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Comparison of method for activating mouse oocytes for spermatid nucleus tranfer

Published online by Cambridge University Press:  26 September 2008

Isoji Sasagawa  and
R. Yanagimachi
Isoji Sasagawa*
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Hawaii, USA.
R. Yanagimachi
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Hawaii, USA.
*
Isoji Sasagawa, Department of Urology, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata-shi, Yamagata 990-23, Japan. Fax: +81-236-285370.
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Summary

In the mouse, mature oocytes injected with prespermatozoal cell nuclei remain unactivated. Additional stimulation is needed to trigger oocyte activation leading to embryo development. We compared various electrical stimulations, treatment with cycloheximide alone or in combination with electrical stimulation, and injection of sperm-borne oocyte-activating factor (oscillogen) in terms of their oocyte activation and embryo development rates. Of all the treatments tested, a single electrical pulse (1.0 kV / cm, 128 μs) was the simplest, yet very effective, in allowing the development of the oocytes injected with spermatid nuclei.

Keywords

CycloheximideElectrostimulationMouseOocyte activationOscillogenSpermatid
Type
Article
Information
Zygote , Volume 4 , Issue 4 , November 1996 , pp. 269 - 274
Copyright
Copyright © Cambridge University Press 1996

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