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Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

Published online by Cambridge University Press:  06 May 2010

Yoshikazu Nagao ,
Yumiko Harada ,
Mari Yamaguchi ,
Akane Igarashi ,
Yuki Ooshima  and
Yoku Kato
Yoshikazu Nagao*
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan. Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan.
Yumiko Harada
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Mari Yamaguchi
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Akane Igarashi
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Yuki Ooshima
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Yoku Kato
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan.
*
All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. Tel/Fax: +81 285 84 1321. e-mail: [email protected]
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Summary

The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ovaries by examining the effects of temperature, solution and supplementation. In Experiment 1, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved at 15°C was higher than that at either 5 or 25°C (p < 0.05). In Experiment 2, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved in University of Wisconsin solution was higher than when PBS or saline was used (p < 0.05). In Experiment 3, oocytes preserved in saline supplemented with 0.3 mM glutathione (GSH) exhibited an increase in the rate of blastocyst formation compared with oocytes supplemented with 0 or 3 mM GSH (p < 0.05). In Experiment 4, supplementation with 10 μM epigallocatechin gallate during ovary preservation increased the rate of blastocyst formation (p < 0.05). The blastocysts derived from ovaries stored in saline supplemented with GSH at 15°C for 24 h were shown to develop into normal offsprings following transfer to recipient heifers. Our studies indicate that bovine IVM/IVF embryos derived from ovaries preserved in saline supplemented with an antioxidant at 15°C for 24 h can successfully develop to the blastocyst stage and result in offspring.

Keywords

AntioxidantBovineOocyteOvaryPreservation
Type
Research Article
Information
Zygote , Volume 18 , Issue 4 , November 2010 , pp. 315 - 321
Copyright
Copyright © Cambridge University Press 2010

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