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Amburana cearensis leaf extract maintains survival and promotes in vitro development of ovine secondary follicles

Published online by Cambridge University Press:  17 June 2015

R.S. Barberino ,
V.R.P. Barros ,
V.G. Menezes ,
L.P. Santos ,
V.R. Araújo ,
M.A.A. Queiroz ,
J.R.G.S. Almeida ,
R.C. Palheta Jr  and
M.H.T. Matos
R.S. Barberino
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
V.R.P. Barros
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
V.G. Menezes
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
L.P. Santos
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
V.R. Araújo
Affiliation:
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, CE, Brazil.
M.A.A. Queiroz
Affiliation:
Laboratory of Bromatology and Animal Nutrition, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
J.R.G.S. Almeida
Affiliation:
Nucleus for Studies and Research on Medicinal Plants, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
R.C. Palheta Jr
Affiliation:
Laboratory of Pharmacology, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
M.H.T. Matos*
Affiliation:
Universidade Federal do Vale do São Francisco (UNIVASF). Campus de Ciências Agrárias. Colegiado de Medicina Veterinária. Laboratório de Biologia Celular, Citologia e Histologia. Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1. CEP: 56300–990, Petrolina, PE, Brasil. Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
*
All correspondence to: M.H.T. Matos. Universidade Federal do Vale do São Francisco (UNIVASF). Campus de Ciências Agrárias. Colegiado de Medicina Veterinária. Laboratório de Biologia Celular, Citologia e Histologia. Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1. CEP: 56300–990, Petrolina, PE, Brasil. Tel: +55 87 2101 4839. E-mail: [email protected]
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Summary

The antioxidant properties of Amburana cearensis extract may be a useful substitute for standard cell culture medium. Thus, the aim of this study was to evaluate the effect of this extract, with or without supplementation, on in vitro survival and development of sheep isolated secondary follicles. After collection of the ovaries, secondary follicles were isolated and cultured for 18 days in α-MEM+ supplemented with bovine serum albumin, insulin, transferrin, selenium, glutamine, hypoxanthine and ascorbic acid (control medium) or into medium composed of different concentrations of A. cearensis extract without supplements (Amb 0.1; 0.2 or 0.4 mg/ml) or A. cearensis extract supplemented with the same substances described above for α-MEM+ supplementation. The A. cearensis supplemented medium was named Amb 0.1+; 0.2+ or 0.4+ mg/ml. There were more morphologically normal follicles in Amb 0.1 or Amb 0.4 mg/ml than in the control medium (α-MEM+) after 18 days of culture. Moreover, the percentage of antrum formation was significantly higher in Amb 0.1 or Amb 0.2 mg/ml than in α-MEM+ and Amb 0.1+ mg/ml, and similar to the other treatments. All A. cearensis extract media induced a progressive and significant increase in follicular diameter throughout the culture period. In conclusion, this study showed that 0.1 mg/ml of this extract, without supplementation, maintains follicular survival and promotes the development of ovine isolated secondary follicles in vitro. This extract can be an alternative culture medium for preantral follicle development.

Keywords

CultureMedicinal plantOocyteSheepUmburana
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 277 - 285
Copyright
Copyright © Cambridge University Press 2015 

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