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A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology

Published online by Cambridge University Press:  11 July 2012

Dun-Gao Li ,
Yan Zhu ,
He-Ping Li ,
Xue-Jin Chen  and
Man-Xi Jiang
Dun-Gao Li
Affiliation:
Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China.
Yan Zhu
Affiliation:
Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Committee, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
He-Ping Li
Affiliation:
College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China.
Xue-Jin Chen*
Affiliation:
Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Man-Xi Jiang*
Affiliation:
Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
*
All correspondence to: Man-Xi Jiang or Xue-Jin Chen. Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Tel: +86 21 63846590 ext. 776539. e-mail: [email protected] or [email protected]
All correspondence to: Man-Xi Jiang or Xue-Jin Chen. Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Tel: +86 21 63846590 ext. 776539. e-mail: [email protected] or [email protected]
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Summary

The maintenance and preservation of strains of mice used in biomedical research presents a unique challenge to individual investigators and research institutions. The goal of this study was to assess a comprehensive system for mouse strain conservation through a combination of natural mating, sperm cryopreservation and assisted reproductive technology. Our strategy was based on the collection and cryopreservation of fresh epididymal sperm from male mice by semi-vasectomy; these mice were then naturally mated for breeding purposes. If no satisfactory results were obtained from natural breeding, then the cryopreserved sperm were used for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI); resultant embryos were then transferred into pseudopregnant-recipient female mice. Our results show that some semi-vasectomized mouse strains can be conserved by natural breeding, and that sterile males can be compensated for through the use of IVF and ICSI technology. As such, we believe this system is suitable for the purpose of strain conservation, allowing the continuation of natural breeding with the safeguard of assisted reproduction available.

Keywords

Intracytoplasmic sperm injectionIn vitro fertilizationMouseSemi-vasectomyStrain conservation
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 132 - 137
Copyright
Copyright © Cambridge University Press 2012 

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