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Births of calves derived from embryos produced by intracytoplasmic sperm injection without exogenous oocyte activation

Published online by Cambridge University Press:  19 June 2002

Hong Wei  and
Yukata Fukui
Hong Wei
Affiliation:
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
Yukata Fukui
Affiliation:
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
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Abstract

Tail-cut bovine spermatozoa were microinjected into ooplasmic lipid polarised, in vitro matured bovine oocytes using a piezomicropipette-driving system. No exogenous oocyte activation treatment was used. Of the sperm-injected oocytes, 86.3% were activated, 71.8% cleaved and 22.7% developed to the blastocyst stage. The average cell count of the blastocysts was 122.5 ± 15 and a majority (81.8%) of the blastocysts were cytologically normal (diploid). When transferred to recipient cows, 5 of 8 blastocysts developed to fetuses and 4 of 7 recipients became pregnant. Normal offspring were born.

Keywords

Bovine oocytesICSIPiezoPVPSpermatozoonSperm tail-cutting
Type
Research Article
Information
Zygote , Volume 10 , Issue 2 , May 2002 , pp. 149 - 153
Copyright
2002 Cambridge University Press

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