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Characterisation and role of integrins during gametic interaction and egg activation

Published online by Cambridge University Press:  26 September 2008

Céline de Nadai ,
Patrick Fenichel ,
Michèle Donzeau ,
David Epel  and
Brigitte Ciapa
Céline de Nadai
Affiliation:
Faculté de Médecine, Nice, France, and Hopkins Marine Station, Stanford, California, USA.
Patrick Fenichel
Affiliation:
Faculté de Médecine, Nice, France, and Hopkins Marine Station, Stanford, California, USA.
Michèle Donzeau
Affiliation:
Faculté de Médecine, Nice, France, and Hopkins Marine Station, Stanford, California, USA.
David Epel
Affiliation:
Faculté de Médecine, Nice, France, and Hopkins Marine Station, Stanford, California, USA.
Brigitte Ciapa*
Affiliation:
Faculté de Médecine, Nice, France, and Hopkins Marine Station, Stanford, California, USA.
*
B. Ciapa, Groupe de Recherche Sur l'Interaction Gamétique, Faculté de Médecine, Avenue de Valombrose, F-06107 Nice Cedex 02, France.
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Summary

It has recently been proposed that some of the processes induced by fertilisation in mammals may be mediated by integrins. By peforming immunofluorescence labelling and Western blots with antibodies directed against some of the α and β subunits of integrins, we show here the presence of some of these proteins in human and hamster oocytes. Among them, α2 and α5 were also present on in vitro preparations of sea urchin egg cortices. In addition, antibodies raised against these two proteins were the most effective at inhibiting attachment and fusion of human spermatozoa with hamster oocytes. We suggest that α2 and α5 integrin chains may be common mediators in adhesion-fusion mechanisms triggered by fertilisation. Using similar techniques, we show that eggs are rich three cytoskeletal proteins known to be linked to the β chain of integrins: talin, vinculin and α-actinin. Moreover, we found that talin and α-actinin were associated with proteins phosphorylated on tyrosine after fertilisation in sea urchin eggs. We suggest that integrins might be involved during fertilisation and trigger egg activation through cytoskeletal structures.

Keywords

CytoskeletonEggFertilisationIntegrinTalinVinculin
Type
Article
Information
Zygote , Volume 4 , Issue 1 , February 1996 , pp. 31 - 40
Copyright
Copyright © Cambridge University Press 1996

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