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Decrease in CD9 content and reorganization of microvilli may contribute to the oolemma block to sperm penetration during fertilization of mouse oocyte

Published online by Cambridge University Press:  26 November 2009

Eliza Żyłkiewicz ,
Julita Nowakowska  and
Marek Maleszewski
Eliza Żyłkiewicz
Affiliation:
Department of Embryology, Institute of Zoology, Faculty of Biology, University of Warsaw, Poland.
Julita Nowakowska
Affiliation:
Laboratory of Electron and Confocal Microscopy, Faculty of Biology, University of Warsaw, Poland.
Marek Maleszewski*
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Miecznikowa 1, 02–096 Warszawa, Poland. Department of Embryology, Institute of Zoology, Faculty of Biology, University of Warsaw, Poland.
*
All correspondence to: Department of Embryology, Institute of Zoology, University of Warsaw, Miecznikowa 1, 02–096 Warszawa, Poland. Fax: +48 22 55 41 210. e-mail: [email protected]
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Summary

Tetraspanin CD9 is the only protein of the oocyte membrane (oolemma) known to be required for the fusion of gametes during fertilization in the mouse. Using electron microscopy and immunostaining we examined the differences in localization of CD9 between ovulated oocytes, zygotes and parthenogenetically activated eggs (parthenogenotes). Changes in ultrastructure of oolemma, which take place in oocytes after fertilization or artificial activation, were also assessed. We demonstrated that after fertilization the level of CD9 present on microvilli of zygote was two times lower than its level on the oolemma of the oocyte. In addition, we showed that the distribution of microvilli is less uniform in the zygotes than in the unfertilized oocytes. We propose that the changes of microvilli distribution and their CD9 content are responsible for the development of the oocyte membrane block to sperm penetration.

Keywords

Block to polyspermyElectron microscopyFertilizationGamete fusion
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 195 - 201
Copyright
Copyright © Cambridge University Press 2009

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