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Comparative glucose and fructose incorporation and conversion by in vitro Produced bovine embryos

Published online by Cambridge University Press:  26 September 2008

Catherine Guyader-Joly ,
Chaqué Khatchadourian  and
Yves Ménézo
Catherine Guyader-Joly*
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France.
Chaqué Khatchadourian
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France.
Yves Ménézo
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France.
*
Y. Ménézo, INSA, Unité de Biologie du développement préimplantaioire, Laboratoire de Biologie Appliquée, Bâtiment 406, 20, avenue Albert Einstein, 69621 Villeurbanne Cedex, France. Telephone: +33/72 43 83 39. Fax: +33/72 43 85 11.
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Summary

We have investigated the quality of bovine IVM/IVF embryos co-cultured on Vero cells. Blastocyst cell numbers are very similar to those obtained in vivo, and higher than those obtained by co-culture with oviduct cells. The metabolism and conversion of fructose and glucose are not equivalent even though carbon dioxide production is similar and increasing from morula to blastocyst. Formation of free amino acids and incorporation into proteins are higher and faster for glucose than for fructose, but this conversion is rather stable with embryonic growth. Moreover, the by-products formed are not the same. Glucose at physiological concentrations (i.e.2 mM)seems to be a more appropriate fuel for the burst of embryonic development at the blastocyst stage in preparation for hatching.

Keywords

GlucoseFructoseConversionMetabolismIn vitro bovine embryos
Type
Article
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 85 - 91
Copyright
Copyright © Cambridge University Press 1996

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