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Cat fertilization by mouse sperm injection

Published online by Cambridge University Press:  27 July 2011

Yong-Xun Jin ,
Xiang-Shun Cui ,
Xian-Feng Yu ,
Sung-Hyun Lee ,
Qing-Ling Wang ,
Wei-Wei Gao ,
Yong-Nan Xu ,
Shao-Chen Sun ,
IL-Keun Kong  and
Nam-Hyung Kim
Yong-Xun Jin
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Xiang-Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Xian-Feng Yu
Affiliation:
Laboratory Animal Center, Jilin University, Changchun, Jilin Province, 130062, China.
Sung-Hyun Lee
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Qing-Ling Wang
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Wei-Wei Gao
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Yong-Nan Xu
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Shao-Chen Sun
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
IL-Keun Kong
Affiliation:
Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, GyeongNam, South Korea.
Nam-Hyung Kim*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
*
All correspondence to: Nam-Hyung Kim. Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea. Tel: +82 43 261 2546. Fax: +82 43 272 8853. E-mail: [email protected]
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Summary

Interspecies intracytoplasmic sperm injection has been carried out to understand species-specific differences in oocyte environments and sperm components during fertilization. While sperm aster organization during cat fertilization requires a paternally derived centriole, mouse and hamster fertilization occur within the maternal centrosomal components. To address the questions of where sperm aster assembly occurs and whether complete fertilization is achieved in cat oocytes by interspecies sperm, we studied the fertilization processes of cat oocytes following the injection of cat, mouse, or hamster sperm. Male and female pronuclear formations were not different in the cat oocytes at 6 h following cat, mouse or hamster sperm injection. Microtubule asters were seen in all oocytes following intracytoplasmic injection of cat, mouse or hamster sperm. Immunocytochemical staining with a histone H3-m2K9 antibody revealed that mouse sperm chromatin is incorporated normally with cat egg chromatin, and that the cat eggs fertilized with mouse sperm enter metaphase and become normal 2-cell stage embryos. These results suggest that sperm aster formation is maternally dependent, and that fertilization processes and cleavage occur in a non-species specific manner in cat oocytes.

Keywords

CentrosomeICSIInterspeciesMouse embryoSperm injection
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 371 - 378
Copyright
Copyright © Cambridge University Press 2011

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