieira do Nascimento ,
Vanessa Alves Pereira ,
Yasmim Maia Ferreira ,
Raphael William Ponte Neres ,
Assis Rubens Montenegro  and
Liliane Veras Leite-Castro
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Mayara Setúbal Oliveira-Araújo*
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Júlia Trugilio Lopes
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Larissa Teixeira Nunes
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Priscila Silva de Almeida-Monteiro
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Renata Vieira do Nascimento
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Vanessa Alves Pereira
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Yasmim Maia Ferreira
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Raphael William Ponte Neres
Affiliation:
Faculty of Veterinary Medicine, State University of Ceará, UECE, Fortaleza, CE, Brazil
Assis Rubens Montenegro
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Liliane Veras Leite-Castro
Affiliation:
Dept of Biology, Federal Institute of Education, Science and Technology of Ceará, IFCE, Acopiara, CE, Brazil
Carminda Sandra Brito Salmito-Vanderley
Affiliation:
Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, Brazil
*
Author for correspondence: Mayara Setúbal Oliveira-Araújo, Faculty of Veterinary Medicine, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil. Tel:/Fax: +85 3101 9851. E-mail: [email protected]
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Summary

Prochilodus brevis is a rheophilic species with a threatened natural population that promotes studies aimed at optimizing reproduction in captivity. The correct quantity of inseminating dose and activating solution volume significantly improves fertilization rates, thereby increasing productivity in captivity. The objective of this study was to determine the proportion of sperm per oocyte and the ideal volume of activating solution to be used in the assisted fertilization of P. brevis. Gametes were collected and fertilization performed in two steps. In step 1, the ideal proportion of spermatozoa was determined based on the fertilization rate:oocyte by testing six doses of semen: D1 = 30 × 103, D2 = 150 × 103, D3 = 300 × 103, D4 = 3 × 106, D5 = 5 × 106, and D6 = 10 × 106. In step 2, the fertilization and hatching rates were evaluated using different volumes of activating solution (V1 – 25 ml, V2 – 50 ml, V3 – 75 ml,V4 – 100 ml, V5 – 125 ml, and V6 – 150 ml). A linear regression equation was estimated from steps 1 and 2. The Student–Newman–Keuls test was used to compare the means. In step 1, the percentage of fertilization increased linearly, reaching a plateau of 51.69%. In step 2, the best fertilization rates were obtained with an estimated ideal volume of 75.64 ml per 2 ml of oocytes. Therefore, the proportion of 928,410.29 sperm:oocyte, associated with the volume of 75.64 ml of water per 2 ml of oocytes, provided the maximum reproductive performance for P. brevis.

Keywords

Assisted reproductionBrazilian bocachicoCharacidaeFreshwater fishInseminating dose
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 103 - 108
Copyright
© Cambridge University Press 2019

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